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Cip4 (TRIP10) (NM_004240) Human Tagged ORF Clone
CAT＃: RC201200
TRIP10 (Myc-DDK-tagged)-Human thyroid hormone receptor interactor 10 (TRIP10)
ORF Plasmid: tGFP
"NM_004240" in other vectors (4)
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CNY 4,056.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CIP4; HSTP; STOT; STP; TRIP-10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201200 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTGGGGCACTGAGCTGTGGGATCAGTTCGAGGTGCTCGAGCGCCACACGCAGTGGGGGCTGGACC TGTTGGACAGATATGTAAAGTTCGTGAAAGAACGCACCGAAGTGGAACAGGCTTACGCCAAACAACTGCG GAGCCTGGTGAAAAAATATCTGCCCAAGAGACCTGCCAAGGATGATCCTGAGTCCAAATTCAGCCAGCAA CAGTCCTTCGTACAGATTCTCCAGGAGGTGAATGACTTTGCAGGCCAGCGGGAGCTGGTGGCTGAGAACC TCAGTGTCCGTGTATGTCTTGAGCTGACCAAGTACTCACAAGAGATGAAACAGGAGAGGAAGATGCACTT CCAAGAAGGGCGGCGGGCCCAGCAGCAGCTGGAAAATGGCTTTAAACAGCTGGAGAATAGTAAGCGTAAA TTTGAGCGGGACTGCCGGGAGGCAGAGAAGGCAGCCCAGACTGCTGAACGGCTAGACCAGGATATCAACG CCACCAAGGCTGATGTGGAGAAGGCCAAGCAGCAAGCCCACCTTCGGAGTCACATGGCCGAAGAAAGCAA AAACGAATATGCGGCTCAACTGCAGCGCTTCAACCGAGACCAAGCCCACTTCTATTTTTCACAGATGCCC CAGATATTCGATAAGCTCCAAGACATGGATGAACGCAGGGCCACCCGCCTGGGTGCCGGGTATGGGCTCC TGTCGGAGGCCGAGCTGGAGGTGGTGCCCATAATAGCCAAGTGCTTGGAGGGCATGAAGGTGGCTGCAAA TGCTGTGGATCCCAAGAACGACTCCCACGTCCTTATAGAGCTGCACAAGTCAGGTTTTGCCCGCCCGGGC GACGTGGAATTCGAGGACTTCAGCCAGCCCATGAACCGTGCACCCTCCGACAGCAGTCTGGGCACCCCCT CGGATGGACGGCCTGAACTCCGAGGCCCGGGTCGCAGCCGCACCAAGCGCTGGCCTTTTGGCAAGAAGAA CAAGACAGTGGTGACCGAGGATTTTAGCCACTTGCCCCCAGAGCAGCAGCGAAAACGGCTTCAACAGCAG TTGGAAGAACGCAGTCGTGAACTTCAGAAGGAGGTTGACCAGAGGGAAGCCCTAAAGAAAATGAAGGATG TCTATGAGAAGACACCTCAGATGGGGGACCCCGCCAGCTTGGAGCCCCAGATCGCTGAAACCCTGAGCAA CATTGAACGGCTGAAATTGGAAGTGCAGAAGTATGAGGCGTGGCTGGCAGAAGCTGAAAGTCGAGTCCTT AGCAACCGGGGAGACAGCCTGAGCCGGCACGCCCGGCCTCCCGACCCCCCCGCTAGCGCCCCGCCAGACA GCAGCAGCAACAGCGCATCACAGGACACCAAGGAGAGCTCTGAAGAGCCTCCCTCAGAAGAGAGCCAGGA CACCCCCATTTACACGGAGTTTGATGAGGATTTCGAGGAGGAACCCACATCCCCCATAGGTCACTGTGTG GCCATCTACCACTTTGAAGGGTCCAGCGAGGGCACTATCTCTATGGCCGAGGGTGAAGACCTCAGTCTTA TGGAAGAAGACAAAGGGGACGGCTGGACCCGGGTCAGGCGGAAAGAGGGAGGCGAGGGCTACGTGCCCAC CTCCTACCTCCGAGTCACGCTCAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201200 protein sequence
Red=Cloning site Green=Tags(s) MDWGTELWDQFEVLERHTQWGLDLLDRYVKFVKERTEVEQAYAKQLRSLVKKYLPKRPAKDDPESKFSQQ QSFVQILQEVNDFAGQRELVAENLSVRVCLELTKYSQEMKQERKMHFQEGRRAQQQLENGFKQLENSKRK FERDCREAEKAAQTAERLDQDINATKADVEKAKQQAHLRSHMAEESKNEYAAQLQRFNRDQAHFYFSQMP QIFDKLQDMDERRATRLGAGYGLLSEAELEVVPIIAKCLEGMKVAANAVDPKNDSHVLIELHKSGFARPG DVEFEDFSQPMNRAPSDSSLGTPSDGRPELRGPGRSRTKRWPFGKKNKTVVTEDFSHLPPEQQRKRLQQQ LEERSRELQKEVDQREALKKMKDVYEKTPQMGDPASLEPQIAETLSNIERLKLEVQKYEAWLAEAESRVL SNRGDSLSRHARPPDPPASAPPDSSSNSASQDTKESSEEPPSEESQDTPIYTEFDEDFEEEPTSPIGHCV AIYHFEGSSEGTISMAEGEDLSLMEEDKGDGWTRVRRKEGGEGYVPTSYLRVTLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004240 |
| ORF Size | 1635 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004240.4 |
| RefSeq Size | 2033 bp |
| RefSeq ORF | 1638 bp |
| Locus ID | 9322 |
| UniProt ID | Q15642 |
| Protein Families | Druggable Genome |
| Protein Pathways | Insulin signaling pathway |
| MW | 62.6 kDa |
| Gene Summary | Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery
,Chang, TY;Chen, C;Lee, M;Chang, YC;Lu, CH;Lu, ST;Wang, DY;Wang, A;Guo, CL;Cheng, PL;,
Elife
,PubMed ID 29271742
[Cip4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201200L3 | Lenti ORF clone of Human thyroid hormone receptor interactor 10 (TRIP10), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201200L4 | Lenti ORF clone of Human thyroid hormone receptor interactor 10 (TRIP10), mGFP tagged |
CNY 5,890.00 |
|
| RG201200 | TRIP10 (tGFP-tagged) - Human thyroid hormone receptor interactor 10 (TRIP10) |
CNY 4,370.00 |
|
| SC122672 | TRIP10 (untagged)-Human thyroid hormone receptor interactor 10 (TRIP10) |
CNY 4,064.00 |
