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Pellino 2 (PELI2) (NM_021255) Human Tagged ORF Clone
CAT＃: RC203409
PELI2 (Myc-DDK-tagged)-Human pellino homolog 2 (Drosophila) (PELI2)
ORF Plasmid: tGFP
"NM_021255" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203409 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTCCCCTGGCCAGGAGGAACACTGCGCCCCCAATAAGGAGCCAGTGAAATACGGGGAGCTGGTGG TGCTCGGGTACAATGGTGCTTTACCCAATGGAGATAGAGGACGGAGGAAAAGTAGATTTGCCCTCTACAA GCGGCCCAAGGCAAATGGTGTCAAACCCAGCACCGTCCATGTGATATCCACGCCCCAGGCATCCAAGGCT ATCAGCTGCAAAGGTCAACACAGTATATCCTACACTTTGTCAAGGAATCAGACTGTGGTGGTGGAGTACA CACATGATAAGGATACGGATATGTTTCAGGTGGGCAGATCAACAGAAAGCCCTATCGACTTCGTTGTCAC AGACACGATTTCTGGCAGCCAGAACACGGACGAAGCCCAGATCACACAGAGCACCATATCCAGGTTCGCC TGCAGGATCGTGTGCGACAGGAATGAACCTTACACAGCACGGATATTCGCCGCCGGATTTGACTCTTCCA AAAACATATTTCTTGGAGAAAAGGCAGCAAAGTGGAAAAACCCCGACGGCCACATGGATGGGCTCACTAC TAATGGCGTCCTGGTGATGCATCCACGAGGGGGCTTCACCGAGGAGTCCCAGCCCGGGGTCTGGCGCGAG ATCTCTGTCTGTGGAGATGTGTACACCTTGCGAGAAACCAGGTCGGCCCAGCAACGAGGAAAGCTGGTGG AAAGTGAGACCAACGTCCTGCAGGACGGCTCCCTCATTGACCTGTGTGGGGCCACTCTCCTCTGGAGAAC AGCAGATGGGCTTTTTCATACTCCAACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATTAACGCCGCC CGGCCTCAGTGTCCTGTGGGGCTCAACACCCTGGCCTTCCCCAGCATCAACAGGAAAGAGGTGGTGGAGG AGAAGCAGCCCTGGGCATATCTCAGTTGTGGCCACGTGCACGGGTACCACAACTGGGGCCATCGGAGTGA CACGGAGGCCAACGAGAGGGAGTGTCCCATGTGCAGGACTGTGGGCCCCTATGTGCCTCTCTGGCTTGGC TGTGAGGCAGGATTTTATGTAGACGCAGGACCGCCAACTCATGCTTTCACTCCCTGTGGACACGTGTGCT CGGAGAAGTCTGCAAAATACTGGTCTCAGATCCCGTTGCCTCATGGAACTCATGCATTTCACGCTGCTTG CCCTTTCTGTGCTACACAGCTGGTTGGGGAGCAAAACTGCATCAAATTAATTTTCCAAGGTCCAATTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203409 protein sequence
Red=Cloning site Green=Tags(s) MFSPGQEEHCAPNKEPVKYGELVVLGYNGALPNGDRGRRKSRFALYKRPKANGVKPSTVHVISTPQASKA ISCKGQHSISYTLSRNQTVVVEYTHDKDTDMFQVGRSTESPIDFVVTDTISGSQNTDEAQITQSTISRFA CRIVCDRNEPYTARIFAAGFDSSKNIFLGEKAAKWKNPDGHMDGLTTNGVLVMHPRGGFTEESQPGVWRE ISVCGDVYTLRETRSAQQRGKLVESETNVLQDGSLIDLCGATLLWRTADGLFHTPTQKHIEALRQEINAA RPQCPVGLNTLAFPSINRKEVVEEKQPWAYLSCGHVHGYHNWGHRSDTEANERECPMCRTVGPYVPLWLG CEAGFYVDAGPPTHAFTPCGHVCSEKSAKYWSQIPLPHGTHAFHAACPFCATQLVGEQNCIKLIFQGPID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021255 |
| ORF Size | 1260 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021255.3 |
| RefSeq Size | 5755 bp |
| RefSeq ORF | 1263 bp |
| Locus ID | 57161 |
| UniProt ID | Q9HAT8 |
| Domains | Pellino |
| Protein Families | Druggable Genome |
| MW | 46.4 kDa |
| Gene Summary | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. May be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. Can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203409L1 | Lenti ORF clone of Human pellino homolog 2 (Drosophila) (PELI2), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203409L2 | Lenti ORF clone of Human pellino homolog 2 (Drosophila) (PELI2), mGFP tagged |
CNY 5,890.00 |
|
| RC203409L3 | Lenti ORF clone of Human pellino homolog 2 (Drosophila) (PELI2), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203409L4 | Lenti ORF clone of Human pellino homolog 2 (Drosophila) (PELI2), mGFP tagged |
CNY 5,890.00 |
|
| RG203409 | PELI2 (tGFP-tagged) - Human pellino homolog 2 (Drosophila) (PELI2) |
CNY 7,088.00 |
|
| SC319321 | PELI2 (untagged)-Human pellino homolog 2 (Drosophila) (PELI2) |
CNY 5,488.00 |
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