PLK2 (NM_006622) Human Tagged ORF Clone
CAT#: RC202499
PLK2 (Myc-DDK-tagged)-Human polo-like kinase 2 (PLK2)
ORF Plasmid: tGFP
"NM_006622" in other vectors (7)
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CNY 7,528.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | hPlk2; hSNK; SNK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202499 representing NM_006622
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTTTTGCGGACTATCACCTACCAGCCAGCCGCCAGCACCAAAATGTGCGAGCAGGCGCTGGGCA AGGGTTGCGGAGCAGACTCGAAGAAGAAGCGGCCGCCGCAGCCCCCCGAGGAATCGCAGCCACCTCAGTC CCAGGCGCAAGTGCCCCCGGCGGCCCCTCACCACCATCACCACCATTCGCACTCGGGGCCGGAGATCTCG CGGATTATCGTCGACCCCACGACTGGGAAGCGCTACTGCCGGGGCAAAGTGCTGGGAAAGGGTGGCTTTG CAAAATGTTACGAGATGACAGATTTGACAAATAACAAAGTCTACGCCGCAAAAATTATTCCTCACAGCAG AGTAGCTAAACCTCATCAAAGGGAAAAGATTGACAAAGAAATAGAGCTTCACAGAATTCTTCATCATAAG CATGTAGTGCAGTTTTACCACTACTTCGAGGACAAAGAAAACATTTACATTCTCTTGGAATACTGCAGTA GAAGGTCAATGGCTCATATTTTGAAAGCAAGAAAGGTGTTGACAGAGCCAGAAGTTCGATACTACCTCAG GCAGATTGTGTCTGGACTGAAATACCTTCATGAACAAGAAATCTTGCACAGAGATCTCAAACTAGGGAAC TTTTTTATTAATGAAGCCATGGAACTAAAAGTTGGGGACTTCGGTCTGGCAGCCAGGCTAGAACCCTTGG AACACAGAAGGAGAACGATATGTGGTACCCCAAATTATCTCTCTCCTGAAGTCCTCAACAAACAAGGACA TGGCTGTGAATCAGACATTTGGGCCCTGGGCTGTGTAATGTATACAATGTTACTAGGGAGGCCCCCATTT GAAACTACAAATCTCAAAGAAACTTATAGGTGCATAAGGGAAGCAAGGTATACAATGCCGTCCTCATTGC TGGCTCCTGCCAAGCACTTAATTGCTAGTATGTTGTCCAAAAACCCAGAGGATCGTCCCAGTTTGGATGA CATCATTCGACATGACTTTTTTTTGCAGGGCTTCACTCCGGACAGACTGTCTTCTAGCTGTTGTCATACA GTTCCAGATTTCCACTTATCAAGCCCAGCTAAGAATTTCTTTAAGAAAGCAGCTGCTGCTCTTTTTGGTG GCAAAAAAGACAAAGCAAGATATATTGACACACATAATAGAGTGTCTAAAGAAGATGAAGACATCTACAA GCTTAGGCATGATTTGAAAAAGACTTCAATAACTCAGCAACCCAGCAAACACAGGACAGATGAGGAGCTC CAGCCACCTACCACCACAGTTGCCAGGTCTGGAACACCCGCAGTAGAAAACAAGCAGCAGATTGGGGATG CTATTCGGATGATAGTCAGAGGGACTCTTGGCAGCTGTAGCAGCAGCAGTGAATGCCTTGAAGACAGTAC CATGGGAAGTGTTGCAGACACAGTGGCAAGGGTTCTTCGGGGATGTCTGGAAAACATGCCGGAAGCTGAT TGCATTCCCAAAGAGCAGCTGAGCACATCATTTCAGTGGGTCACCAAATGGGTTGATTACTCTAACAAAT ATGGCTTTGGGTACCAGCTCTCAGACCACACCGTCGGTGTCCTTTTCAACAATGGTGCTCACATGAGCCT CCTTCCAGACAAAAAAACAGTTCACTATTACGCAGAGCTTGGCCAATGCTCAGTTTTCCCAGCAACAGAT GCTCCTGAGCAATTTATTAGTCAAGTGACGGTGCTGAAATACTTTTCTCATTACATGGAGGAGAACCTCA TGGATGGTGGAGATCTGCCTAGTGTTACTGATATTCGAAGACCTCGGCTCTACCTCCTTCAGTGGCTAAA ATCTGATAAGGCCCTAATGATGCTCTTTAATGATGGCACCTTTCAGGTGAATTTCTACCATGATCATACA AAAATCATCATCTGTAGCCAAAATGAAGAATACCTTCTCACCTACATCAATGAGGATAGGATATCTACAA CTTTCAGGCTGACAACTCTGCTGATGTCTGGCTGTTCATCAGAATTAAAAAATCGAATGGAATATGCCCT GAACATGCTCTTACAAAGATGTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202499 representing NM_006622
