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Apolipoprotein E (APOE) (NM_000041) Human Tagged ORF Clone
CAT＃: RC200395
APOE (Myc-DDK-tagged)-Human apolipoprotein E (APOE)
ORF Plasmid: tGFP
"NM_000041" in other vectors (6)
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CNY 2,400.00
CNY 4,465.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AD2; APO-E; ApoE4; LDLCQ5; LPG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200395 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTTCTGTGGGCTGCGTTGCTGGTCACATTCCTGGCAGGATGCCAGGCCAAGGTGGAGCAAGCGG TGGAGACAGAGCCGGAGCCCGAGCTGCGCCAGCAGACCGAGTGGCAGAGCGGCCAGCGCTGGGAACTGGC ACTGGGTCGCTTTTGGGATTACCTGCGCTGGGTGCAGACACTGTCTGAGCAGGTGCAGGAGGAGCTGCTC AGCTCCCAGGTCACCCAGGAACTGAGGGCGCTGATGGACGAGACCATGAAGGAGTTGAAGGCCTACAAAT CGGAACTGGAGGAACAACTGACCCCGGTGGCGGAGGAGACGCGGGCACGGCTGTCCAAGGAGCTGCAGGC GGCGCAGGCCCGGCTGGGCGCGGACATGGAGGACGTGTGCGGCCGCCTGGTGCAGTACCGCGGCGAGGTG CAGGCCATGCTCGGCCAGAGCACCGAGGAGCTGCGGGTGCGCCTCGCCTCCCACCTGCGCAAGCTGCGTA AGCGGCTCCTCCGCGATGCCGATGACCTGCAGAAGCGCCTGGCAGTGTACCAGGCCGGGGCCCGCGAGGG CGCCGAGCGCGGCCTCAGCGCCATCCGCGAGCGCCTGGGGCCCCTGGTGGAACAGGGCCGCGTGCGGGCC GCCACTGTGGGCTCCCTGGCCGGCCAGCCGCTACAGGAGCGGGCCCAGGCCTGGGGCGAGCGGCTGCGCG CGCGGATGGAGGAGATGGGCAGCCGGACCCGCGACCGCCTGGACGAGGTGAAGGAGCAGGTGGCGGAGGT GCGCGCCAAGCTGGAGGAGCAGGCCCAGCAGATACGCCTGCAGGCCGAGGCCTTCCAGGCCCGCCTCAAG AGCTGGTTCGAGCCCCTGGTGGAAGACATGCAGCGCCAGTGGGCCGGGCTGGTGGAGAAGGTGCAGGCTG CCGTGGGCACCAGCGCCGCCCCTGTGCCCAGCGACAATCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200395 protein sequence
Red=Cloning site Green=Tags(s) MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELL SSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEV QAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRA ATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLK SWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000041 |
| ORF Size | 951 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000041.4 |
| RefSeq Size | 1223 bp |
| RefSeq ORF | 954 bp |
| Locus ID | 348 |
| UniProt ID | P02649 |
| Domains | Apolipoprotein |
| Protein Families | Adult stem cells, Druggable Genome, Secreted Protein, Stem cell - Pluripotency |
| Protein Pathways | Alzheimer's disease |
| MW | 36.2 kDa |
| Gene Summary | The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Isoform-dependent lysosomal degradation and internalization of apolipoprotein E requires autophagy proteins
,null,
Journal of Cell Science
,PubMed ID 34982109
[Apolipoprotein E]
|
|
SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE
,Raihan, O;Brishti, A;Li, Q;Zhang, Q;Li, D;Li, X;Zhang, Q;Xie, Z;Li, J;Zhang, J;Liu, Q;,
Cell Rep
,PubMed ID 31269452
[Apolipoprotein E]
|
|
Human ApoE Isoforms Differentially Modulate Brain Glucose and Ketone Body Metabolism: Implications for Alzheimer's Disease Risk Reduction and Early Intervention
,Wu, L;Zhang, X;Zhao, L;,
J. Neurosci.
,PubMed ID 29967007
[Apolipoprotein E]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200395L1 | Lenti ORF clone of Human apolipoprotein E (APOE), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200395L2 | Lenti ORF clone of Human apolipoprotein E (APOE), mGFP tagged |
CNY 5,890.00 |
|
| RC200395L3 | Lenti ORF clone of Human apolipoprotein E (APOE), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200395L4 | Lenti ORF clone of Human apolipoprotein E (APOE), mGFP tagged |
CNY 4,800.00 |
|
| RG200395 | APOE (tGFP-tagged) - Human apolipoprotein E (APOE) |
CNY 4,000.00 |
|
| SC319433 | APOE (untagged)-Human apolipoprotein E (APOE) |
CNY 2,400.00 |
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