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Twist (TWIST1) (NM_000474) Human Tagged ORF Clone
CAT＃: RC202920
TWIST1 (Myc-DDK-tagged)-Human twist homolog 1 (Drosophila) (TWIST1)
ORF Plasmid: tGFP
"NM_000474" in other vectors (6)
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CNY 3,600.00
CNY 3,705.00
| Cited in 8 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACS3; bHLHa38; BPES2; BPES3; CRS; CRS1; CSO; SCS; SWCOS; TWIST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202920 representing NM_000474.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGCAGGACGTGTCCAGCTCGCCAGTCTCGCCGGCCGACGACAGCCTGAGCAACAGCGAGGAAGAG CCAGACCGGCAGCAGCCGCCGAGCGGCAAGCGCGGGGGACGCAAGCGGCGCAGCAGCAGGCGCAGCGCG GGCGGCGGCGCGGGGCCCGGCGGAGCCGCGGGTGGGGGCGTCGGAGGCGGCGACGAGCCGGGCAGCCCG GCCCAGGGCAAGCGCGGCAAGAAGTCTGCGGGCTGTGGCGGCGGCGGCGGCGCGGGCGGCGGCGGCGGC AGCAGCAGCGGCGGCGGGAGTCCGCAGTCTTACGAGGAGCTGCAGACGCAGCGGGTCATGGCCAACGTG CGGGAGCGCCAGCGCACCCAGTCGCTGAACGAGGCGTTCGCCGCGCTGCGGAAGATCATCCCCACGCTG CCCTCGGACAAGCTGAGCAAGATTCAGACCCTCAAGCTGGCGGCCAGGTACATCGACTTCCTCTACCAG GTCCTCCAGAGCGACGAGCTGGACTCCAAGATGGCAAGCTGCAGCTATGTGGCTCACGAGCGGCTCAGC TACGCCTTCTCGGTCTGGAGGATGGAGGGGGCCTGGTCCATGTCCGCGTCCCAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA >Peptide sequence encoded by RC202920
Blue=ORF Red=Cloning site Green=Tag(s) MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSP AQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTL PSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC202920 also available, TP302920 |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000474 |
| ORF Size | 606 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000474.4 |
| RefSeq Size | 1669 bp |
| RefSeq ORF | 609 bp |
| Locus ID | 7291 |
| UniProt ID | Q15672 |
| Protein Families | Druggable Genome |
| MW | 21 kDa |
| Gene Summary | This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Jul 2020] |
Citations (8)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Overexpression of the nucleoporin Nup88 stimulates migration and invasion of HeLa cells
,null,
Histochemistry and Cell Biology
,PubMed ID 34331103
[Twist]
|
|
PLCε regulates prostate cancer mitochondrial oxidative metabolism and migration via upregulation of Twist1
,Fan, J;Fan, Y;Wang, X;Niu, L;Duan, L;Yang, J;Li, L;Gao, Y;Wu, X;Luo, C;,
J. Exp. Clin. Cancer Res.
,PubMed ID 31383001
[Twist]
|
|
α-Parvin promotes breast cancer progression and metastasis through interaction with G3BP2 and regulation of TWIST1 signaling
,Sun, Y;Ding, Y;Guo, C;Liu, C;Ma, P;Ma, S;Wang, Z;Liu, J;Qian, T;Ma, L;Deng, Y;Wu, C;,
Oncogene
,PubMed ID 30804457
[Twist]
|
|
The FZD7-TWIST1 axis is responsible for anoikis resistance and tumorigenesis in ovarian carcinoma
,Tan, M;Asad, M;Heong, V;Wong, MK;Tan, TZ;Ye, J;Kuay, KT;Thiery, JP;Scott, C;Huang, RY;,
Mol Oncol
,PubMed ID 30548372
[Twist]
|
|
TWIST1 induces MMP3 expression through up-regulating DNA hydroxymethylation and promotes catabolic responses in human chondrocytes
,Hasei, J;Teramura, T;Takehara, T;Onodera, Y;Horii, T;Olmer, M;Hatada, I;Fukuda, K;Ozaki, T;Lotz, MK;Asahara, H;,
Sci Rep
,PubMed ID 28220902
[Twist]
|
|
Transcription factors related to chondrogenesis in pleomorphic adenoma of the salivary gland: a mechanism of mesenchymal tissue formation.
,null,
Laboratory investigation; a journal of technical methods and pathology
,PubMed ID 26501866
[Twist]
|
|
The Role of TWIST in Angiogenesis and Cell Migration in Giant Cell Tumor of Bone
,Singh, S;Mak, IWY;Handa, D;Ghert, M;,
Advances in Biology
[Twist]
|
|
Interaction between docetaxel resistance and castration resistance in prostate cancer: Implications of twist1, YB-1, and androgen receptor
,null,
The Prostate
,PubMed ID 23775496
[Twist]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202920L1 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC202920L2 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged |
CNY 6,000.00 |
|
| RC202920L3 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC202920L4 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged |
CNY 6,000.00 |
|
| RG202920 | TWIST1 (tGFP-tagged) - Human twist homolog 1 (Drosophila) (TWIST1) |
CNY 5,200.00 |
|
| SC321467 | TWIST1 (untagged)-Human twist homolog 1 (Drosophila) (TWIST1) |
CNY 2,400.00 |
