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5T4 (TPBG) (NM_006670) Human Tagged ORF Clone
CAT＃: RC203484
TPBG (Myc-DDK-tagged)-Human trophoblast glycoprotein (TPBG), transcript variant 1
ORF Plasmid: tGFP
"NM_006670" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5T4; 5T4AG; M6P1; WAIF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203484 representing NM_006670
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGGGGGTGCTCCCGGGGCCCCGCCGCCGGGGACGGGCGTCTGCGGCTGGCGCGACTAGCGCTGG TACTCCTGGGCTGGGTCTCCTCGTCTTCTCCCACCTCCTCGGCATCCTCCTTCTCCTCCTCGGCGCCGTT CCTGGCTTCCGCCGTGTCCGCCCAGCCCCCGCTGCCGGACCAGTGCCCCGCGCTGTGCGAGTGCTCCGAG GCAGCGCGCACAGTCAAGTGCGTTAACCGCAATCTGACCGAGGTGCCCACGGACCTGCCCGCCTACGTGC GCAACCTCTTCCTTACCGGCAACCAGCTGGCCGTGCTCCCTGCCGGCGCCTTCGCCCGCCGGCCGCCGCT GGCGGAGCTGGCCGCGCTCAACCTCAGCGGCAGCCGCCTGGACGAGGTGCGCGCGGGCGCCTTCGAGCAT CTGCCCAGCCTGCGCCAGCTCGACCTCAGCCACAACCCACTGGCCGACCTCAGTCCCTTCGCTTTCTCGG GCAGCAATGCCAGCGTCTCGGCCCCCAGTCCCCTTGTGGAACTGATCCTGAACCACATCGTGCCCCCTGA AGATGAGCGGCAGAACCGGAGCTTCGAGGGCATGGTGGTGGCGGCCCTGCTGGCGGGCCGTGCACTGCAG GGGCTCCGCCGCTTGGAGCTGGCCAGCAACCACTTCCTTTACCTGCCGCGGGATGTGCTGGCCCAACTGC CCAGCCTCAGGCACCTGGACTTAAGTAATAATTCGCTGGTGAGCCTGACCTACGTGTCCTTCCGCAACCT GACACATCTAGAAAGCCTCCACCTGGAGGACAATGCCCTCAAGGTCCTTCACAATGGCACCCTGGCTGAG TTGCAAGGTCTACCCCACATTAGGGTTTTCCTGGACAACAATCCCTGGGTCTGCGACTGCCACATGGCAG ACATGGTGACCTGGCTCAAGGAAACAGAGGTAGTGCAGGGCAAAGACCGGCTCACCTGTGCATATCCGGA AAAAATGAGGAATCGGGTCCTCTTGGAACTCAACAGTGCTGACCTGGACTGTGACCCGATTCTTCCCCCA TCCCTGCAAACCTCTTATGTCTTCCTGGGTATTGTTTTAGCCCTGATAGGCGCTATTTTCCTCCTGGTTT TGTATTTGAACCGCAAGGGGATAAAAAAGTGGATGCATAACATCAGAGATGCCTGCAGGGATCACATGGA AGGGTATCATTACAGATATGAAATCAATGCGGACCCCAGATTAACAAACCTCAGTTCTAACTCGGATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203484 representing NM_006670
Red=Cloning site Green=Tags(s) MPGGCSRGPAAGDGRLRLARLALVLLGWVSSSSPTSSASSFSSSAPFLASAVSAQPPLPDQCPALCECSE AARTVKCVNRNLTEVPTDLPAYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRAGAFEH LPSLRQLDLSHNPLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQ GLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAE LQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPP SLQTSYVFLGIVLALIGAIFLLVLYLNRKGIKKWMHNIRDACRDHMEGYHYRYEINADPRLTNLSSNSDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006670 |
| ORF Size | 1260 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006670.1 |
| RefSeq Size | 2379 bp |
| RefSeq ORF | 1263 bp |
| Locus ID | 7162 |
| UniProt ID | Q13641 |
| Domains | LRRNT, LRRCT, LRR, LRR_TYP |
| Protein Families | Transmembrane |
| MW | 45.9 kDa |
| Gene Summary | This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203484L1 | Lenti ORF clone of Human trophoblast glycoprotein (TPBG), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203484L2 | Lenti ORF clone of Human trophoblast glycoprotein (TPBG), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC203484L3 | Lenti ORF clone of Human trophoblast glycoprotein (TPBG), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203484L4 | Lenti ORF clone of Human trophoblast glycoprotein (TPBG), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG203484 | TPBG (tGFP-tagged) - Human trophoblast glycoprotein (TPBG), transcript variant 1 |
CNY 7,088.00 |
|
| SC115948 | TPBG (untagged)-Human trophoblast glycoprotein (TPBG), transcript variant 1 |
CNY 5,488.00 |
