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CoCoA (CALCOCO1) (NM_020898) Human Tagged ORF Clone
CAT＃: RC201375
CALCOCO1 (Myc-DDK-tagged)-Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1
ORF Plasmid: tGFP
"NM_020898" in other vectors (4)
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CNY 5,064.00
CNY 5,800.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | calphoglin; Cocoa; PP13275 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201375 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAATCACCACTAAGCCGGGCACCATCCCGTGGTGGAGTCAACTTTCTCAATGTAGCCCGGACCT ACATCCCCAACACCAAGGTGGAATGTCACTACACCCTTCCCCCAGGCACCATGCCCAGTGCCAGTGACTG GATTGGCATCTTCAAGGTGGAGGCTGCCTGTGTTCGGGATTACCACACATTTGTGTGGTCTTCCGTGCCT GAAAGTACAACTGATGGTTCCCCCATTCACACCAGTGTCCAGTTCCAAGCCAGCTACCTGCCCAAACCAG GAGCTCAGCTCTACCAGTTCCGATATGTGAACCGCCAGGGCCAGGTGTGTGGGCAGAGCCCCCCTTTCCA GTTCCGAGAGCCAAGGCCCATGGATGAACTGGTGACCCTGGAGGAGGCTGATGGGGGCTCTGACATCCTG CTGGTTGTCCCCAAGGCAACTGTGTTACAGAACCAGCTCGATGAGAGCCAGCAAGAACGGAATGACCTGA TGCAGCTGAAGCTACAGCTGGAGGGACAGGTGACAGAGCTGAGGAGCCGAGTGCAGGAGCTCGAGAGGGC TCTGGCAACTGCCAGGCAGGAGCACACGGAGCTGATGGAACAGTACAAGGGGATTTCCCGGTCCCATGGG GAGATCACAGAAGAGAGGGACATCCTGAGCCGGCAACAGGGAGACCATGTGGCACGCATCCTGGAGCTAG AGGATGACATCCAGACCATCAGTGAGAAAGTGCTGACGAAGGAAGTGGAGCTGGACAGGCTTAGAGACAC AGTGAAGGCCCTGACTCGGGAACAAGAGAAGCTCCTTGGGCAACTGAAAGAAGTACAAGCAGACAAGGAG CAAAGTGAGGCTGAGCTCCAAGTGGCACAACAGGAGAACCATCACTTAAATTTGGACCTGAAGGAGGCGA AGAGCTGGCAAGAGGAGCAGAGTGCTCAGGCTCAGCGACTGAAAGACAAGGTGGCCCAGATGAAGGACAC CCTAGGCCAGGCCCAGCAGCGGGTGGCCGAGCTGGAGCCCTTGAAGGAGCAGCTTCGAGGGGCCCAGGAG CTTGCAGCCTCAAGCCAGCAGAAAGCCACCCTTCTTGGGGAGGAGTTGGCCAGTGCAGCAGCAGCCAGGG ACCGCACCATAGCCGAACTACACCGCAGCCGCCTGGAAGTGGCTGAAGTTAACGGCAGGCTGGCTGAGCT CGGTTTGCACTTGAAGGAAGAAAAATGCCAATGGAGCAAGGAGCGGGCAGGGCTGCTGCAGAGTGTGGAG GCAGAGAAGGACAAGATCCTGAAGCTGAGTGCAGAGATACTTCGATTGGAGAAGGCAGTTCAGGAGGAGA GGACCCAAAACCAAGTGTTCAAGACTGAGCTGGCCCGGGAGAAGGATTCTAGCCTGGTACAGTTGTCAGA AAGTAAGCGGGAGCTGACAGAGCTGCGGTCAGCCCTGCGTGTGCTCCAGAAGGAAAAGGAGCAGTTACAG GAGGAGAAACAGGAATTGCTAGAGTACATGAGAAAGCTAGAGGCCCGCCTGGAGAAGGTGGCAGATGAGA AGTGGAATGAGGATGCCACCACAGAGGATGAGGAGGCCGCTGTGGGGCTGAGCTGCCCGGCAGCTCTGAC AGACTCAGAGGACGAGTCCCCAGAAGACATGAGGCTCCCACCCTATGGCCTTTGTGAGCGTGGAGACCCA GGCTCCTCTCCTGCTGGGCCTCGAGAGGCTTCTCCCCTTGTTGTCATCAGCCAGCCGGCTCCCATTTCTC CTCACCTCTCTGGGCCAGCTGAGGACAGTAGCTCTGACTCGGAGGCTGAAGATGAGAAGTCAGTCCTGAT GGCAGCTGTGCAGAGTGGGGGTGAGGAGGCCAACTTACTGCTTCCTGAACTGGGCAGTGCCTTCTATGAC ATGGCCAGTGGCTTTACAGTGGGTACCCTGTCAGAAACCAGCACTGGGGGCCCTGCCACCCCCACATGGA AGGAGTGTCCTATCTGTAAGGAGCGCTTTCCTGCTGAGAGTGACAAGGATGCCCTGGAGGACCACATGGA TGGACACTTCTTTTTCAGCACCCAGGACCCCTTCACCTTTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201375 protein sequence
Red=Cloning site Green=Tags(s) MEESPLSRAPSRGGVNFLNVARTYIPNTKVECHYTLPPGTMPSASDWIGIFKVEAACVRDYHTFVWSSVP ESTTDGSPIHTSVQFQASYLPKPGAQLYQFRYVNRQGQVCGQSPPFQFREPRPMDELVTLEEADGGSDIL LVVPKATVLQNQLDESQQERNDLMQLKLQLEGQVTELRSRVQELERALATARQEHTELMEQYKGISRSHG EITEERDILSRQQGDHVARILELEDDIQTISEKVLTKEVELDRLRDTVKALTREQEKLLGQLKEVQADKE QSEAELQVAQQENHHLNLDLKEAKSWQEEQSAQAQRLKDKVAQMKDTLGQAQQRVAELEPLKEQLRGAQE LAASSQQKATLLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAGLLQSVE AEKDKILKLSAEILRLEKAVQEERTQNQVFKTELAREKDSSLVQLSESKRELTELRSALRVLQKEKEQLQ EEKQELLEYMRKLEARLEKVADEKWNEDATTEDEEAAVGLSCPAALTDSEDESPEDMRLPPYGLCERGDP GSSPAGPREASPLVVISQPAPISPHLSGPAEDSSSDSEAEDEKSVLMAAVQSGGEEANLLLPELGSAFYD MASGFTVGTLSETSTGGPATPTWKECPICKERFPAESDKDALEDHMDGHFFFSTQDPFTFE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020898 |
| ORF Size | 2073 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020898.3 |
| RefSeq Size | 3046 bp |
| RefSeq ORF | 2076 bp |
| Locus ID | 57658 |
| UniProt ID | Q9P1Z2 |
| Protein Families | Transcription Factors |
| MW | 77.3 kDa |
| Gene Summary | Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions (By similarity). In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy
,Stefely, JA;Zhang, Y;Freiberger, EC;Kwiecien, NW;Thomas, HE;Davis, AM;Lowry, ND;Vincent, CE;Shishkova, E;Clark, NA;Medvedovic, M;Coon, JJ;Pagliarini, DJ;Mercer, CA;,
Autophagy
,PubMed ID 31971854
[CoCoA]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201375L3 | Lenti ORF clone of Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201375L4 | Lenti ORF clone of Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG201375 | CALCOCO1 (tGFP-tagged) - Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1 |
CNY 6,370.00 |
|
| SC108014 | CALCOCO1 (untagged)-Human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1 |
CNY 2,950.00 |
