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PPAP2C (PLPP2) (NM_003712) Human Tagged ORF Clone
CAT＃: RC201192
PPAP2C (Myc-DDK-tagged)-Human phosphatidic acid phosphatase type 2C (PPAP2C), transcript variant 1
ORF Plasmid: tGFP
"NM_003712" in other vectors (5)
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CNY 2,400.00
CNY 4,465.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LPP2; PAP-2c; PAP2-g; PPAP2C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201192 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCGGAGGTGGGTCTTCGTGCTGCTCGACGTGCTGTGCTTACTGGTCGCCTCCCTGCCCTTCGCTA TCCTGACGCTGGTGAACGCCCCGTACAAGCGAGGATTTTACTGCGGGGATGACTCCATCCGGTACCCCTA CCGTCCAGATACCATCACCCACGGGCTCATGGCTGGGGTCACCATCACGGCCACCGTCATCCTTGTCTCG GCCGGGGAAGCCTACCTGGTGTACACAGACCGGCTCTATTCTCGCTCGGACTTCAACAACTACGTGGCTG CTGTATACAAGGTGCTGGGGACCTTCCTGTTTGGGGCTGCCGTGAGCCAGTCTCTGACAGACCTGGCCAA GTACATGATTGGGCGTCTGAGGCCCAACTTCCTAGCCGTCTGCGACCCCGACTGGAGCCGGGTCAACTGC TCGGTCTATGTGCAGCTGGAGAAGGTGTGCAGGGGAAACCCTGCTGATGTCACCGAGGCCAGGTTGTCTT TCTACTCGGGACACTCTTCCTTTGGGATGTACTGCATGGTGTTCTTGGCGCTGTATGTGCAGGCACGACT CTGTTGGAAGTGGGCACGGCTGCTGCGACCCACAGTCCAGTTCTTCCTGGTGGCCTTTGCCCTCTACGTG GGCTACACCCGCGTGTCTGATTACAAACACCACTGGAGCGATGTCCTTGTTGGCCTCCTGCAGGGGGCAC TGGTGGCTGCCCTCACTGTCTGCTACATCTCAGACTTCTTCAAAGCCCGACCCCCACAGCACTGTCTGAA GGAGGAGGAGCTGGAACGGAAGCCCAGCCTGTCACTGACGTTGACCCTGGGCGAGGCTGACCACAACCAC TATGGATACCCGCACTCCTCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201192 protein sequence
Red=Cloning site Green=Tags(s) MQRRWVFVLLDVLCLLVASLPFAILTLVNAPYKRGFYCGDDSIRYPYRPDTITHGLMAGVTITATVILVS AGEAYLVYTDRLYSRSDFNNYVAAVYKVLGTFLFGAAVSQSLTDLAKYMIGRLRPNFLAVCDPDWSRVNC SVYVQLEKVCRGNPADVTEARLSFYSGHSSFGMYCMVFLALYVQARLCWKWARLLRPTVQFFLVAFALYV GYTRVSDYKHHWSDVLVGLLQGALVAALTVCYISDFFKARPPQHCLKEEELERKPSLSLTLTLGEADHNH YGYPHSSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003712 |
| ORF Size | 864 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003712.4 |
| RefSeq Size | 1320 bp |
| RefSeq ORF | 867 bp |
| Locus ID | 8612 |
| UniProt ID | O43688 |
| Domains | acidPPc |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transmembrane |
| Protein Pathways | Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Sphingolipid metabolism |
| MW | 32.6 kDa |
| Gene Summary | The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is similar to phosphatidic acid phosphatase type 2A (PPAP2A) and type 2B (PPAP2B). All three proteins contain 6 transmembrane regions, and a consensus N-glycosylation site. This protein has been shown to possess membrane associated PAP activity. Three alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201192L3 | Lenti ORF clone of Human phosphatidic acid phosphatase type 2C (PPAP2C), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201192L4 | Lenti ORF clone of Human phosphatidic acid phosphatase type 2C (PPAP2C), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201192 | PPAP2C (tGFP-tagged) - Human phosphatidic acid phosphatase type 2C (PPAP2C), transcript variant 1 |
CNY 4,370.00 |
|
| SC109963 | PPAP2C (untagged)-Human phosphatidic acid phosphatase type 2C (PPAP2C), transcript variant 1 |
CNY 2,400.00 |
|
| SC320713 | PPAP2C (untagged)-Human phosphatidic acid phosphatase type 2C (PPAP2C), transcript variant 1 |
CNY 2,400.00 |
