MCL1 (NM_021960) Human Tagged ORF Clone

Name: MCL1 (NM_021960) Human Tagged ORF Clone
SKU: RC200521
Price: 5488.00

CAT＃: RC200521

MCL1 (Myc-DDK-tagged)-Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_021960" in other vectors (6)

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CNY 5,488.00

货期*

现货

规格

Cited in 16 publications.

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

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PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

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TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

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TA501036

Anti-MCL1 mouse monoclonal antibody, clone OTI10F6 (formerly 10F6)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC200521 representing NM_021960 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGGCCTCAAAAGAAACGCGGTAATCGGACTCAACCTCTACTGTGGGGGGGCCGGCTTGGGGGCCG GCAGCGGCGGCGCCACCCGCCCGGGAGGGCGACTTTTGGCTACGGAGAAGGAGGCCTCGGCCCGGCGAGA GATAGGGGGAGGGGAGGCCGGCGCGGTGATTGGCGGAAGCGCCGGCGCAAGCCCCCCGTCCACCCTCACG CCAGACTCCCGGAGGGTCGCGCGGCCGCCGCCCATTGGCGCCGAGGTCCCCGACGTCACCGCGACCCCCG CGAGGCTGCTTTTCTTCGCGCCCACCCGCCGCGCGGCGCCGCTTGAGGAGATGGAAGCCCCGGCCGCTGA CGCCATCATGTCGCCCGAAGAGGAGCTGGACGGGTACGAGCCGGAGCCTCTCGGGAAGCGGCCGGCTGTC CTGCCGCTGCTGGAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGACGGGTCACTACCCTCGACGC CGCCGCCAGCAGAGGAGGAGGAGGACGAGTTGTACCGGCAGTCGCTGGAGATTATCTCTCGGTACCTTCG GGAGCAGGCCACCGGCGCCAAGGACACAAAGCCAATGGGCAGGTCTGGGGCCACCAGCAGGAAGGCGCTG GAGACCTTACGACGGGTTGGGGATGGCGTGCAGCGCAACCACGAGACGGCCTTCCAAGGCATGCTTCGGA AACTGGACATCAAAAACGAAGACGATGTGAAATCGTTGTCTCGAGTGATGATCCATGTTTTCAGCGACGG CGTAACAAACTGGGGCAGGATTGTGACTCTCATTTCTTTTGGTGCCTTTGTGGCTAAACACTTGAAGACC ATAAACCAAGAAAGCTGCATCGAACCATTAGCAGAAAGTATCACAGACGTTCTCGTAAGGACAAAACGGG ACTGGCTAGTTAAACAAAGAGGCTGGGATGGGTTTGTGGAGTTCTTCCATGTAGAGGACCTAGAAGGTGG CATCAGGAATGTGCTGCTGGCTTTTGCAGGTGTTGCTGGAGTAGGAGCTGGTTTGGCATATCTAATAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC200521 representing NM_021960 Red=Cloning site Green=Tags(s) MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLT PDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAV LPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKAL ETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKT INQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAGLAYLIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_021960
ORF Size	1050 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021960.5
RefSeq Size	4020 bp
RefSeq ORF	1053 bp
Locus ID	4170
UniProt ID	Q07820
Domains	Bcl-2
Protein Families	Druggable Genome, Transmembrane
MW	37.2 kDa
Gene Summary	This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (16)

