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Twist (TWIST1) (NM_000474) Human Untagged Clone
CAT＃: SC321467
TWIST1 (untagged)-Human twist homolog 1 (Drosophila) (TWIST1)
CNY 2,400.00
CNY 3,990.00
| Cited in 7 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACS3; bHLHa38; BPES2; BPES3; CRS; CRS1; CSO; SCS; SWCOS; TWIST |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_000474.3
GAGGTATAAGAGCCTCCAAGTCTGCAGCTCTCGCCCAACTCCCAGACACCTCGCGGGCTC
TGCAGCACCGGCACCGTTTCCAGGAGGCCTGGCGGGGTGTGCGTCCAGCCGTTGGGCGCT TTCTTTTTGGACCTCGGGGCCATCCACACCGTCCCCTCCCCCTCCCGCCTCCCTCCCCGC CTCCCCCGCGCGCCCTCCCCGCGGAGGTCCCTCCCGTCCGTCCTCCTGCTCTCTCCTCCG CGGGCCGCATCGCCCGGGCCGGCGCCGCGCGCGGGGGAAGCTGGCGGGCTGAGGCGCCCC GCTCTTCTCCTCTGCCCCGGGCCCGCGAGGCCACGCGTCGCCGCTCGAGAGATGATGCAG GACGTGTCCAGCTCGCCAGTCTCGCCGGCCGACGACAGCCTGAGCAACAGCGAGGAAGAG CCAGACCGGCAGCAGCCGCCGAGCGGCAAGCGCGGGGGACGCAAGCGGCGCAGCAGCAGG CGCAGCGCGGGCGGCGGCGCGGGGCCCGGCGGAGCCGCGGGTGGGGGCGTCGGAGGCGGC GACGAGCCGGGCAGCCCGGCCCAGGGCAAGCGCGGCAAGAAGTCTGCGGGCTGTGGCGGC GGCGGCGGCGCGGGCGGCGGCGGCGGCAGCAGCAGCGGCGGCGGGAGTCCGCAGTCTTAC GAGGAGCTGCAGACGCAGCGGGTCATGGCCAACGTGCGGGAGCGCCAGCGCACCCAGTCG CTGAACGAGGCGTTCGCCGCGCTGCGGAAGATCATCCCCACGCTGCCCTCGGACAAGCTG AGCAAGATTCAGACCCTCAAGCTGGCGGCCAGGTACATCGACTTCCTCTACCAGGTCCTC CAGAGCGACGAGCTGGACTCCAAGATGGCAAGCTGCAGCTATGTGGCTCACGAGCGGCTC AGCTACGCCTTCTCGGTCTGGAGGATGGAGGGGGCCTGGTCCATGTCCGCGTCCCACTAG CAGGCGGAGCCCCCCACCCCCTCAGCAGGGCCGGAGACCTAGATGTCATTGTTTCCAGAG AAGGAGAAAATGGACAGTCTAGAGACTCTGGAGCTGGATAACTAAAAATAAAAATATATG CCAAAGATTTTCTTGGAAATTAGAAGAGCAAAATCCAAATTCAAAGAAACAGGGCGTGGG GCGCACTTTTAAAAGAGAAAGCGAGACAGGCCCGTGGACAGTGATTCCCAGACGGGCAGC GGCACCATCCTCACACCTCTGCATTCTGATAGAAGTCTGAACAGTTGTTTGTGTTTTTTT TTTTTTTTTTTGACGAAGAATGTTTTTATTTTTATTTTTTTCATGCATGCATTCTCAAGA GGTCGTGCCAATCAGCCACTGAAAGGAAAGGCATCACTATGGACTTTCTCTATTTTAAAA TGGTAACAATCAGAGGAACTATAAGAACACCTTTAGAAATAAAAATACTGGGATCAAACT GGCCTGCAAAACCATAGTCAGTTAATTCTTTTTTTCATCCTTCCTCTGAGGGGAAAAACA AAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000474 |
| Insert Size | 609 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000474.3, NP_000465.1 |
| RefSeq Size | 1669 bp |
| RefSeq ORF | 609 bp |
| Locus ID | 7291 |
| UniProt ID | Q15672 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Jul 2020] |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Knockdown of PLOD3 suppresses the malignant progression of renal cell carcinoma via reducing TWIST1 expression
,Xie, D;Li, J;Wei, S;Qi, P;Ji, H;Su, J;Du, N;Zhang, X;,
Mol. Cell. Probes
,PubMed ID 32585183
[TWIST1]
|
|
Rhodopsin T17M Mutant Inhibits Complement C3 Secretion in Retinal Pigment Epithelium via ROS Induced Downregulation of TWIST1.
,null,
Journal of cellular biochemistry
,PubMed ID 28569420
[TWIST1]
|
|
Activation of TWIST1 by COL11A1 promotes chemoresistance and inhibits apoptosis in ovarian cancer cells by modulating NF-κB-mediated IKKβ expression.
,null,
International journal of cancer
,PubMed ID 28815582
[TWIST1]
|
|
EZH2 inhibition suppresses endometrial cancer progression via miR-361/Twist axis
,Ihira, K;Dong, P;Xiong, Y;Watari, H;Konno, Y;Hanley, SJ;Noguchi, M;Hirata, N;Suizu, F;Yamada, T;Kudo, M;Sakuragi, N;,
Oncotarget
,PubMed ID 28088786
[TWIST1]
|
|
Twist1 Is a TNF-Inducible Inhibitor of Clock Mediated Activation of Period Genes
,Meier, D;Lopez, M;Franken, P;Fontana, A;,
PLoS ONE
,PubMed ID 26361389
[TWIST1]
|
|
Silencing of CDC42 inhibits neuroblastoma cell proliferation and transformation
,Lee, S;Craig, BT;Romain, CV;Qiao, J;Chung, DH;,
Cancer Lett.
,PubMed ID 25264923
[TWIST1]
|
|
Twist: A Regulator of Epithelial-Mesenchymal Transition in Lung Fibrosis
,null,
PLoS ONE
,PubMed ID 19851501
[TWIST1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202920 | TWIST1 (Myc-DDK-tagged)-Human twist homolog 1 (Drosophila) (TWIST1) |
CNY 3,600.00 |
|
| RC202920L1 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC202920L2 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged |
CNY 6,000.00 |
|
| RC202920L3 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC202920L4 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged |
CNY 6,000.00 |
|
| RG202920 | TWIST1 (tGFP-tagged) - Human twist homolog 1 (Drosophila) (TWIST1) |
CNY 5,200.00 |
