Prohibitin (PHB) (NM_002634) Human Tagged ORF Clone

Name: Prohibitin (PHB) (NM_002634) Human Tagged ORF Clone
SKU: RC201229
Price: 2400.00

CAT＃: RC201229

PHB (Myc-DDK-tagged)-Human prohibitin (PHB)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_002634" in other vectors (6)

Need custom modification / cloning service?
Get a free quote

CNY 2,400.00

CNY 4,465.00

货期*

现货

规格

Cited in 5 publications.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA349430

Rabbit Polyclonal Anti-PHB Antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HEL-215; HEL-S-54e; PHB1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC201229 representing NM_002634 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCAAAGTGTTTGAGTCCATTGGCAAGTTTGGCCTGGCCTTAGCTGTTGCAGGAGGCGTGGTGA ACTCTGCCTTATATAATGTGGATGCTGGGCACAGAGCTGTCATCTTTGACCGATTCCGTGGAGTGCAGGA CATTGTGGTAGGGGAAGGGACTCATTTTCTCATCCCGTGGGTACAGAAACCAATTATCTTTGACTGCCGT TCTCGACCACGTAATGTGCCAGTCATCACTGGTAGCAAAGATTTACAGAATGTCAACATCACACTGCGCA TCCTCTTCCGGCCTGTCGCCAGCCAGCTTCCTCGCATCTTCACCAGCATCGGAGAGGACTATGATGAGCG TGTGCTGCCGTCCATCACAACTGAGATCCTCAAGTCAGTGGTGGCTCGCTTTGATGCTGGAGAACTAATC ACCCAGAGAGAGCTGGTCTCCAGGCAGGTGAGCGACGACCTTACAGAGCGAGCCGCCACCTTTGGGCTCA TCCTGGATGACGTGTCCTTGACACATCTGACCTTCGGGAAGGAGTTCACAGAAGCGGTGGAAGCCAAACA GGTGGCTCAGCAGGAAGCAGAGAGGGCCAGATTTGTGGTGGAAAAGGCTGAGCAACAGAAAAAGGCGGCC ATCATCTCTGCTGAGGGCGACTCCAAGGCAGCTGAGCTGATTGCCAACTCACTGGCCACTGCAGGGGATG GCCTGATCGAGCTGCGCAAGCTGGAAGCTGCAGAGGACATCGCGTACCAGCTCTCACGCTCTCGGAACAT CACCTACCTGCCAGCGGGGCAGTCCGTGCTCCTCCAGCTGCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC201229 representing NM_002634 Red=Cloning site Green=Tags(s) MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCR SRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELI TQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAA IISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_002634
ORF Size	816 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_002634.4
RefSeq Size	1826 bp
RefSeq ORF	819 bp
Locus ID	5245
UniProt ID	P35232
Domains	Band_7
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
MW	29.6 kDa
Gene Summary	This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (5)

The use of this cDNA Clones has been cited in the following citations:
GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer\'s disease ,Xu, P;Chang, JC;Zhou, X;Wang, W;Bamkole, M;Wong, E;Bettayeb, K;Jiang, LL;Huang, T;Luo, W;Xu, H;Nairn, AC;Flajolet, M;Ip, NY;Li, YM;Greengard, P;, The Journal of experimental medicine ,PubMed ID 34156424 [Prohibitin]
The mitochondrial chaperone Prohibitin 1 negatively regulates interleukin-8 in human liver cancers ,Yang, JW;Murray, B;Barbier-Torres, L;Liu, T;Liu, Z;Yang, H;Fan, W;Wang, J;Li, Y;Seki, E;Mato, JM;Lu, SC;, J. Biol. Chem. ,PubMed ID 30523154 [Prohibitin]
Prohibitin plays a critical role in Enterovirus 71 neuropathogenesis ,Too, IHK;Bonne, I;Tan, EL;Chu, JJH;Alonso, S;, PLoS Pathog. ,PubMed ID 29324904 [Prohibitin]
Prohibitin 1 suppresses liver cancer tumorigenesis in mice and human hepatocellular and cholangiocarcinoma cells ,Fan, W;Yang, H;Liu, T;Wang, J;Li, TW;Mavila, N;Tang, Y;Yang, J;Peng, H;Tu, J;Annamalai, A;Noureddin, M;Krishnan, A;Gores, GJ;Martínez-Chantar, ML;Mato, JM;Lu, SC;, Hepatology ,PubMed ID 27981602 [Prohibitin]
Prohibitin Overexpression in Adipocytes Induces Mitochondrial Biogenesis, Leads to Obesity Development, and Affects Glucose Homeostasis in a Sex-Specific Manner ,Ande, SR;Nguyen, KH;Padilla-Meier, GP;Wahida, W;Nyomba, BL;Mishra, S;, Diabetes ,PubMed ID 24947361 [Prohibitin]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC201229L1	Lenti ORF clone of Human prohibitin (PHB), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC201229L2	Lenti ORF clone of Human prohibitin (PHB), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC201229L3	Lenti ORF clone of Human prohibitin (PHB), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00 CNY 5,890.00
RC201229L4	Lenti ORF clone of Human prohibitin (PHB), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,800.00
RG201229	PHB (tGFP-tagged) - Human prohibitin (PHB)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,000.00 CNY 4,370.00
SC110973	PHB (untagged)-Human prohibitin (PHB)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 2,950.00