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TMEM70 (NM_017866) Human Tagged ORF Clone
CAT＃: RC201060
TMEM70 (Myc-DDK-tagged)-Human transmembrane protein 70 (TMEM70), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid: tGFP
"NM_017866" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MC5DN2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201060 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTTTCTGGCGTTGGGCAGCCCGTGGGCGGTCGAACTGCCTCTCTGCGGAAGGAGGACTGCATTGT GTGCGGCCGCCGCGCTCCGAGGTCCCCGGGCCTCTGTCTCCCGGGCGTCCTCCAGCAGCGGGCCTTCGGG GCCGGTAGCCGGCTGGAGTACGGGGCCTTCGGGAGCCGCGCGCCTTCTCCGGCGTCCGGGTCGAGCGCAG ATCCCTGTTTATTGGGAAGGATATGTTCGATTCTTAAATACGCCATCTGACAAATCAGAAGATGGAAGGC TAATTTATACTGGCAATATGGCCCGAGCAGTGTTTGGTGTGAAATGTTTCTCTTATTCTACGAGTCTGAT TGGCCTTACATTTCTGCCATACATTTTTACACAAAATAATGCTATTTCTGAAAGTGTGCCTCTGCCTATT CAAATCATATTCTATGGCATCATGGGAAGCTTTACGGTGATCACCCCAGTGCTGCTTCACTTTATTACAA AAGGCTATGTCATTCGATTGTACCATGAGGCCACAACAGACACTTATAAAGCCATTACCTACAATGCTAT GCTTGCAGAAACGAGTACAGTGTTTCACCAGAATGATGTGAAGATTCCAGATGCTAAACATGTATTTACC ACATTTTATGCTAAAACAAAATCACTGTTAGTTAATCCAGTGCTCTTTCCAAACCGTGAAGACTATATCC ATCTAATGGGTTATGACAAAGAAGAATTTATTTTGTATATGGAAGAAACCAGTGAAGAGAAACGGCATAA AGATGACAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201060 protein sequence
Red=Cloning site Green=Tags(s) MLFLALGSPWAVELPLCGRRTALCAAAALRGPRASVSRASSSSGPSGPVAGWSTGPSGAARLLRRPGRAQ IPVYWEGYVRFLNTPSDKSEDGRLIYTGNMARAVFGVKCFSYSTSLIGLTFLPYIFTQNNAISESVPLPI QIIFYGIMGSFTVITPVLLHFITKGYVIRLYHEATTDTYKAITYNAMLAETSTVFHQNDVKIPDAKHVFT TFYAKTKSLLVNPVLFPNREDYIHLMGYDKEEFILYMEETSEEKRHKDDK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017866 |
| ORF Size | 780 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017866.6 |
| RefSeq Size | 2096 bp |
| RefSeq ORF | 783 bp |
| Locus ID | 54968 |
| UniProt ID | Q9BUB7 |
| Protein Families | Transmembrane |
| MW | 29 kDa |
| Gene Summary | This gene likely encodes a mitochondrial membrane protein. The encoded protein may play a role in biogenesis of mitochondrial ATP synthase. Mutations in this gene have been associated with neonatal mitochondrial encephalocardiomyopathy due to ATP synthase deficiency. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mitochondrial membrane assembly of TMEM70 protein
,KratochvÃlová, H;Hejzlarová, K;Vrbacký, M;MráÄek, T;Karbanová, V;TesaÅ™ová, M;Gombitová, A;Cmarko, D;Wittig, I;Zeman, J;HouÅ¡tÄ›k, J;,
Mitochondrion, Feb 2014.
,PubMed ID 24576557
[TMEM70]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201060L3 | Lenti ORF clone of Human transmembrane protein 70 (TMEM70), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201060L4 | Lenti ORF clone of Human transmembrane protein 70 (TMEM70), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201060 | TMEM70 (tGFP-tagged) - Human transmembrane protein 70 (TMEM70), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,000.00 |
|
| SC113906 | TMEM70 (untagged)-Human transmembrane protein 70 (TMEM70), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2,400.00 |
