alpha Actinin 4 (ACTN4) (NM_004924) Human Tagged Lenti ORF Clone
CAT#: RC202873L2
- LentiORF®
Lenti ORF clone of Human actinin, alpha 4 (ACTN4), mGFP tagged
"NM_004924" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 12,408.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | ACTININ-4; FSGS; FSGS1 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC202873).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_004924 |
ORF Size | 2733 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004924.3 |
RefSeq Size | 3966 bp |
RefSeq ORF | 2736 bp |
Locus ID | 81 |
UniProt ID | O43707 |
Domains | CH, spectrin, EFh |
Protein Families | Druggable Genome |
Protein Pathways | Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction |
MW | 104.9 kDa |
Gene Summary | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202873 | ACTN4 (Myc-DDK-tagged)-Human actinin, alpha 4 (ACTN4) |
CNY 10,008.00 |
|
RC202873L1 | Lenti ORF clone of Human actinin, alpha 4 (ACTN4), Myc-DDK-tagged |
CNY 12,408.00 |
|
RC202873L3 | Lenti ORF clone of Human actinin, alpha 4 (ACTN4), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202873L4 | Lenti ORF clone of Human actinin, alpha 4 (ACTN4), mGFP tagged |
CNY 12,408.00 |
|
RG202873 | ACTN4 (tGFP-tagged) - Human actinin, alpha 4 (ACTN4) |
CNY 11,608.00 |
|
SC319140 | ACTN4 (untagged)-Human actinin, alpha 4 (ACTN4) |
CNY 10,016.00 |
其它alpha Actinin 4产品