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Elongin A (TCEB3) (NM_003198) Human Tagged ORF Clone
CAT＃: RC202462
TCEB3 (Myc-DDK-tagged)-Human transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) (TCEB3)
ORF Plasmid: tGFP
"NM_003198" in other vectors (5)
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CNY 5,656.00
CNY 8,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SIII; SIII p110; TCEB3; TCEB3A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202462 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGTCGGCGCTCCAAGTTGTGGAGAAGCTGCAGGCGCGCCTGGCCGCGAACCCGGACCCTA AGAAGCTATTGAAATATTTGAAGAAACTCTCCACCCTGCCTATTACAGTAGACATTCTTGCGGAGACTGG GGTTGGGAAAACAGTAAATAGCTTGCGAAAACACGAGCATGTTGGAAGCTTTGCCAGGGACCTAGTGGCC CAGTGGAAGAAGCTGGTTCCTGTGGAACGAAATGCTGAGCCTGATGAACAGGACTTTGAGAAGAGCAATT CCCGAAAGCGCCCTCGGGATGCCCTGCAGAAGGAGGAGGAGATGGAGGGGGACTACCAAGAAACCTGGAA AGCCACGGGGAGCCGATCCTATAGCCCTGACCACAGGCAGAAGAAACATAGGAAACTCTCGGAGCTCGAG AGACCTCACAAAGTGTCTCACGGTCATGAGAGGAGAGATGAGAGAAAGAGGTGTCACAGAATGTCACCAA CTTACTCTTCAGACCCTGAGTCTTCTGATTATGGCCATGTTCAATCCCCTCCATCTTGTACCAGTCCTCA TCAGATGTACGTCGACCACTACAGATCCCTGGAGGAGGACCAGGAGCCCATTGTTTCACACCAGAAGCCT GGGAAAGGCCACAGCAATGCCTTTCAGGACAGACTCGGGGCCAGCCAAGAACGACACCTGGGTGAACCCC ATGGGAAAGGGGTTGTGAGTCAAAACAAGGAGCACAAATCTTCCCACAAGGACAAACGCCCCGTGGATGC CAAGAGTGATGAGAAGGCCTCTGTGGTGAGCAGAGAGAAATCACACAAGGCCCTCTCCAAAGAGGAGAAC CGAAGGCCACCCTCAGGGGACAATGCAAGGGAGAAACCGCCCTCTAGTGGCGTAAAGAAAGAGAAGGACA GAGAGGGCAGCAGCCTGAAGAAGAAGTGTTTGCCTCCCTCAGAGGCCGCTTCAGACAACCACCTGAAAAA GCCAAAGCACAGAGACCCAGAGAAAGCCAAATTGGACAAAAGCAAGCAAGGTCTGGACAGCTTTGACACA GGAAAAGGAGCAGGAGACCTGTTGCCCAAGGTAAAAGAGAAGGGTTCTAACAACCTAAAGACTCCAGAAG GGAAAGTCAAAACTAATTTGGATAGAAAGTCACTGGGCTCCCTCCCTAAAGTTGAGGAGACAGATATGGA GGATGAATTCGAGCAGCCAACCATGTCTTTTGAATCCTACCTCAGCTATGACCAGCCCCGGAAGAAAAAG AAAAAGATTGTGAAAACTTCAGCCACGGCACTTGGAGATAAAGGACTTAAAAAAAATGACTCTAAAAGCA CTGGTAAAAACTTGGACTCAGTTCAGAAATTACCCAAGGTGAACAAAACCAAGTCAGAGAAGCCGGCTGG AGCTGATTTAGCCAAGCTGAGAAAGGTGCCTGATGTGTTGCCAGTGTTGCCAGACCTCCCGTTACCCGCG ATACAGGCCAATTACCGTCCACTGCCTTCCCTCGAGCTGATATCCTCCTTCCAGCCAAAGCGAAAAGCGT TCTCTTCACCCCAGGAAGAAGAAGAAGCTGGATTTACTGGGCGCAGAATGAATTCCAAGATGCAGGTGTA TTCTGGTTCCAAGTGTGCCTATCTCCCTAAAATGATGACCTTGCACCAGCAATGCATCCGAGTACTTAAA AACAACATCGATTCAATCTTTGAAGTGGGAGGAGTCCCATACTCTGTTCTTGAACCCGTTTTGGAGAGGT GTACACCTGATCAGCTGTATCGCATAGAGGAATACAATCATGTATTAATTGAAGAAACAGATCAATTATG GAAAGTTCATTGTCACCGAGACTTTAAGGAAGAAAGACCCGAAGAGTATGAGTCGTGGCGAGAGATGTAC