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Superoxide Dismutase 1 (SOD1) (NM_000454) Human Untagged Clone
CAT＃: SC111022
SOD1 (untagged)-Human superoxide dismutase 1, soluble (SOD1)
CNY 1,200.00
CNY 2,950.00
| Cited in 5 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; STAHP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111022 sequence for NM_000454 edited (data generated by NextGen Sequencing)
ATGGCGACGAAGGCCGTGTGCGTGCTGAAGGGCGACGGCCCAGTGCAGGGCATCATCAAT TTCGAGCAGAAGGAAAGTAATGGACCAGTGAAGGTGTGGGGAAGCATTAAAGGACTGACT GAAGGCCTGCATGGATTCCATGTTCATGAGTTTGGAGATAATACAGCAGGCTGTACCAGT GCAGGTCCTCACTTTAATCCTCTATCCAGAAAACACGGTGGGCCAAAGGATGAAGAGAGG CATGTTGGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTCTATT GAAGATTCTGTGATCTCACTCTCAGGAGACCATTGCATCATTGGCCGCACACTGGTGGTC CATGAAAAAGCAGATGACTTGGGCAAAGGTGGAAATGAAGAAAGTACAAAGACAGGAAAC GCTGGAAGTCGTTTGGCTTGTGGTGTAATTGGGATCGCCCAATAA Clone variation with respect to NM_000454.4 >OriGene 5' read for NM_000454 unedited
ACCGGTTTTGAAACCGACTCATATAGGCGGCAACCAATTCGCACGAGGTGCTGGTTTGCG TCGTAGTCTCCTGCAGCGTCTGGGGTTTCCGTTGCAGTCCTCGGAACCAGGACCTCGGCG TGGCCTATCGAGTTATGGCGACGAAGGCCGTGTGCGTGCTGAAGGGCGACGGCCCAGTGC AGGGCATCATCAATTTCGAGCAGAATGTTTTTTATGGACCAGTGAAGGTGTGGGGAAGCA TTAAAGGACTGACTGAAGGCCTGCATGGATTCCATGTTCATGAGTTTGGAGATAATACAG CAGGCTGTACCAGTGCAGGTCCTCACTTTAATCCTCTATCCAGAAAACACGGTGGGCCAA AGGATGAAGAGAGGCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGG CCGATGTGTCTATTGAAGATTCTGTGATCTCACTCTCAGGAGACCATTGCATCATTGGCC GCACACTGGTGGTCCATGAAAAAGCAGATGACTTGGGCAAAGGTGGAAATGAAGAAAGTA CAAAGACAGGAAACGCTGGAAGTCGTTTGGCTTGTGGTGTAATTGGGATCGCCCAATAAA CATTCCCTTGGATGTAGTCTGAGGCCCCTTAACTCATCTGTTATCCTGCTAGCTGTAGAA ATGTATCCTGATAAACATTAAACACTGTAATCTTAAAAGTGTAATTGTGTGACTTTTTCA GAGTTGCTTTAAAGTACCTGTAGTGAGAAACTGATTTATGATCACTTGGAAGATCTGTAT AGNTTTATAAAACTCAGTTAAAATGTCTGTTTCAATGACCTGTATTTTGCCACGACTTAA TCACAGATGGGTATTAAACCTGTCAGAATTTCTTGTCATTCAAGCCTTGAATAAAAAACC TGTATGGCACTTTTAA >OriGene 3' read for NM_000454 unedited
GTCTGCCCGCGGCCGCATCTAGGTCGGCTTTTTTTTTTTTTTTTTGAATTTGGATTCTTT TAATAGCCTCATAATAAGTGCCATACAGGGTTTTTATTCACAGGCTTGAATGACAAAGAA ATTCTGACAAGTTTAATACCCATCTGTGATTTAAGTCTGGCAAAATACAGGTCATTGAAA CAGACATTTTAACTGAGTTTTATAAAACTATACAAATCTTCCAAGTGATCATAAATCAGT TTCTCACTACAGGTACTTTAAAGCAACTCTGAAAAAGTCACACAATTACACTTTTAAGAT TACAGTGTTTAATGTTTATCAGGATACATTTCTACAGCTAGCAGGATAACAGATGAGTTA AGGGGCCTCAGACTACCTCCACGGGAATGTTTATCGGGCGATCCCAATTACACCACAAGC CAAACGACTTCCAGCGTTTCCTGTCTTTGCACTTTCTTCATTTTCACCTTTGCCCAAGTC