COL8A1 (NM_020351) Human Tagged ORF Clone
CAT#: RC202240
COL8A1 (Myc-DDK-tagged)-Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 2
ORF Plasmid: tGFP
"NM_020351" in other vectors (5)
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CNY 5,448.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C3orf7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202240 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGCTGCCTGGCCCTCTGCAGCTGCTGGGAGTGCTGCTTACCATTTCCCTGAGTTCCATCAGGC TCATTCAGGCTGGTGCCTACTATGGGATCAAGCCGCTGCCACCTCAAATTCCTCCTCAGATGCCACCACA AATTCCACAATACCAGCCCCTGGGTCAGCAAGTACCTCACATGCCTTTGGCCAAAGATGGCCTTGCCATG GGCAAGGAGATGCCCCACTTGCAGTATGGCAAAGAGTATCCACACCTACCCCAATATATGAAGGAAATTC AACCGGCGCCAAGAATGGGCAAGGAAGCCGTACCCAAGAAAGGCAAAGAAATACCATTAGCCAGTTTACG AGGGGAACAAGGTCCCCGTGGAGAGCCTGGCCCAAGAGGACCACCTGGGCCCCCTGGTTTGCCAGGTCAT GGGATACCTGGAATTAAAGGAAAACCAGGGCCACAGGGATATCCAGGAGTTGGAAAGCCAGGTATGCCTG GAATGCCAGGGAAGCCAGGAGCCATGGGCATGCCTGGGGCAAAAGGAGAAATTGGACAGAAAGGGGAAAT TGGGCCTATGGGGATCCCAGGACCACAAGGACCTCCAGGGCCTCATGGACTTCCTGGCATTGGGAAGCCA GGTGGGCCAGGGTTACCAGGGCAACCAGGACCAAAGGGTGATCGAGGACCCAAAGGACTACCAGGACCTC AAGGCCTTCGGGGTCCTAAAGGAGACAAGGGCTTCGGGATGCCAGGTGCGCCAGGTGTAAAGGGGCCTCC AGGGATGCACGGCCCTCCCGGCCCTGTTGGACTGCCAGGAGTGGGCAAACCAGGAGTGACAGGCTTCCCT GGGCCCCAGGGCCCCCTGGGAAAGCCAGGGGCTCCAGGAGAACCTGGGCCACAAGGCCCTATTGGGGTAC CGGGGGTTCAAGGACCTCCTGGGATACCCGGAATTGGAAAGCCAGGCCAGGATGGGATCCCAGGCCAGCC AGGATTTCCAGGTGGCAAAGGGGAGCAAGGACTGCCAGGGCTACCAGGACCCCCAGGCCTTCCAGGGATT GGGAAACCAGGCTTCCCAGGACCCAAAGGTGACCGGGGCATGGGAGGTGTTCCTGGGGCTCTTGGACCAA GAGGGGAGAAAGGACCAATAGGTGCCCCAGGAATAGGGGGTCCTCCAGGAGAGCCAGGCCTGCCTGGAAT CCCAGGTCCTATGGGCCCTCCAGGTGCTATTGGTTTTCCTGGACCCAAAGGAGAAGGTGGGATTGTAGGG CCACAGGGGCCACCAGGTCCCAAGGGTGAGCCAGGGCTTCAAGGCTTCCCAGGAAAGCCAGGTTTCCTTG GTGAAGTAGGGCCTCCTGGCATGAGGGGTTTGCCAGGTCCCATAGGGCCCAAGGGGGAAGCTGGGCAAAA AGGTGTACCAGGACTCCCTGGTGTTCCAGGGCTTCTCGGACCTAAGGGAGAGCCAGGAATCCCAGGGGAT CAGGGTTTACAGGGCCCCCCAGGTATCCCAGGGATTGGGGGCCCTAGTGGCCCCATTGGACCACCTGGGA TTCCAGGCCCCAAAGGGGAGCCGGGCCTCCCAGGGCCCCCTGGGTTCCCTGGTATAGGGAAACCCGGAGT GGCAGGACTTCATGGCCCCCCAGGGAAGCCTGGTGCCCTTGGTCCTCAAGGCCAGCCTGGCCTTCCAGGA CCCCCAGGCCCTCCAGGACCTCCAGGACCCCCAGCTGTGATGCCCCCTACACCACCACCCCAGGGAGAGT ATCTGCCAGATATGGGGCTGGGAATTGATGGCGTGAAACCCCCCCATGCCTACGGGGCTAAGAAAGGCAA GAATGGAGGGCCAGCCTATGAGATGCCTGCATTTACCGCCGAGCTAACCGCACCTTTCCCACCGGTGGGG GCCCCAGTGAAGTTTAACAAACTGCTGTATAACGGCAGACAGAACTACAACCCGCAGACAGGCATCTTCA CCTGTGAGGTCCCTGGTGTCTACTACTTTGCATACCACGTTCACTGCAAGGGGGGCAACGTGTGGGTTGC TCTATTCAAGAACAACGAGCCCGTGATGTACACGTACGACGAGTACAAAAAGGGCTTCCTGGACCAGGCA TCTGGGAGTGCAGTGCTGCTGCTCAGGCCCGGAGACCGGGTGTTCCTCCAGATGCCCTCAGAACAGGCTG CAGGACTGTATGCCGGGCAGTATGTCCACTCCTCCTTTTCAGGATATTTATTGTATCCCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202240 protein sequence
Red=Cloning site Green=Tags(s) MAVLPGPLQLLGVLLTISLSSIRLIQAGAYYGIKPLPPQIPPQMPPQIPQYQPLGQQVPHMPLAKDGLAM GKEMPHLQYGKEYPHLPQYMKEIQPAPRMGKEAVPKKGKEIPLASLRGEQGPRGEPGPRGPPGPPGLPGH GIPGIKGKPGPQGYPGVGKPGMPGMPGKPGAMGMPGAKGEIGQKGEIGPMGIPGPQGPPGPHGLPGIGKP GGPGLPGQPGPKGDRGPKGLPGPQGLRGPKGDKGFGMPGAPGVKGPPGMHGPPGPVGLPGVGKPGVTGFP GPQGPLGKPGAPGEPGPQGPIGVPGVQGPPGIPGIGKPGQDGIPGQPGFPGGKGEQGLPGLPGPPGLPGI GKPGFPGPKGDRGMGGVPGALGPRGEKGPIGAPGIGGPPGEPGLPGIPGPMGPPGAIGFPGPKGEGGIVG PQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEPGIPGD QGLQGPPGIPGIGGPSGPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGPQGQPGLPG PPGPPGPPGPPAVMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAFTAELTAPFPPVG APVKFNKLLYNGRQNYNPQTGIFTCEVPGVYYFAYHVHCKGGNVWVALFKNNEPVMYTYDEYKKGFLDQA SGSAVLLLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGYLLYPM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020351 |
ORF Size | 2232 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020351.4 |
RefSeq Size | 3029 bp |
RefSeq ORF | 2235 bp |
Locus ID | 1295 |
UniProt ID | P27658 |
Protein Families | Druggable Genome |
MW | 73.4 kDa |
Gene Summary | This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202240L3 | Lenti ORF clone of Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202240L4 | Lenti ORF clone of Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG202240 | COL8A1 (tGFP-tagged) - Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 2 |
CNY 7,048.00 |
|
SC109090 | COL8A1 (untagged)-Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 2 |
CNY 5,456.00 |
|
SC323709 | COL8A1 (untagged)-Human collagen, type VIII, alpha 1 (COL8A1), transcript variant 2 |
CNY 5,456.00 |