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C14orf169 (NM_024644) Human Tagged ORF Clone
CAT＃: RC203678
C14orf169 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 169 (C14orf169)
ORF Plasmid: tGFP
"NM_024644" in other vectors (4)
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CNY 4,776.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C14orf169; hsNO66; JMJD9; MAPJD; NO66; ROX; URLC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203678 representing NM_024644
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGGCTCCAGGCCAGTGCAGGGCCGTTGAGGCGCGGGCGGCCGAAGCGCCGGCGCAAGCCCCAGC CACACAGCGGGTCGGTCCTGGCCCTGCCCTTGAGGTCCAGGAAGATACGAAAGCAGCTGCGAAGTGTTGT ATCCCGCATGGCAGCGCTGAGGACGCAGACGCTGCCTAGCGAGAACTCGGAGGAATCGAGGGTGGAGTCG ACGGCCGACGACCTGGGGGACGCGCTACCCGGTGGGGCGGCGGTGGCGGCCGTCCCGGACGCAGCCCGGC GAGAGCCATACGGCCACCTGGGGCCCGCAGAGCTGCTGGAGGCCTCGCCCGCCGCGCGCTCCCTGCAGAC CCCGTCGGCGCGCCTGGTGCCCGCTTCCGCGCCGCCCGCGCGCCTGGTGGAGGTGCCCGCCGCGCCGGTC CGGGTGGTGGAGACCTCGGCCCTGCTGTGCACCGCGCAACACTTAGCGGCCGTCCAGTCGTCCGGGGCCC CTGCGACGGCGTCGGGGCCGCAGGTGGATAACACGGGTGGGGAGCCGGCCTGGGACTCCCCGCTGCGGCG CGTCTTGGCCGAGCTGAACCGCATCCCCAGCAGCCGGCGGCGAGCGGCCCGCCTCTTTGAGTGGCTCATC GCGCCCATGCCGCCAGATCACTTTTACCGGCGCCTATGGGAGCGCGAGGCGGTGCTGGTGCGGCGGCAGG ACCACACCTACTACCAGGGACTTTTCTCTACCGCTGACCTGGATTCGATGCTGCGCAACGAGGAGGTGCA GTTCGGCCAGCATTTGGACGCCGCTCGCTACATCAACGGACGACGCGAGACCCTGAACCCACCCGGCCGC GCGCTGCCCGCCGCCGCGTGGTCCCTGTACCAGGCCGGCTGCTCCCTGCGTCTCCTCTGTCCGCAGGCTT TCTCTACTACTGTGTGGCAGTTTTTGGCTGTGCTTCAAGAGCAGTTTGGAAGCATGGCAGGCTCCAACGT TTACCTCACGCCCCCTAACTCGCAGGGCTTTGCCCCCCACTACGACGACATCGAGGCCTTCGTGCTGCAG CTGGAAGGTAGGAAACTCTGGCGTGTATACCGACCCCGAGTCCCAACCGAGGAACTGGCTCTGACATCCA GCCCCAACTTCAGTCAGGACGACCTCGGTGAGCCGGTGCTGCAGACCGTGCTGGAACCTGGAGATTTGCT GTATTTTCCTCGGGGCTTCATTCACCAAGCTGAATGCCAGGATGGAGTCCACTCTCTGCACCTCACCTTG TCCACGTACCAGCGCAATACCTGGGGTGACTTCTTAGAGGCCATACTGCCTCTGGCAGTGCAGGCTGCAA TGGAAGAAAATGTGGAGTTTCGGAGGGGTCTGCCCCGAGACTTCATGGATTACATGGGGGCCCAGCATTC AGATTCTAAGGATCCGCGAAGAACCGCTTTCATGGAGAAGGTGCGGGTCTTGGTTGCCCGCCTGGGACAC TTTGCTCCTGTTGATGCTGTGGCCGACCAGCGAGCCAAAGACTTCATTCACGATTCTCTGCCCCCTGTTT TGACTGATAGGGAGAGGGCACTAAGTGTTTACGGGCTTCCAATTCGCTGGGAGGCTGGAGAACCTGTAAA CGTGGGGGCCCAGTTGACAACAGAAACAGAAGTCCATATGCTTCAGGATGGGATAGCTCGGCTGGTGGGT GAGGGGGGCCATTTGTTTCTCTATTACACAGTGGAAAACTCCCGTGTGTATCATCTGGAAGAACCCAAGT GCTTGGAAATATACCCCCAGCAAGCTGATGCCATGGAACTGTTGCTTGGTTCTTATCCAGAGTTTGTGAG AGTGGGGGACCTGCCCTGTGACAGTGTGGAGGACCAGCTGTCCTTGGCAACCACGTTGTATGATAAGGGG CTGCTGCTCACTAAGATGCCTCTAGCCCTAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203678 representing NM_024644
Red=Cloning site Green=Tags(s) MDGLQASAGPLRRGRPKRRRKPQPHSGSVLALPLRSRKIRKQLRSVVSRMAALRTQTLPSENSEESRVES TADDLGDALPGGAAVAAVPDAARREPYGHLGPAELLEASPAARSLQTPSARLVPASAPPARLVEVPAAPV RVVETSALLCTAQHLAAVQSSGAPATASGPQVDNTGGEPAWDSPLRRVLAELNRIPSSRRRAARLFEWLI APMPPDHFYRRLWEREAVLVRRQDHTYYQGLFSTADLDSMLRNEEVQFGQHLDAARYINGRRETLNPPGR ALPAAAWSLYQAGCSLRLLCPQAFSTTVWQFLAVLQEQFGSMAGSNVYLTPPNSQGFAPHYDDIEAFVLQ LEGRKLWRVYRPRVPTEELALTSSPNFSQDDLGEPVLQTVLEPGDLLYFPRGFIHQAECQDGVHSLHLTL STYQRNTWGDFLEAILPLAVQAAMEENVEFRRGLPRDFMDYMGAQHSDSKDPRRTAFMEKVRVLVARLGH FAPVDAVADQRAKDFIHDSLPPVLTDRERALSVYGLPIRWEAGEPVNVGAQLTTETEVHMLQDGIARLVG EGGHLFLYYTVENSRVYHLEEPKCLEIYPQQADAMELLLGSYPEFVRVGDLPCDSVEDQLSLATTLYDKG LLLTKMPLALN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024644 |
| ORF Size | 1923 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024644.5 |
| RefSeq Size | 2463 bp |
| RefSeq ORF | 1926 bp |
| Locus ID | 79697 |
| UniProt ID | Q9H6W3 |
| Protein Families | Druggable Genome |
| MW | 71.1 kDa |
| Gene Summary | Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203678L3 | Lenti-ORF clone of C14orf169 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 169 (C14orf169) |
CNY 5,890.00 |
|
| RC203678L4 | Lenti-ORF clone of C14orf169 (mGFP-tagged)-Human chromosome 14 open reading frame 169 (C14orf169) |
CNY 5,890.00 |
|
| RG203678 | C14orf169 (tGFP-tagged) - Human chromosome 14 open reading frame 169 (C14orf169) |
CNY 4,370.00 |
|
| SC122963 | C14orf169 (untagged)-Human chromosome 14 open reading frame 169 (C14orf169) |
CNY 4,784.00 |
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