ATCTGCTTTTTCATGGACCACCAGTGTGCGGCCAATGATGCAATGGTCTCCTGAGAGTGA GATCACAGAATCTTCACTAGACACATCGGCCACACCATCTTTGTCAGCAGCCACATTGCC CAAGTCTCCAACATGCCTCTNTTTATCCTTTGGCCCACCGTGTTTTCTGGATAGAGGATC AAAGTGATGACCCTGTCCTGTCACCGCCTGCTGTATTATCTCCAAACTCATGAACATGGC ATCCACTGCGGCCCTAACTCACCTCTTTAATGCTTCCCCAACCCTTACTGGCCATTCACT TCCTTTTTGCTCGACAATGATGAATGCCCTGCCCTGAGCCCGTCGTCCTTTTTCCCCCCC AACGCACTTTGTTCCCCTCACCTCCGTTTAGCCCCGCCCCAGTTCCTGGCTTCCAGGGAC TGCAACCGAAACCCCACAACCCTGGAGGAGAATACGAGCCAAACCATATACCGCCGCGCG CATTCCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000454 |
| Insert Size | 940 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000454.4, NP_000445.1 |
| RefSeq Size | 981 bp |
| RefSeq ORF | 465 bp |
| Locus ID | 6647 |
| UniProt ID | P00441 |
| Domains | sodcu |
| Protein Families | Druggable Genome |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Huntington's disease, Prion diseases |
| Gene Summary | The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2020] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Glycation in Demetalated Superoxide Dismutase 1 Prevents Amyloid Aggregation and Produces Cytotoxic Ages Adducts
,Sirangelo, I;Vella, FM;Irace, G;Manco, G;Iannuzzi, C;,
Front Mol Biosci
,PubMed ID 27695694
[SOD1]
|
|
Nanoluciferase signal brightness using furimazine substrates opens bioluminescence resonance energy transfer to widefield microscopy
,Kim, J;Grailhe, R;,
Cytometry A
,PubMed ID 27144967
[SOD1]
|
|
Dimerization, oligomerization, and aggregation of human Amyotrophic lateral sclerosis Cu/Zn-superoxide dismutase 1 mutant forms in live cells
,Kim, J;Lee, H;Lee, JH;Kwon, DY;Genovesio, A;Fenistein, D;Ogier, A;Brondani, V;Grailhe, R;,
J. Biol. Chem. April 2014
,PubMed ID 24692554
[SOD1]
|
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NADPH Oxidase Activation Is Required in Reactive Oxygen Species Generation and Cell Transformation Induced by Hexavalent Chromium
,Xin Wang, Young-Ok Son, Qingshan Chang, Lijuan Sun, J.Andrew Hitron, Amit Budhraja, Zhuo Zhang, Zunji Ke, Fei Chen, Jia Luo, and Xianglin Shi,
Toxicol. Sci., Oct 2011; 123: 399 - 410.
[SOD1]
|
|
Proteomic Analysis Identifies Oxidative Stress Induction by Adaphostin
,Luke H Stockwin, Maja A Bumke, Sherry X Yu, Simon P Webb, Jack R Collins, Melinda G Hollingshead, and Dianne L Newton,
Clin Cancer Res. 2007 Jun 15;13(12):3667-3681
[SOD1]
|
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Resources
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