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Calreticulin (CALR) (NM_004343) Human Tagged ORF Clone
CAT＃: RC203222
CALR (Myc-DDK-tagged)-Human calreticulin (CALR)
ORF Plasmid: tGFP
"NM_004343" in other vectors (6)
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CNY 5,488.00
| Cited in 6 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | cC1qR; CRT; HEL-S-99n; RO; SSA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203222 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTATCCGTGCCGCTGCTGCTCGGCCTCCTCGGCCTGGCCGTCGCCGAGCCTGCCGTCTACTTCA AGGAGCAGTTTCTGGACGGAGACGGGTGGACTTCCCGCTGGATCGAATCCAAACACAAGTCAGATTTTGG CAAATTCGTTCTCAGTTCCGGCAAGTTCTACGGTGACGAGGAGAAAGATAAAGGTTTGCAGACAAGCCAG GATGCACGCTTTTATGCTCTGTCGGCCAGTTTCGAGCCTTTCAGCAACAAAGGCCAGACGCTGGTGGTGC AGTTCACGGTGAAACATGAGCAGAACATCGACTGTGGGGGCGGCTATGTGAAGCTGTTTCCTAATAGTTT GGACCAGACAGACATGCACGGAGACTCAGAATACAACATCATGTTTGGTCCCGACATCTGTGGCCCTGGC ACCAAGAAGGTTCATGTCATCTTCAACTACAAGGGCAAGAACGTGCTGATCAACAAGGACATCCGTTGCA AGGATGATGAGTTTACACACCTGTACACACTGATTGTGCGGCCAGACAACACCTATGAGGTGAAGATTGA CAACAGCCAGGTGGAGTCCGGCTCCTTGGAAGACGATTGGGACTTCCTGCCACCCAAGAAGATAAAGGAT CCTGATGCTTCAAAACCGGAAGACTGGGATGAGCGGGCCAAGATCGATGATCCCACAGACTCCAAGCCTG AGGACTGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGACTGGGATGAAGAGAT GGACGGAGAGTGGGAACCCCCAGTGATTCAGAACCCTGAGTACAAGGGTGAGTGGAAGCCCCGGCAGATC GACAACCCAGATTACAAGGGCACTTGGATCCACCCAGAAATTGACAACCCCGAGTATTCTCCCGATCCCA GTATCTATGCCTATGATAACTTTGGCGTGCTGGGCCTGGACCTCTGGCAGGTCAAGTCTGGCACCATCTT TGACAACTTCCTCATCACCAACGATGAGGCATACGCTGAGGAGTTTGGCAACGAGACGTGGGGCGTAACA AAGGCAGCAGAGAAACAAATGAAGGACAAACAGGACGAGGAGCAGAGGCTTAAGGAGGAGGAAGAAGACA AGAAACGCAAAGAGGAGGAGGAGGCAGAGGACAAGGAGGATGATGAGGACAAAGATGAGGATGAGGAGGA TGAGGAGGACAAGGAGGAAGATGAGGAGGAAGATGTCCCCGGCCAGGCCAAGGACGAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203222 protein sequence
Red=Cloning site Green=Tags(s) MLLSVPLLLGLLGLAVAEPAVYFKEQFLDGDGWTSRWIESKHKSDFGKFVLSSGKFYGDEEKDKGLQTSQ DARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPG TKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKD PDASKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQI DNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVT KAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004343 |
| ORF Size | 1251 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004343.4 |
| RefSeq Size | 1929 bp |
| RefSeq ORF | 1254 bp |
| Locus ID | 811 |
| UniProt ID | P27797 |
| Domains | calreticulin |
| Protein Families | Druggable Genome, Secreted Protein, Transcription Factors |
| Protein Pathways | Antigen processing and presentation |
| MW | 48.1 kDa |
| Gene Summary | Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020] |
Citations (6)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Calreticulin Ins5 and Del52 mutations impair unfolded protein and oxidative stress responses in K562 cells expressing CALR mutants
,Salati, S;Genovese, E;Carretta, C;Zini, R;Bartalucci, N;Prudente, Z;Pennucci, V;Ruberti, S;Rossi, C;Rontauroli, S;Enzo, E;Calabresi, L;Balliu, M;Mannarelli, C;Bianchi, E;Guglielmelli, P;Tagliafico, E;Vannucchi, AM;Manfredini, R;,
Sci Rep
,PubMed ID 31332222
[Calreticulin]
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Mechanistic Understanding of N-Glycosylation in Ebola Virus Glycoprotein Maturation and Function
,Wang, B;Wang, Y;Frabutt, DA;Zhang, X;Yao, X;Hu, D;Zhang, Z;Liu, C;Zheng, S;Xiang, SH;Zheng, YH;,
J. Biol. Chem
,PubMed ID 28196864
[Calreticulin]
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Heterozygous NPR2 mutations cause disproportionate short stature, similar to Léri-Weill dyschondrosteosis
,Hisado-Oliva, A;Garre-Vázquez, AI;Santaolalla-Caballero, F;Belinchón, A;Barreda-Bonis, AC;Vasques, GA;Ramirez, J;Luzuriaga, C;Carlone, G;González-Casado, I;Benito-Sanz, S;Jorge, AA;Campos-Barros, A;Heath, KE;,
J. Clin. Endocrinol. Metab.
,PubMed ID 26075495
[Calreticulin]
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Rho GTPases RhoA and Rac1 Mediate Effects of Dietary Folate on Metastatic Potential of A549 Cancer Cells through the Control of Cofilin Phosphorylation
,Oleinik, NV;Helke, KL;Kistner-Griffin, E;Krupenko, NI;Krupenko, SA;,
J. Biol. Chem.
,PubMed ID 25086046
[Calreticulin]
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Proteomic Assessment Shows That Many Endoplasmic Reticulum (ER)-resident Proteins Are Targeted by Nϵ-Lysine Acetylation in the Lumen of the Organelle and Predicts Broad Biological Impact *
,null,
The Journal of Biological Chemistry
,PubMed ID 22628546
[Calreticulin]
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A Point Mutation in the Amino Terminus of TLR7 Abolishes Signaling without Affecting Ligand Binding
,Carlo Iavarone, Katrin Ramsauer, Andriy V. Kubarenko, Jason C. Debasitis, Igor Leykin, Alexander N. R. Weber, Owen M. Siggs, Bruce Beutler, Pu Zhang, Gillis Otten, Ugo D’Oro, Nicholas M. Valiante, M. Lamine Mbow, and Alberto Visintin,
J. Immunol., Apr 2011; 186: 4213 - 4222
[Calreticulin]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203222L1 | Lenti ORF clone of Human calreticulin (CALR), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203222L2 | Lenti ORF clone of Human calreticulin (CALR), mGFP tagged |
CNY 7,888.00 |
|
| RC203222L3 | Lenti ORF clone of Human calreticulin (CALR), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203222L4 | Lenti ORF clone of Human calreticulin (CALR), mGFP tagged |
CNY 7,888.00 |
|
| RG203222 | CALR (tGFP-tagged) - Human calreticulin (CALR) |
CNY 7,088.00 |
|
| SC320287 | CALR (untagged)-Human calreticulin (CALR) |
CNY 5,488.00 |
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