CTGCGGCTTCAGGACGCCCGAGAGCAGCGGCTACGAGTACTAACAAAGAATATCCAGTTCGCACATGCCA ATAAGCCCAAAGGCCGACAAGCAAAGATGGCCTTTGTCAACTCTGTGGCCAAGCCACCTCGTGACGTCCG GAGGAGGCAGGAAAAGTTTGGAACGGGAGGAGCAGCTGTCCCTGAGAAAATCAAGATCAAGCCAGCCCCG TACCCCATGGGAAGCAGCCATGCTTCCGCCAGTAGTATCAGCTTTAACCCCAGCCCTGAGGAGCCGGCCT ATGATGGCCCAAGCACCAGCAGTGCCCACTTGGCACCAGTGGTCAGCAGCACTGTTTCCTATGATCCTAG GAAACCCACTGTGAAGAAAATTGCCCCAATGATGGCCAAGACAATTAAAGCTTTCAAGAACAGATTCTCC CGACGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202462 protein sequence
Red=Cloning site Green=Tags(s) MAAESALQVVEKLQARLAANPDPKKLLKYLKKLSTLPITVDILAETGVGKTVNSLRKHEHVGSFARDLVA QWKKLVPVERNAEPDEQDFEKSNSRKRPRDALQKEEEMEGDYQETWKATGSRSYSPDHRQKKHRKLSELE RPHKVSHGHERRDERKRCHRMSPTYSSDPESSDYGHVQSPPSCTSPHQMYVDHYRSLEEDQEPIVSHQKP GKGHSNAFQDRLGASQERHLGEPHGKGVVSQNKEHKSSHKDKRPVDAKSDEKASVVSREKSHKALSKEEN RRPPSGDNAREKPPSSGVKKEKDREGSSLKKKCLPPSEAASDNHLKKPKHRDPEKAKLDKSKQGLDSFDT GKGAGDLLPKVKEKGSNNLKTPEGKVKTNLDRKSLGSLPKVEETDMEDEFEQPTMSFESYLSYDQPRKKK KKIVKTSATALGDKGLKKNDSKSTGKNLDSVQKLPKVNKTKSEKPAGADLAKLRKVPDVLPVLPDLPLPA IQANYRPLPSLELISSFQPKRKAFSSPQEEEEAGFTGRRMNSKMQVYSGSKCAYLPKMMTLHQQCIRVLK NNIDSIFEVGGVPYSVLEPVLERCTPDQLYRIEEYNHVLIEETDQLWKVHCHRDFKEERPEEYESWREMY LRLQDAREQRLRVLTKNIQFAHANKPKGRQAKMAFVNSVAKPPRDVRRRQEKFGTGGAAVPEKIKIKPAP YPMGSSHASASSISFNPSPEEPAYDGPSTSSAHLAPVVSSTVSYDPRKPTVKKIAPMMAKTIKAFKNRFS RR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003198 |
| ORF Size | 2316 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003198.1, NP_003189.1 |
| RefSeq Size | 4959 bp |
| RefSeq ORF | 2319 bp |
| Locus ID | 6924 |
| UniProt ID | Q14241 |
| Domains | TFS2N |
| Protein Families | Transcription Factors |
| MW | 87.2 kDa |
| Gene Summary | This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202462L3 | Lenti ORF clone of Human transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) (TCEB3), Myc-DDK-tagged |
CNY 10,360.00 |
|
| RC202462L4 | Lenti ORF clone of Human transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) (TCEB3), mGFP tagged |
CNY 10,360.00 |
|
| RG202462 | TCEB3 (tGFP-tagged) - Human transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) (TCEB3) |
CNY 9,310.00 |
|
| SC118126 | TCEB3 (untagged)-Human transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) (TCEB3) |
CNY 5,664.00 |
|
| SC328012 | TCEB3 (untagged)-Human transcription elongation factor B (SIII) polypeptide 3 (110kDa elongin A) (TCEB3) |
CNY 15,390.00 |
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