PLK2 (NM_006622) Human Untagged Clone

CAT#: SC323362

PLK2 (untagged)-Kinase deficient mutant (K111M) of Human polo-like kinase 2 (Drosophila) (PLK2)



  "NM_006622" in other vectors (7)

CNY 7,536.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
PLK2 Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms hPlk2; hSNK; SNK
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323362 sequence for NM_006622 edited (data generated by NextGen Sequencing)
ATGGAGCTTTTGCGGACTATCACCTACCAGCCAGCCGCCAGCACCAAAATGTGCGAGCAG
GCGCTGGGCAAGGGTTGCGGAGCGGACTCGAAGAAGAAGCGGCCGCCGCAGCCCCCCGAG
GAATCGCAGCCACCTCAGTCCCAGGCGCAAGTGCCCCCGGCGGCCCCTCACCACCATCAC
CACCATTCGCACTCGGGGCCGGAGATCTCGCGGATTATCGTCGACCCCACGACTGGGAAG
CGCTACTGCCGGGGCAAAGTGCTGGGAAAGGGTGGCTTTGCAAAATGTTACGAGATGACA
GATTTGACAAATAACAAAGTCTACGCCGCAATGATTATTCCTCACAGCAGAGTAGCTAAA
CCTCATCAAAGGGAAAAGATTGACAAAGAAATAGAGCTTCACAGAATTCTTCATCATAAG
CATGTAGTGCAGTTTTACCACTACTTCGAGGACAAAGAAAACATTTACATTCTCTTGGAA
TACTGCAGTAGAAGGTCAATGGCTCATATTTTGAAAGCAAGAAAGGTGTTGACAGAGCCA
GAAGTTCGATACTACCTCAGGCAGATTGTGTCTGGACTGAAATACCTTCATGAACAAGAA
ATCTTGCACAGAGATCTCAAACTAGGGAACTTTTTTATTAATGAAGCCATGGAACTAAAA
GTTGGGGACTTCGGTCTGGCAGCCAGGCTAGAACCCTTGGAACACAGAAGGAGAACGATA
TGTGGTACCCCAAATTATCTCTCTCCTGAAGTCCTCAACAAACAAGGACATGGCTGTGAA
TCAGACATTTGGGCCCTGGGCTGTGTAATGTATACAATGTTACTAGGGAGGCCCCCATTT
GAAACTACAAATCTCAAAGAAACTTACAGGTGCATAAGGGAAGCAAGGTATACAATGCCA
TCCTCATTGCTGGCTCCTGCCAAGCACTTAATTGCTAGTATGTTGTCCAAAAACCCAGAG
GATCGTCCCAGTTTGGATGACATCATTCGACATGACTTTTTTTTGCAGGGCTTCACTCCG
GACAGACTGTCTTCTAGCTGTTGTCATACAGTTCCAGATTTCCACTTATCAAGCCCAGCT
AAGAATTTCTTTAAGAAAGCAGCTGCTGCTCTTTTTGGTGGCAAAAAAGACAAAGCAAGA
TATATTGACACACATAATAGAGTGTCTAAAGAAGATGAAGACATCTACAAGCTTAGGCAT
GATTTGAAAAAGACTTCAATAACTCAGCAACCCAGCAAACACAGGACAGATGAGGAGCTC
CAGCCACCTACCACCACAGTTGCCAGGTCTGGAACACCCGCAGTAGAAAACAAGCAGCAG
ATTGGGGATGCTATTCGGATGATAGTCAGAGGGACTCTTGGCAGCTGTAGCAGCAGCAGT
GAATGCCTTGAAGACAGTACCATGGGAAGTGTTGCAGACACAGTGGCAAGGGTTCTTCGG
GGATGTCTGGAAAACATGCCGGAAGCTGATTGCATTCCCAAAGAGCAGCTGAGCACATCA
TTTCAGTGGGTCACCAAATGGGTTGATTACTCTAACAAATATGGCTTCGGGTACCAGCTC
TCAGACCACACCGTCGGTGTCCTTTTCAACAATGGTGCTCACATGAGCCTCCTTCCAGAC
AAAAAAACAGTTCACTATTACGCAGAGCTTGGCCAATGCTCAGTTTTCCCAGCAACAGAT
GCTCCTGAGCAATTTATTAGTCAAGTGACGGTGCTGAAATACTTTTCTCATTACATGGAG
GAGAACCTCATGGATGGTGGAGATCTGCCTAGTGTTACTGATATTCGAAGACCTCGGCTC
TACCTCCTTCAGTGGCTAAAATCTGATAAGGCCCTAATGATGCTCTTTAATGATGGCACC
TTTCAGGTGAATTTCTACCATGATCATACAAAAATCATCATCTGTAGCCAAAATGAAGAA
TACCTTCTCACCTACATCAATGAGGATAGGATATCTACAACTTTCAGGCTGACAACTCTG
CTGATGTCTGGCTGTTCATCAGAATTAAAAAATCGAATGGAATATGCCCTGAACATGCTC
TTACAAAGATGTAACTGA

Clone variation with respect to NM_006622.2
332 a=>t;333 a=>g;867 t=>c;900 g=>a;1548 t=>c
>OriGene 5' read for mutant NM_006622 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGGTGTTGGGT
GCTAGTCGGCACCAGAGGCAAGGGTGCGAGGACCACGGCCGGCTCGGACGTGTGACCGCGCCTAGGGGGT
GGCAGCGGGCAGTGCGGGGCGGCAAGGCGACCATGGAGCTTTTGCGGACTATCACCTACCAGCCAGCCGC
CAGCACCAAAATGTGCGAGCAGGCGCTGGGCAAGGGTTGCGGAGCGGACTCGAAGAAGAAGCGGGTCGCT
GTCGGCCCCCCGAGGATTCGCAGCCCACCTCAGTCCCAGGCGCAGGTGCCCCCGGCGGCCCCTCACCACC
ATCACCACCATTCGCACTCGGGGCCGGAAATCTCGCGGATTATCGTCGACCCACGACTGGGAGCCGCTAC
TGCCGGGGCAAAGTGCTGGGAAGGGTTGGCTTTGCAAATGGTTACGAAAAGCAGGATTGCCAATACAAAG
CCAGCCCCATTAATATTCCTAACGAGATAGTTAACCCTATCAAGGGAAAGATGCAAGAATAGCCTCACAG
AATCTCTCACAGCAGTGAGCGAGTTTCCCTACTCAGAGACAAGAACATTACTCCTGGATCGCGATAAGTC
AGTGCTAATTGACGAAGGTTCAGACCAATCTATCCTCAGCATTGTGGCAGACCTGACGAACTGCGATTCC
ATGGACTTCTATGACGGCATGGACCCCCGCGCGCACTGACACGACAAGTGGCCTGC
>SC323362 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GKWSSRRRSMWTCGTGCGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGT
TTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGG
TGTTGGGTGCTAGTCGGCACCAGAGGCAAGGGTGCGAGGACCACGGCCGGCTCGGACGTGTGACCGCGCC
TAGGGGGTGGCAGCGGGCAGTGCGGGGCGGCAAGGCGACCATGGA
Restriction Sites Please inquire     
ACCN NM_006622
Insert Size 2690 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006622.1, NP_006613.1
RefSeq Size 2972 bp
RefSeq ORF 2058 bp
Locus ID 10769
UniProt ID Q9NYY3
Domains pkinase, POLO_box, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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