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HIF1AN (NM_017902) Human Tagged ORF Clone
CAT＃: RC202843
HIF1AN (Myc-DDK-tagged)-Human hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN)
ORF Plasmid: tGFP
"NM_017902" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FIH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202843 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGACAGCGGCGGAGGCTGTGGCCTCTGGCTCTGGAGAGCCCCGGGAGGAGGCTGGAGCCCTCG GCCCCGCCTGGGATGAATCCCAGTTGCGCAGTTATAGCTTCCCGACTAGGCCCATTCCGCGTCTGAGTCA GAGCGACCCCCGGGCAGAGGAGCTTATTGAGAATGAGGAGCCTGTGGTGCTGACCGACACAAATCTTGTG TATCCTGCCCTGAAATGGGACCTTGAATACCTGCAAGAGAATATTGGCAATGGAGACTTCTCTGTGTACA GTGCCAGCACCCACAAGTTCTTGTACTATGATGAGAAGAAGATGGCCAATTTCCAGAACTTTAAGCCGAG GTCCAACAGGGAAGAAATGAAATTTCATGAGTTCGTTGAGAAACTGCAGGATATACAGCAGCGAGGAGGG GAAGAGAGGTTGTATCTGCAGCAAACGCTCAATGACACTGTGGGCAGGAAGATTGTCATGGACTTCTTAG GTTTTAACTGGAACTGGATTAATAAGCAACAGGGAAAGCGTGGCTGGGGGCAGCTTACCTCTAACCTGCT GCTCATTGGCATGGAAGGAAATGTGACACCTGCTCACTATGATGAGCAGCAGAACTTTTTTGCTCAGATA AAAGGTTACAAACGATGCATCTTATTCCCTCCGGATCAGTTCGAGTGCCTCTACCCATACCCTGTTCATC ACCCATGTGACAGACAGAGCCAGGTGGACTTTGACAATCCCGACTACGAGAGGTTCCCTAATTTCCAAAA TGTGGTTGGTTACGAAACAGTGGTTGGCCCTGGTGATGTTCTTTACATCCCAATGTACTGGTGGCATCAC ATAGAGTCATTACTAAATGGGGGGATTACCATCACTGTGAACTTCTGGTATAAGGGGGCTCCCACCCCTA AGAGAATTGAATATCCTCTCAAAGCTCATCAGAAAGTGGCCATAATGAGAAACATTGAGAAGATGCTTGG AGAGGCCTTGGGGAACCCACAAGAGGTGGGGCCCTTGTTGAACACAATGATCAAGGGCCGATACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202843 protein sequence
Red=Cloning site Green=Tags(s) MAATAAEAVASGSGEPREEAGALGPAWDESQLRSYSFPTRPIPRLSQSDPRAEELIENEEPVVLTDTNLV YPALKWDLEYLQENIGNGDFSVYSASTHKFLYYDEKKMANFQNFKPRSNREEMKFHEFVEKLQDIQQRGG EERLYLQQTLNDTVGRKIVMDFLGFNWNWINKQQGKRGWGQLTSNLLLIGMEGNVTPAHYDEQQNFFAQI KGYKRCILFPPDQFECLYPYPVHHPCDRQSQVDFDNPDYERFPNFQNVVGYETVVGPGDVLYIPMYWWHH IESLLNGGITITVNFWYKGAPTPKRIEYPLKAHQKVAIMRNIEKMLGEALGNPQEVGPLLNTMIKGRYN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017902 |
| ORF Size | 1047 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017902.3 |
| RefSeq Size | 6928 bp |
| RefSeq ORF | 1050 bp |
| Locus ID | 55662 |
| UniProt ID | Q9NWT6 |
| Protein Families | Druggable Genome |
| MW | 40.3 kDa |
| Gene Summary | Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MiR-24 induces chemotherapy resistance and hypoxic advantage in breast cancer
,Roscigno, G;Puoti, I;Giordano, I;Donnarumma, E;Russo, V;Affinito, A;Adamo, A;Quintavalle, C;Todaro, M;Vivanco, MD;Condorelli, G;,
Oncotarget
,PubMed ID 28061479
[HIF1AN]
|
|
Snail is a Direct Target of HIF1a in Hypoxia-induced Endothelial to Mesenchymal Transition of Human Coronary Endothelial Cells
,Xu, X;Tan, X;Tampe, B;Sanchez, E;Zeisberg, M;Zeisberg, EM;,
J. Biol. Chem.
,PubMed ID 25971970
[HIF1AN]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202843L1 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202843L2 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN), mGFP tagged |
CNY 6,056.00 |
|
| RC202843L3 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202843L4 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN), mGFP tagged |
CNY 5,890.00 |
|
| RG202843 | HIF1AN (tGFP-tagged) - Human hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN) |
CNY 5,256.00 |
|
| SC319170 | HIF1AN (untagged)-Human hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN) |
CNY 3,656.00 |
