ARIH2 (NM_006321) Human Tagged ORF Clone

CAT#: RC201846

ARIH2 (Myc-DDK-tagged)-Human ariadne homolog 2 (Drosophila) (ARIH2)

ORF Plasmid: DDK tGFP



  "NM_006321" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


ARIH2 mouse monoclonal antibody,clone OTI1B2
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ARI2; TRIAD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201846 representing NM_006321
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGAGGACTATGACCCAAATTGTGAGGAAG
AGGAAGAAGAAGAAGAAGACGACCCTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGA
GCAGCAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGACCTACAAGGAATCTGAG
GGTGCCCTCAATGAGCACATGACCAGCTTAGCTTCTGTCCTAAAGGTATCTCATTCAGTTGCTAAACTTA
TATTAGTTAATTTCCACTGGCAAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCAACTGCT
TGTTGAGGCTCGAGTTCAGCCTAATCCATCAAAACATGTTCCCACATCCCATCCCCCTCACCACTGTGCA
GTGTGTATGCAGTTTGTGCGAAAGGAAAACCTACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCT
GCTGGGAGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCTCTTGCATGGCTCAGGA
CTGTCCACTCCGTACACCAGAGGACTTTGTGTTTCCATTGCTTCCCAATGAAGAATTGAGAGAGAAATAC
AGGCGCTACCTCTTCAGGGACTATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTGCAGACTGCC
CCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTACAGTGCAATCGGTGCAACGAGGTCTTCTG
TTTCAAGTGTCGTCAGATGTATCACGCACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGT
GCAGACGACTCTGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCCAAGTGCAACATCTGCA
TTGAGAAGAATGGAGGCTGCAATCACATGCAATGCTCCAAATGTAAACACGACTTCTGCTGGATGTGTCT
AGGAGATTGGAAGACTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGAATCCTGACATCGTG
AACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTACTTATTCTACTTTGAGAGGTGGGAAA
ACCACAATAAAAGCTTGCAGCTAGAGGCACAGACATACCAGCGGATTCACGAGAAGATTCAGGAGAGGGT
CATGAACAATCTGGGGACATGGATCGACTGGCAGTACCTACAGAATGCTGCCAAGCTCTTGGCCAAGTGT
CGATACACCCTGCAATACACCTACCCATATGCATATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTG
AATACCAGCAGGCTCAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGACAGCTA
TGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGCGGAGGAGAACCCTGCTGAAAGATTTC
CATGACACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201846 representing NM_006321
Red=Cloning site Green=Tags(s)

MSVDMNSQGSDSNEEDYDPNCEEEEEEEEDDPGDIEDYYVGVASDVEQQGADAFDPEEYQFTCLTYKESE
GALNEHMTSLASVLKVSHSVAKLILVNFHWQVSEILDRYKSNSAQLLVEARVQPNPSKHVPTSHPPHHCA
VCMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKY
RRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKC
ADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIV
NQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKC
RYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDF
HDT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006321
ORF Size 1479 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006321.4
RefSeq Size 3892 bp
RefSeq ORF 1482 bp
Locus ID 10425
UniProt ID O95376
Domains IBR
Protein Families Druggable Genome
MW 57.6 kDa
Gene Summary The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet. [provided by RefSeq, Nov 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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