ICA1 (NM_004968) Human Tagged ORF Clone
CAT#: RC202149
ICA1 (Myc-DDK-tagged)-Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2
ORF Plasmid: tGFP
"NM_004968" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ICA69; ICAp69 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202149 representing NM_004968
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGACACAAATGTTATCCCTGGGACTTACAGGATCGATATGCTCAAGATAAGTCAGTTGTAAATA AGATGCAACAGAAATATTGGGAGACGAAGCAGGCCTTTATTAAAGCCACAGGGAAGAAGGAAGATGAACA TGTTGTTGCCTCTGACGCGGACCTGGATGCCAAGCTAGAGCTGTTTCATTCAATTCAGAGAACCTGTCTG GACTTATCGAAAGCAATTGTACTCTATCAAAAGAGGATATGTTTCTTGTCTCAAGAAGAAAACGAACTGG GAAAATTTCTTCGATCCCAAGGTTTCCAAGATAAAACCAGAGCAGGAAAGATGATGCAAGCGACAGGAAA GGCCCTCTGCTTTTCTTCCCAGCAAAGGTTGGCCTTACGAAATCCTTTGTGTCGATTTCACCAAGAAGTG GAGACTTTTCGGCATCGGGCCATCTCAGATACTTGGCTGACGGTGAACCGCATGGAACAGTGCAGGACGG AATATAGAGGAGCACTATTATGGATGAAGGACGTGTCTCAGGAGCTTGATCCAGACCTCTACAAGCAAAT GGAGAAGTTCAGGAAGGTACAAACACAAGTGCGCCTTGCAAAAAAAAACTTTGACAAATTGAAGATGGAT GTTTGTCAAAAAGTGGATCTTCTTGGAGCGAGCAGATGCAATCTCTTGTCTCACATGCTAGCAACATACC AGACCACTCTGCTTCATTTTTGGGAGAAAACTTCTCACACTATGGCAGCCATCCATGAGAGTTTCAAAGG TTATCAACCATATGAATTTACTACTTTAAAGAGCTTACAAGACCCTATGAAAAAATTAGTTGAGAAAGAA GAGAAGAAGAAAATCAACCAGCAGGAAAGTACAGATGCAGCCGTGCAGGAGCCGAGCCAATTAATTTCAT TAGAGGAAGAAAACCAGCGCAAGGAATCCTCTAGTTTTAAGACTGAAGATGGAAAAAGTATTTTATCTGC CTTAGACAAAGGCTCTACACATACTGCATGCTCAGGACCCATAGATGAACTATTAGACATGAAATCTGAG GAAGGTGCTTGCCTGGGACCAGTGGCAGGGACCCCGGAACCTGAAGGTGCTGACAAAGATGACCTGCTGC TGTTGAGTGAGATCTTCAATGCTTCCTCCTTGGAAGAGGGCGAGTTCAGCAAAGAGTGGGCCGCTGTGTT TGGAGACGGCCAAGTGAAGGAGCCAGTGCCCACTATGGCCCTGGGAGAGCCAGACCCCAAGGCCCAGACA GGCTCAGGTTTCCTTCCTTCGCAGCTTTTAGACCAAAATATGAAAGACTTACAGGCCTCGCTACAAGAAC CTGCTAAGGCTGCCTCAGACCTGACTGCCTGGTTCAGCCTCTTCGCTGACCTCGACCCACTCTCAAATCC TGATGCTGTTGGGAAAACCGATAAAGAACACGAATTGCTCAATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202149 representing NM_004968
Red=Cloning site Green=Tags(s) MSGHKCYPWDLQDRYAQDKSVVNKMQQKYWETKQAFIKATGKKEDEHVVASDADLDAKLELFHSIQRTCL DLSKAIVLYQKRICFLSQEENELGKFLRSQGFQDKTRAGKMMQATGKALCFSSQQRLALRNPLCRFHQEV ETFRHRAISDTWLTVNRMEQCRTEYRGALLWMKDVSQELDPDLYKQMEKFRKVQTQVRLAKKNFDKLKMD VCQKVDLLGASRCNLLSHMLATYQTTLLHFWEKTSHTMAAIHESFKGYQPYEFTTLKSLQDPMKKLVEKE EKKKINQQESTDAAVQEPSQLISLEEENQRKESSSFKTEDGKSILSALDKGSTHTACSGPIDELLDMKSE EGACLGPVAGTPEPEGADKDDLLLLSEIFNASSLEEGEFSKEWAAVFGDGQVKEPVPTMALGEPDPKAQT GSGFLPSQLLDQNMKDLQASLQEPAKAASDLTAWFSLFADLDPLSNPDAVGKTDKEHELLNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004968 |
ORF Size | 1446 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004968.3, NP_004959.2 |
RefSeq Size | 2396 bp |
RefSeq ORF | 1452 bp |
Locus ID | 3382 |
UniProt ID | Q05084 |
Domains | ICA69 |
Protein Pathways | Type I diabetes mellitus |
MW | 54.5 kDa |
Gene Summary | This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202149L1 | Lenti ORF clone of Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC202149L2 | Lenti ORF clone of Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC202149L3 | Lenti ORF clone of Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202149L4 | Lenti ORF clone of Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG202149 | ICA1 (tGFP-tagged) - Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2 |
CNY 5,256.00 |
|
SC117057 | ICA1 (untagged)-Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2 |
CNY 2,400.00 |
|
SC317806 | ICA1 (untagged)-Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2 |
CNY 7,220.00 |