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FH (NM_000143) Human Tagged ORF Clone
CAT＃: RC200614
FH (Myc-DDK-tagged)-Human fumarate hydratase (FH), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_000143" in other vectors (6)
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CNY 5,696.00
| Cited in 6 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FMRD; HLRCC; HsFH; LRCC; MCL; MCUL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200614 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCGAGCACTTCGGCTCCTCGCGCGCTCGCGTCCCCTCGTGCGGGCTCCAGCCGCAGCCTTAGCTT CGGCTCCCGGCTTGGGTGGCGCGGCCGTGCCCTCGTTTTGGCCTCCGAACGCGGCTCGAATGGCAAGCCA AAATTCCTTCCGGATAGAATATGATACCTTTGGTGAACTAAAGGTGCCAAATGATAAGTATTATGGCGCC CAGACCGTGAGATCTACGATGAACTTTAAGATTGGAGGTGTGACAGAACGCATGCCAACCCCAGTTATTA AAGCTTTTGGCATCTTGAAGCGAGCGGCCGCTGAAGTAAACCAGGATTATGGTCTTGATCCAAAGATTGC TAATGCAATAATGAAGGCAGCAGATGAGGTAGCTGAAGGTAAATTAAATGATCATTTTCCTCTCGTGGTA TGGCAGACTGGATCAGGAACTCAGACAAATATGAATGTAAATGAAGTCATTAGCAATAGAGCAATTGAAA TGTTAGGAGGTGAACTTGGCAGCAAGATACCTGTGCATCCCAACGATCATGTTAATAAAAGCCAGAGCTC AAATGATACTTTTCCCACAGCAATGCACATTGCTGCTGCAATAGAAGTTCATGAAGTACTGTTACCAGGA CTACAGAAGTTACATGATGCTCTTGATGCAAAATCCAAAGAGTTTGCACAGATCATCAAGATTGGACGTA CTCATACTCAGGATGCTGTTCCACTTACTCTTGGGCAGGAATTTAGTGGTTATGTTCAACAAGTAAAATA TGCAATGACAAGAATAAAAGCTGCCATGCCAAGAATCTATGAGCTCGCAGCTGGAGGCACTGCTGTTGGT ACAGGTTTAAATACTAGAATTGGCTTTGCAGAAAAGGTTGCTGCAAAAGTGGCTGCACTTACAGGCTTGC CTTTTGTCACTGCTCCGAATAAATTTGAAGCTCTGGCTGCTCATGACGCTCTGGTTGAGCTCAGTGGAGC CATGAACACTACTGCCTGCAGTCTGATGAAGATAGCAAATGATATTCGATTTTTGGGTTCTGGTCCTCGG TCAGGTCTGGGAGAATTGATCTTGCCTGAAAATGAACCAGGAAGCAGTATCATGCCAGGCAAGGTGAACC CTACTCAGTGTGAAGCAATGACCATGGTTGCAGCCCAAGTCATGGGGAACCATGTTGCTGTCACTGTCGG AGGCAGCAATGGACATTTTGAGTTGAATGTTTTCAAGCCAATGATGATTAAAAATGTGTTACACTCAGCC AGGCTGCTGGGGGATGCTTCAGTTTCCTTTACAGAAAACTGCGTGGTGGGAATCCAGGCCAATACAGAAA GGATCAACAAGCTGATGAATGAGTCTCTAATGTTGGTGACAGCTCTCAATCCTCATATAGGGTATGACAA GGCAGCAAAGATTGCTAAGACAGCACACAAAAATGGATCAACCTTAAAGGAAACTGCTATCGAACTTGGC TATCTCACAGCAGAGCAGTTTGACGAATGGGTAAAACCTAAGGACATGCTGGGTCCAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200614 protein sequence
Red=Cloning site Green=Tags(s) MYRALRLLARSRPLVRAPAAALASAPGLGGAAVPSFWPPNAARMASQNSFRIEYDTFGELKVPNDKYYGA QTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVV WQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPG LQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVG TGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPR SGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSA RLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELG YLTAEQFDEWVKPKDMLGPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000143 |
| ORF Size | 1530 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000143.4 |
| RefSeq Size | 1877 bp |
| RefSeq ORF | 1533 bp |
| Locus ID | 2271 |
| UniProt ID | P07954 |
| Domains | lyase_1 |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma |
| MW | 54.6 kDa |
| Gene Summary | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008] |
Citations (6)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Clinical and Immunological Profile of Anti-factor H Antibody Associated Atypical Hemolytic Uremic Syndrome: A Nationwide Database
,Puraswani, M;Khandelwal, P;Saini, H;Saini, S;Gurjar, BS;Sinha, A;Shende, RP;Maiti, TK;Singh, AK;Kanga, U;Ali, U;Agarwal, I;Anand, K;Prasad, N;Rajendran, P;Sinha, R;Vasudevan, A;Saxena, A;Agarwal, S;Hari, P;Sahu, A;Rath, S;Bagga, A;,
Front Immunol
,PubMed ID 31231391
[FH]
|
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Correlating Conformational Dynamics with the Von Willebrand Factor Reductase Activity of Factor H Using Single Molecule Force Measurements
,Wijeratne, SS;Nolasco, L;Li, J;Jiang, K;Moake, JL;Kiang, CH;,
J Phys Chem B
,PubMed ID 30351116
[FH]
|
|
Krebs-cycle-deficient hereditary cancer syndromes are defined by defects in homologous-recombination DNA repair
,Sulkowski, PL;Sundaram, RK;Oeck, S;Corso, CD;Liu, Y;Noorbakhsh, S;Niger, M;Boeke, M;Ueno, D;Kalathil, AN;Bao, X;Li, J;Shuch, B;Bindra, RS;Glazer, PM;,
Nat. Genet.
,PubMed ID 30013182
[FH]
|
|
Combined effects of FH (E404D) and ACOX2 (R409H) cause metabolic defects in primary cardiac malignant tumor
,Zhou, X;Xu, M;Zeng, W;Chen, Z;Lu, G;Gong, Y;Finnell, RH;Xiao, H;Qiao, B;Wang, H;,
Cell Death Discov
,PubMed ID 30062063
[FH]
|
|
Characterization of genetic predisposition and autoantibody profile in atypical haemolytic-uraemic syndrome
,Gurjar, BS;Manikanta Sriharsha, T;Bhasym, A;Prabhu, S;Puraswani, M;Khandelwal, P;Saini, H;Saini, S;Verma, AK;Chatterjee, P;Guchhait, P;Bal, V;George, A;Rath, S;Sahu, A;Sharma, A;Hari, P;Sinha, A;Bagga, A;,
Immunology
,PubMed ID 29485195
[FH]
|
|
Nuclear ARRB1 induces pseudohypoxia and cellular metabolism reprogramming in prostate cancer
,Zecchini, V;Madhu, B;Russell, R;Pértega-Gomes, N;Warren, A;Gaude, E;Borlido, J;Stark, R;Ireland-Zecchini, H;Rao, R;Scott, H;Boren, J;Massie, C;Asim, M;Brindle, K;Griffiths, J;Frezza, C;Neal, DE;Mills, IG;,
EMBO J.
,PubMed ID 24837709
[FH]
|
Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200614L1 | Lenti ORF clone of Human fumarate hydratase (FH), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 8,096.00 |
|
| RC200614L2 | Lenti ORF clone of Human fumarate hydratase (FH), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 8,096.00 |
|
| RC200614L3 | Lenti ORF clone of Human fumarate hydratase (FH), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 8,096.00 |
|
| RC200614L4 | Lenti ORF clone of Human fumarate hydratase (FH), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 8,096.00 |
|
| RG200614 | FH (tGFP-tagged) - Human fumarate hydratase (FH), nuclear gene encoding mitochondrial protein |
CNY 7,296.00 |
|
| SC319415 | FH (untagged)-Human fumarate hydratase (FH), nuclear gene encoding mitochondrial protein |
CNY 5,704.00 |