The use of this cDNA Clones has been cited in the following citations:
Stabilization of MCL-1 by E3 ligase TRAF4 confers radioresistance ,null, Cell Death & Disease ,PubMed ID 36535926 [MCL1]
SHP-1/STAT3-Signaling-Axis-Regulated Coupling between BECN1 and SLC7A11 Contributes to Sorafenib-Induced Ferroptosis in Hepatocellular Carcinoma ,null, International Journal of Molecular Sciences ,PubMed ID 36232407 [MCL1]
A macrolide from Streptomyces sp. modulates apoptosis and autophagy through Mcl-1 downregulation in human breast cancer cells ,Chiu, CF;Chiu, SJ;Bai, LY;Feng, CH;Hu, JL;Lin, WY;Huang, HY;Weng, JR;, Environmental toxicology ,PubMed ID 33713530 [MCL1]
PD-L1 Is a Tumor Suppressor in Aggressive Endometrial Cancer Cells and Its Expression Is Regulated by miR-216a and lncRNA MEG3 ,null, Frontiers in Cell and Developmental Biology ,PubMed ID 33363153 [MCL1]
Mutant ACTB mRNA 3\'-UTR promotes hepatocellular carcinoma development by regulating miR-1 and miR-29a ,Li, Y;Ma, H;Shi, C;Feng, F;Yang, L;, Cell. Signal. ,PubMed ID 31846694 [MCL1]
miR-153-3p regulates progression of ovarian carcinoma in vitro and in vivo by targeting MCL1 gene ,Li, C;Zhang, Y;Zhao, W;Cui, S;Song, Y;, J. Cell. Biochem. ,PubMed ID 31297886 [MCL1]
Divaricoside Exerts Antitumor Effects, in Part, by Modulating Mcl-1 in Human Oral Squamous Cell Carcinoma Cells ,Weng, J;Bai, L;Chiu, S;Chiu, C;Lin, W;Hu, J;Shieh, T;, Computational and Structural Biotechnology Journal ,PubMed ID 30788081 [MCL1]
Targeting CDK9 and MCL-1 by a new CDK9/p-TEFb inhibitor with and without 5-fluorouracil in esophageal adenocarcinoma ,Tong, Z;Mejia, A;Veeranki, O;Verma, A;Correa, A;Dokey, R;Patel, V;Solis, L;Mino, B;Kathkuda, R;Rodriguez-Canales, J;Lin, S;Krishnan, S;Kopetz, S;Blum, M;Ajani, J;Hofstetter, W;Maru, D;, Ther Adv Med Oncol ,PubMed ID 31384313 [MCL1]
Cyclocommunol induces apoptosis in human oral squamous cell carcinoma partially through a Mcl-1-dependent mechanism ,Weng, JR;Bai, LY;Ko, HH;Tsai, YT;, Phytomedicine [MCL1]
FTY720 Induces Autophagy-Associated Apoptosis in Human Oral Squamous Carcinoma Cells, in Part, through a Reactive Oxygen Species/Mcl-1-Dependent Mechanism ,Bai, LY;Chiu, CF;Chiu, SJ;Chu, PC;Weng, JR;, Sci Rep ,PubMed ID 28717222 [MCL1]
Antitumor effects of cyclin dependent kinase 9 inhibition in esophageal adenocarcinoma ,Tong, Z;Chatterjee, D;Deng, D;Veeranki, O;Mejia, A;Ajani, JA;Hofstetter, W;Lin, S;Guha, S;Kopetz, S;Krishnan, S;Maru, D;, Oncotarget ,PubMed ID 28404924 [MCL1]
USP9X regulates centrosome duplication and promotes breast carcinogenesis ,Li, X;Song, N;Liu, L;Liu, X;Ding, X;Song, X;Yang, S;Shan, L;Zhou, X;Su, D;Wang, Y;Zhang, Q;Cao, C;Ma, S;Yu, N;Yang, F;Wang, Y;Yao, Z;Shang, Y;Shi, L;, Nat Commun ,PubMed ID 28361952 [MCL1]
Mutant BRAF upregulates MCL-1 to confer apoptosis resistance that is reversed by MCL-1 antagonism and cobimetinib in colorectal cancer ,Kawakami, H;Huang, S;Pal, K;Dutta, SK;Mukhopadhyay, D;Sinicrope, FA;, Mol. Cancer Ther. ,PubMed ID 27765849 [MCL1]
ER Stress-Mediated Upregulation of miR-29a Enhances Sensitivity to Neuronal Apoptosis ,Nolan, K;Walter, F;Tuffy, LP;Poeschel, S;Gallagher, R;Haunsberger, S;Bray, I;Stallings, RL;Concannon, CG;Prehn, JH;, Eur. J. Neurosci. ,PubMed ID 26750440 [MCL1]
Dinaciclib, a CDK inhibitor promotes proteasomal degradation of Mcl-1 and enhances ABT-737 mediated cell death in malignant human glioma cell lines ,Jane, EP;Premkumar, DR;Cavaleri, JM;Sutera, PA;Rajasekar, T;Pollack, I;, J. Pharmacol. Exp. Ther. ,PubMed ID 26585571 [MCL1]
A kinase inhibitor screen identifies Mcl-1 and Aurora kinase A as novel treatment targets in antiestrogen-resistant breast cancer cells ,Thrane, S;Pedersen, AM;Thomsen, MB;Kirkegaard, T;Rasmussen, BB;Duun-Henriksen, AK;Lænkholm, AV;Bak, M;Lykkesfeldt, AE;Yde, CW;, Oncogene ,PubMed ID 25362855 [MCL1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC200521L1	Lenti ORF clone of Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,888.00
RC200521L2	Lenti ORF clone of Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,888.00
RC200521L3	Lenti ORF clone of Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,888.00
RC200521L4	Lenti ORF clone of Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,888.00
RG200521	MCL1 (tGFP-tagged) - Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,088.00
SC315538	MCL1 (untagged)-Human myeloid cell leukemia sequence 1 (BCL2-related) (MCL1), nuclear gene encoding mitochondrial protein, transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,488.00