Superoxide Dismutase 1 (SOD1) (NM_000454) Human Tagged ORF Clone
CAT#: RC200725
- TrueORF®
SOD1 (Myc-DDK-tagged)-Human superoxide dismutase 1, soluble (SOD1)
ORF Plasmid: tGFP
"NM_000454" in other vectors (6)
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CNY 1,800.00
CNY 3,705.00
Cited in 6 publications. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; STAHP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200725 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGAAGGCCGTGTGCGTGCTGAAGGGCGACGGCCCAGTGCAGGGCATCATCAATTTCGAGCAGA AGGAAAGTAATGGACCAGTGAAGGTGTGGGGAAGCATTAAAGGACTGACTGAAGGCCTGCATGGATTCCA TGTTCATGAGTTTGGAGATAATACAGCAGGCTGTACCAGTGCAGGTCCTCACTTTAATCCTCTATCCAGA AAACACGGTGGGCCAAAGGATGAAGAGAGGCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAAGATG GTGTGGCCGATGTGTCTATTGAAGATTCTGTGATCTCACTCTCAGGAGACCATTGCATCATTGGCCGCAC ACTGGTGGTCCATGAAAAAGCAGATGACTTGGGCAAAGGTGGAAATGAAGAAAGTACAAAGACAGGAAAC GCTGGAAGTCGTTTGGCTTGTGGTGTAATTGGGATCGCCCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200725 protein sequence
Red=Cloning site Green=Tags(s) MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSR KHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGN AGSRLACGVIGIAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000454 |
ORF Size | 462 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000454.5 |
RefSeq Size | 981 bp |
RefSeq ORF | 465 bp |
Locus ID | 6647 |
UniProt ID | P00441 |
Domains | sodcu |
Protein Families | Druggable Genome |
Protein Pathways | Amyotrophic lateral sclerosis (ALS), Huntington's disease, Prion diseases |
MW | 15.9 kDa |
Gene Summary | The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2020] |
Citations (6)
The use of this cDNA Clones has been cited in the following citations: |
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A potential role for neuronal connexin 36 in the pathogenesis of amyotrophic lateral sclerosis
,Belousov, AB;Nishimune, H;Denisova, JV;Fontes, JD;,
Neurosci. Lett.
,PubMed ID 29246791
[Superoxide Dismutase 1]
|
M2 polarization of macrophages facilitates arsenic-induced cell transformation of lung epithelial cells
,Cui, J;Xu, W;Chen, J;Li, H;Dai, L;Frank, JA;Peng, S;Wang, S;Chen, G;,
Oncotarget
,PubMed ID 28423485
[Superoxide Dismutase 1]
|
Superoxide Dismutase 1 Regulation of CXCR4-Mediated Signaling in Prostate Cancer Cells is Dependent on Cellular Oxidative State
,Young, B;Purcell, C;Kuang, YQ;Charette, N;Dupré, DJ;,
Cell. Physiol. Biochem.
,PubMed ID 26599430
[Superoxide Dismutase 1]
|
Cadmium induces carcinogenesis in BEAS-2B cells through ROS-dependent activation of PI3K/AKT/GSK-3?/?-catenin signaling
,null,
Toxicology and applied pharmacology
,PubMed ID 22884995
[Superoxide Dismutase 1]
|
Superoxide dismutase 1 (SOD1) is a target for a small molecule identified in a screen for inhibitors of the growth of lung adenocarcinoma cell lines
,Romel Somwar, Hediye Erdjument-Bromage, Erik Larsson, David Shum, William W. Lockwood, Guangli Yang, Chris Sander, Ouathek Ouerfelli, Paul J. Tempst, Hakim Djaballah, and Harold E. Varmus,
PNAS, Sep 2011; 108: 16375 - 16380.
[Superoxide Dismutase 1]
|
The Dual Roles of c-Jun NH2-Terminal Kinase Signaling in Cr(VI)-Induced Apoptosis in JB6 Cells
,Young-Ok Son, John Andrew Hitron, Senping Cheng, Amit Budhraja, Zhuo Zhang, Nancy Lan Guo, Jeong-Chae Lee, and Xianglin Shi,
Toxicol. Sci., Feb 2011; 119: 335 - 345
[Superoxide Dismutase 1]
|
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200725L1 | Lenti ORF clone of Human superoxide dismutase 1, soluble (SOD1), Myc-DDK-tagged |
CNY 4,200.00 |
|
RC200725L2 | Lenti ORF clone of Human superoxide dismutase 1, soluble (SOD1), mGFP tagged |
CNY 5,890.00 |
|
RC200725L3 | Lenti ORF clone of Human superoxide dismutase 1, soluble (SOD1), Myc-DDK-tagged |
CNY 4,200.00 |
|
RC200725L4 | Lenti ORF clone of Human superoxide dismutase 1, soluble (SOD1), mGFP tagged |
CNY 4,200.00 |
|
RG200725 | SOD1 (tGFP-tagged) - Human superoxide dismutase 1, soluble (SOD1) |
CNY 3,400.00 |
|
SC111022 | SOD1 (untagged)-Human superoxide dismutase 1, soluble (SOD1) |
CNY 1,200.00 |
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