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Glycophorin A (GYPA) (NM_002099) Human Tagged ORF Clone
CAT＃: RC202751
- TrueORF®
GYPA (Myc-DDK-tagged)-Human glycophorin A (MNS blood group) (GYPA)
ORF Plasmid: tGFP
"NM_002099" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD235a; GPA; GPErik; GPSAT; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202751 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGGAAAAATAATCTTTGTATTACTATTGTCAGCAATTGTGAGCATATCAGCATTAAGTACCACTG AGGTGGCAATGCACACTTCAACCTCTTCTTCAGTCACAAAGAGTTACATCTCATCACAGACAAATGATAC GCACAAACGGGACACATATGCAGCCACTCCTAGAGCTCATGAAGTTTCAGAAATTTCTGTTAGAACTGTT TACCCTCCAGAAGAGGAAACCGGAGAAAGGGTACAACTTGCCCATCATTTCTCTGAACCAGAGATAACAC TCATTATTTTTGGGGTGATGGCTGGTGTTATTGGAACGATCCTCTTAATTTCTTACGGTATTCGCCGACT GATAAAGAAAAGCCCATCTGATGTAAAACCTCTCCCCTCACCTGACACAGACGTGCCTTTAAGTTCTGTT GAAATAGAAAATCCAGAGACAAGTGATCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202751 protein sequence
Red=Cloning site Green=Tags(s) MYGKIIFVLLLSAIVSISALSTTEVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTV YPPEEETGERVQLAHHFSEPEITLIIFGVMAGVIGTILLISYGIRRLIKKSPSDVKPLPSPDTDVPLSSV EIENPETSDQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002099 |
| ORF Size | 450 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002099.8 |
| RefSeq Size | 2660 bp |
| RefSeq ORF | 453 bp |
| Locus ID | 2993 |
| UniProt ID | P02724 |
| Domains | Glycophorin_A |
| Protein Families | ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Hematopoietic cell lineage |
| MW | 16.4 kDa |
| Gene Summary | Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202751L1 | Lenti ORF clone of Human glycophorin A (MNS blood group) (GYPA), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC202751L2 | Lenti ORF clone of Human glycophorin A (MNS blood group) (GYPA), mGFP tagged |
CNY 5,890.00 |
|
| RC202751L3 | Lenti ORF clone of Human glycophorin A (MNS blood group) (GYPA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202751L4 | Lenti ORF clone of Human glycophorin A (MNS blood group) (GYPA), mGFP tagged |
CNY 3,600.00 |
|
| RG202751 | GYPA (tGFP-tagged) - Human glycophorin A (MNS blood group) (GYPA) |
CNY 2,800.00 |
|
| SC319730 | GYPA (untagged)-Human glycophorin A (MNS blood group) (GYPA) |
CNY 1,200.00 |
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