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DRG1 (NM_004147) Human Tagged ORF Clone
CAT＃: RC201259
DRG1 (Myc-DDK-tagged)-Human developmentally regulated GTP binding protein 1 (DRG1)
ORF Plasmid: tGFP
"NM_004147" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NEDD3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201259 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCACCTTAGCTAAGATCGCGGAGATAGAAGCAGAGATGGCTCGGACTCAAAAGAACAAGGCCA CAGCACACCACTTAGGGCTGCTTAAGGCTCGTCTTGCTAAGCTTCGTCGAGAACTCATTACTCCAAAGGG TGGTGGTGGTGGAGGTCCAGGAGAAGGTTTTGATGTGGCCAAGACAGGTGATGCTCGAATTGGATTTGTT GGTTTTCCATCTGTGGGGAAGTCAACACTGCTTAGTAACCTGGCAGGGGTATATTCTGAGGTGGCAGCCT ATGAATTCACTACTCTGACCACTGTGCCTGGTGTCATCAGATACAAAGGTGCCAAGATCCAGCTCCTGGA TCTCCCAGGTATCATTGAAGGTGCCAAGGATGGGAAAGGTAGAGGTCGTCAAGTCATTGCAGTGGCCCGA ACCTGTAACTTGATCTTGATTGTTCTGGATGTCCTGAAACCTTTGGGACATAAGAAGATAATTGAAAATG AGCTGGAAGGCTTTGGCATTCGCTTGAACAGCAAACCCCCCAACATTGGCTTTAAGAAGAAGGACAAGGG AGGCATTAATCTCACAGCCACTTGCCCCCAGAGTGAGCTGGATGCTGAAACTGTGAAGAGCATTCTGGCT GAATACAAGATTCATAATGCCGATGTGACTCTACGTAGTGATGCTACAGCTGATGACCTCATTGATGTGG TGGAAGGAAACAGAGTTTATATCCCCTGTATCTATGTGTTAAATAAGATTGACCAAATCTCCATTGAGGA ATTGGATATCATCTATAAGGTGCCTCACTGTGTACCCATCTCTGCCCATCACCGCTGGAATTTTGATGAC CTATTGGAAAAGATCTGGGACTATCTGAAACTAGTGAGAATTTACACCAAACCCAAAGGCCAGTTACCAG ATTACACATCCCCAGTGGTGCTTCCTTACTCCAGGACCACAGTGGAGGATTTCTGCATGAAGATTCACAA AAATCTTATCAAAGAATTTAAATATGCTCTGGTCTGGGGTCTCTCTGTGAAACACAATCCTCAGAAAGTG GGTAAAGACCATACGTTGGAGGATGAGGATGTCATTCAAATTGTGAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201259 protein sequence
Red=Cloning site Green=Tags(s) MSSTLAKIAEIEAEMARTQKNKATAHHLGLLKARLAKLRRELITPKGGGGGGPGEGFDVAKTGDARIGFV GFPSVGKSTLLSNLAGVYSEVAAYEFTTLTTVPGVIRYKGAKIQLLDLPGIIEGAKDGKGRGRQVIAVAR TCNLILIVLDVLKPLGHKKIIENELEGFGIRLNSKPPNIGFKKKDKGGINLTATCPQSELDAETVKSILA EYKIHNADVTLRSDATADDLIDVVEGNRVYIPCIYVLNKIDQISIEELDIIYKVPHCVPISAHHRWNFDD LLEKIWDYLKLVRIYTKPKGQLPDYTSPVVLPYSRTTVEDFCMKIHKNLIKEFKYALVWGLSVKHNPQKV GKDHTLEDEDVIQIVKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004147 |
| ORF Size | 1101 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004147.4 |
| RefSeq Size | 1467 bp |
| RefSeq ORF | 1104 bp |
| Locus ID | 4733 |
| UniProt ID | Q9Y295 |
| Domains | TGS, GTP1_OBG |
| Protein Families | Transcription Factors |
| MW | 40.5 kDa |
| Gene Summary | Catalyzes the conversion of GTP to GDP through hydrolysis of the gamma-phosphate bond in GTP (PubMed:29915238, PubMed:23711155). Appears to have an intrinsic GTPase activity that is stimulated by ZC3H15/DFRP1 binding likely by increasing the affinity for the potassium ions (PubMed:23711155). When hydroxylated at C-3 of 'Lys-22' by JMJD7, may bind to RNA and play a role in translation (PubMed:19819225, PubMed:29915238). Binds to microtubules and promotes microtubule polymerization and stability that are required for mitotic spindle assembly during prophase to anaphase transition. GTPase activity is not necessary for these microtubule-related functions (PubMed:28855639).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201259L3 | Lenti ORF clone of Human developmentally regulated GTP binding protein 1 (DRG1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201259L4 | Lenti ORF clone of Human developmentally regulated GTP binding protein 1 (DRG1), mGFP tagged |
CNY 5,890.00 |
|
| RG201259 | DRG1 (tGFP-tagged) - Human developmentally regulated GTP binding protein 1 (DRG1) |
CNY 5,256.00 |
|
| SC321213 | DRG1 (untagged)-Human developmentally regulated GTP binding protein 1 (DRG1) |
CNY 3,656.00 |
