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NSE (ENO2) (NM_001975) Human Tagged ORF Clone
CAT＃: RC201085
ENO2 (Myc-DDK-tagged)-Human enolase 2 (gamma, neuronal) (ENO2)
ORF Plasmid: tGFP
"NM_001975" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL-S-279; NSE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201085 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCATAGAGAAGATCTGGGCCCGGGAGATCCTGGACTCCCGCGGGAACCCCACAGTGGAGGTGGATC TCTATACTGCCAAAGGTCTTTTCCGGGCTGCAGTGCCCAGTGGAGCCTCTACGGGCATCTATGAGGCCCT GGAGCTGAGGGATGGAGACAAACAGCGTTACTTAGGCAAAGGTGTCCTGAAGGCAGTGGACCACATCAAC TCCACCATCGCGCCAGCCCTCATCAGCTCAGGTCTCTCTGTGGTGGAGCAAGAGAAACTGGACAACCTGA TGCTGGAGTTGGATGGGACTGAGAACAAATCCAAGTTTGGGGCCAATGCCATCCTGGGTGTGTCTCTGGC CGTGTGTAAGGCAGGGGCAGCTGAGCGGGAACTGCCCCTGTATCGCCACATTGCTCAGCTGGCCGGGAAC TCAGACCTCATCCTGCCTGTGCCGGCCTTCAACGTGATCAATGGTGGCTCTCATGCTGGCAACAAGCTGG CCATGCAGGAGTTCATGATCCTCCCAGTGGGAGCTGAGAGCTTTCGGGATGCCATGCGACTAGGTGCAGA GGTCTACCATACACTCAAGGGAGTCATCAAGGACAAATACGGCAAGGATGCCACCAATGTGGGGGATGAA GGTGGCTTTGCCCCCAATATCCTGGAGAACAGTGAAGCCTTGGAGCTGGTGAAGGAAGCCATCGACAAGG CTGGCTACACGGAAAAGATCGTTATTGGCATGGATGTTGCTGCCTCAGAGTTTTATCGTGATGGCAAATA TGACTTGGACTTCAAGTCTCCCACTGATCCTTCCCGATACATCACTGGGGACCAGCTGGGGGCACTCTAC CAGGACTTTGTCAGGGACTATCCTGTGGTCTCCATTGAGGACCCATTTGACCAGGATGATTGGGCTGCCT GGTCCAAGTTCACAGCCAATGTAGGGATCCAGATTGTGGGTGATGACCTGACAGTGACCAACCCAAAACG TATTGAGCGGGCAGTGGAAGAAAAGGCCTGCAACTGTCTGCTGCTCAAGGTCAACCAGATCGGCTCTGTC ACTGAAGCCATCCAAGCGTGCAAGCTGGCCCAGGAGAATGGCTGGGGGGTCATGGTGAGTCATCGCTCAG GAGAGACTGAGGACACATTCATTGCTGACCTGGTGGTGGGGCTGTGCACAGGCCAGATCAAGACTGGTGC CCCGTGCCGTTCTGAACGTCTGGCTAAATACAACCAGCTCATGAGAATTGAGGAAGAGCTGGGGGATGAA GCTCGCTTTGCCGGACATAACTTCCGTAATCCCAGTGTGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201085 protein sequence
Red=Cloning site Green=Tags(s) MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHIN STIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGN SDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDE GGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALY QDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSV TEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDE ARFAGHNFRNPSVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001975 |
| ORF Size | 1302 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001975.3 |
| RefSeq Size | 2423 bp |
| RefSeq ORF | 1305 bp |
| Locus ID | 2026 |
| UniProt ID | P09104 |
| Domains | enolase |
| Protein Pathways | Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation |
| MW | 47.3 kDa |
| Gene Summary | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201085L1 | Lenti ORF clone of Human enolase 2 (gamma, neuronal) (ENO2), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201085L2 | Lenti ORF clone of Human enolase 2 (gamma, neuronal) (ENO2), mGFP tagged |
CNY 5,890.00 |
|
| RC201085L3 | Lenti ORF clone of Human enolase 2 (gamma, neuronal) (ENO2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201085L4 | Lenti ORF clone of Human enolase 2 (gamma, neuronal) (ENO2), mGFP tagged |
CNY 5,890.00 |
|
| RG201085 | ENO2 (tGFP-tagged) - Human enolase 2 (gamma, neuronal) (ENO2) |
CNY 5,256.00 |
|
| SC319063 | ENO2 (untagged)-Human enolase 2 (gamma, neuronal) (ENO2) |
CNY 3,656.00 |