Red=Cloning site Green=Tags(s) MELLRTITYQPAASTKMCEQALGKGCGADSKKKRPPQPPEESQPPQSQAQVPPAAPHHHHHHSHSGPEIS RIIVDPTTGKRYCRGKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRILHHK HVVQFYHYFEDKENIYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEILHRDLKLGN FFINEAMELKVGDFGLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCESDIWALGCVMYTMLLGRPPF ETTNLKETYRCIREARYTMPSSLLAPAKHLIASMLSKNPEDRPSLDDIIRHDFFLQGFTPDRLSSSCCHT VPDFHLSSPAKNFFKKAAAALFGGKKDKARYIDTHNRVSKEDEDIYKLRHDLKKTSITQQPSKHRTDEEL QPPTTTVARSGTPAVENKQQIGDAIRMIVRGTLGSCSSSSECLEDSTMGSVADTVARVLRGCLENMPEAD CIPKEQLSTSFQWVTKWVDYSNKYGFGYQLSDHTVGVLFNNGAHMSLLPDKKTVHYYAELGQCSVFPATD APEQFISQVTVLKYFSHYMEENLMDGGDLPSVTDIRRPRLYLLQWLKSDKALMMLFNDGTFQVNFYHDHT KIIICSQNEEYLLTYINEDRISTTFRLTTLLMSGCSSELKNRMEYALNMLLQRCN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006622 |
ORF Size | 2055 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006622.4 |
RefSeq Size | 2972 bp |
RefSeq ORF | 2058 bp |
Locus ID | 10769 |
UniProt ID | Q9NYY3 |
Domains | pkinase, POLO_box, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
MW | 78.1 kDa |
Gene Summary | The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Phosphorylation of Synucleins by Members of the Polo-like Kinase Family
,Martial K. Mbefo, Katerina E. Paleologou, Ahmed Boucharaba, Abid Oueslati, Heinrich Schell, Margot Fournier, Diana Olschewski, Guowei Yin, Markus Zweckstetter, Eliezer Masliah, Philipp J. Kahle, Harald Hirling, and Hilal A. Lashuel,
J. Biol. Chem., Jan 2010; 285: 2807 - 2822
[PLK2]
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Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202499L1 | Lenti ORF clone of Human polo-like kinase 2 (PLK2), Myc-DDK-tagged |
CNY 9,928.00 |
|
RC202499L2 | Lenti ORF clone of Human polo-like kinase 2 (PLK2), mGFP tagged |
CNY 7,700.00 |
|
RC202499L3 | Lenti ORF clone of Human polo-like kinase 2 (PLK2), Myc-DDK-tagged |
CNY 7,700.00 |
|
RC202499L4 | Lenti ORF clone of Human polo-like kinase 2 (PLK2), mGFP tagged |
CNY 9,928.00 |
|
RG202499 | PLK2 (tGFP-tagged) - Human polo-like kinase 2 (PLK2) |
CNY 9,128.00 |
|
SC115988 | PLK2 (untagged)-Human polo-like kinase 2 (PLK2) |
CNY 5,528.00 |
|
SC323362 | PLK2 (untagged)-Kinase deficient mutant (K111M) of Human polo-like kinase 2 (Drosophila) (PLK2) |
CNY 7,536.00 |