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HMG20A (NM_018200) Human Tagged ORF Clone
CAT＃: RC201894
HMG20A (Myc-DDK-tagged)-Human high mobility group 20A (HMG20A)
ORF Plasmid: tGFP
"NM_018200" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HMGX1; HMGXB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201894 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGTA ATGATCTGGCTACCACTGGGTTAAATCACCCAGAGGTTCCATACAGTAGTGGCGCCACATCATCCACCAA CAATCCAGAATTTGTGGAGGATCTCTCTCAAGGTCAGTTGCTTCAGAGTGAGTCTTCAAATGCAGCAGAA GGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGTTGGTCCAAAGGAAGAAAGAGGA AGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCCCTTACAGGATATGTTCGGTTCATGAATGAGCG TCGAGAACAACTTCGAGCAAAGAGACCAGAAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAA TGGAGTAAACTGCCTCCTGAGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACA TGAAGGAACTGGAACAGTATCAGAAAACAGAGGCCTACAAGGTCTTCAGTAGGAAAACCCAGGACCGTCA GAAAGGCAAATCTCATAGGCAAGATGCAGCCCGGCAGGCCACTCATGATCATGAGAAAGAAACAGAGGTA AAGGAACGGTCTGTTTTTGACATCCCTATATTTACAGAGGAATTCTTGAACCATAGCAAAGCTCGGGAAG CAGAGCTCCGCCAGCTTCGCAAATCCAACATGGAGTTTGAGGAGAGGAATGCAGCCCTGCAAAAGCACGT GGAGAGCATGCGCACAGCAGTGGAGAAGCTGGAGGTGGATGTGATCCAGGAGCGGAGCCGCAACACAGTC TTACAGCAGCACCTGGAGACCCTGCGGCAGGTGCTGACCAGCAGCTTTGCCAGCATGCCCTTGCCTGGAA GTGGAGAGACACCTACAGTGGACACCATTGACTCATATATGAACAGACTGCACAGTATTATTTTAGCTAA TCCCCAAGACAATGAAAACTTCATAGCTACAGTTCGAGAAGTTGTGAACAGACTCGATCGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201894 protein sequence
Red=Cloning site Green=Tags(s) MENLMTSSTLPPLFADEDGSKESNDLATTGLNHPEVPYSSGATSSTNNPEFVEDLSQGQLLQSESSNAAE GNEQRHEDEQRSKRGGWSKGRKRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNE WSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEV KERSVFDIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEKLEVDVIQERSRNTV LQQHLETLRQVLTSSFASMPLPGSGETPTVDTIDSYMNRLHSIILANPQDNENFIATVREVVNRLDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018200 |
| ORF Size | 1041 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018200.3 |
| RefSeq Size | 3898 bp |
| RefSeq ORF | 1044 bp |
| Locus ID | 10363 |
| UniProt ID | Q9NP66 |
| Domains | HMG |
| Protein Families | Transcription Factors |
| MW | 40.1 kDa |
| Gene Summary | Plays a role in neuronal differentiation as chromatin-associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the histone methyltransferase KMT2A/MLL1 and consequent increased methylation of histone H3 lysine 4 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201894L3 | Lenti ORF clone of Human high mobility group 20A (HMG20A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201894L4 | Lenti ORF clone of Human high mobility group 20A (HMG20A), mGFP tagged |
CNY 5,890.00 |
|
| RG201894 | HMG20A (tGFP-tagged) - Human high mobility group 20A (HMG20A) |
CNY 4,370.00 |
|
| SC113638 | HMG20A (untagged)-Human high mobility group 20A (HMG20A) |
CNY 3,656.00 |
