ALIX (PDCD6IP) (NM_013374) Human Tagged ORF Clone
CAT#: RC203735
PDCD6IP (Myc-DDK-tagged)-Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1
ORF Plasmid: tGFP
"NM_013374" in other vectors (7)
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CNY 6,360.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AIP1; ALIX; DRIP4; HP95 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203735 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACATTCATCTCGGTGCAGCTGAAAAAGACCTCAGAGGTGGACCTGGCCAAGCCGCTGGTGAAGT TCATCCAGCAGACTTACCCAAGCGGCGGGGAAGAGCAGGCCCAGTACTGCCGCGCGGCGGAGGAGCTCAG CAAGCTGCGCCGCGCCGCAGTCGGTCGTCCGCTGGACAAGCACGAGGGCGCGCTCGAGACGCTCCTGAGA TATTATGATCAGATTTGTTCTATTGAACCCAAATTCCCATTTTCTGAAAATCAGATCTGCTTGACATTTA CCTGGAAGGATGCTTTCGATAAAGGTTCACTTTTTGGAGGCTCTGTAAAACTGGCTCTTGCAAGCTTAGG ATATGAAAAGAGCTGTGTGTTGTTCAATTGTGCAGCCTTAGCTAGCCAAATTGCAGCAGAACAGAACCTG GATAATGATGAAGGATTGAAAATCGCTGCTAAACATTACCAGTTTGCTAGTGGTGCCTTTTTACATATTA AAGAGACGGTTTTATCTGCCTTAAGTCGAGAGCCGACCGTGGACATATCTCCAGATACTGTTGGGACCCT CAGTCTTATTATGCTGGCACAGGCTCAAGAAGTATTTTTTTTAAAAGCCACAAGAGATAAAATGAAAGAT GCCATCATAGCTAAATTGGCTAATCAGGCTGCAGATTATTTTGGTGATGCTTTCAAACAGTGTCAATACA AAGATACTCTCCCCAAGGAGGTGTTCCCTGTCTTGGCTGCAAAGCACTGTATCATGCAGGCCAATGCTGA GTACCATCAGTCTATCCTGGCAAAACAGCAGAAGAAATTTGGAGAAGAAATTGCAAGGTTACAGCATGCA GCAGAACTGATTAAAACAGTGGCATCTCGCTATGATGAATATGTTAATGTGAAGGATTTTTCTGACAAAA TCAATCGTGCCCTTGCTGCAGCAAAGAAGGATAATGACTTCATTTATCATGATCGAGTTCCAGACCTTAA AGATCTAGATCCTATTGGCAAAGCCACACTTGTGAAATCTACCCCGGTCAATGTACCCATCAGTCAGAAA TTTACTGATCTGTTTGAGAAGATGGTTCCCGTGTCAGTACAGCAGTCTTTGGCTGCCTATAATCAGAGGA AAGCCGATTTGGTTAACAGATCAATTGCTCAGATGAGAGAAGCCACCACTTTGGCAAATGGGGTGCTAGC TTCCCTTAATCTTCCAGCAGCAATTGAAGATGTGTCTGGAGACACTGTACCTCAGTCTATATTGACTAAA TCCAGATCTGTGATTGAACAGGGAGGCATCCAGACTGTTGATCAGTTGATTAAAGAACTGCCTGAATTAC TGCAACGAAATAGAGAAATCCTAGATGAGTCATTAAGGTTGTTGGATGAAGAAGAAGCAACCGATAATGA TTTAAGAGCAAAATTTAAGGAACGTTGGCAAAGGACACCATCCAATGAACTGTATAAGCCTTTAAGAGCA GAGGGAACCAACTTCAGAACAGTTTTAGATAAAGCTGTGCAGGCAGATGGACAAGTGAAAGAATGTTACC AGTCTCATCGTGACACCATCGTGCTTTTGTGTAAGCCAGAGCCTGAGCTGAATGCTGCCATCCCTTCTGC TAATCCAGCAAAGACCATGCAGGGCAGTGAGGTTGTAAATGTCTTAAAATCCTTATTGTCAAATCTTGAT GAAGTAAAGAAGGAAAGAGAGGGTCTGGAGAATGACTTGAAATCTGTGAATTTTGACATGACAAGCAAGT TTTTGACAGCCCTGGCTCAAGATGGTGTGATAAATGAAGAAGCTCTTTCTGTTACTGAACTAGATCGAGT CTATGGAGGTCTTACAACTAAAGTCCAAGAATCTCTAAAGAAACAGGAGGGACTTCTTAAAAATATTCAG GTCTCACATCAGGAATTTTCAAAAATGAAACAATCTAATAATGAAGCTAACTTAAGAGAAGAAGTTTTGA AGAATTTAGCTACTGCATATGACAACTTTGTTGAACTTGTAGCTAATTTGAAGGAAGGCACAAAGTTTTA CAATGAGTTGACTGAAATCCTGGTCAGGTTCCAGAACAAATGCAGTGATATAGTTTTTGCACGGAAGACA GAAAGAGATGAACTCTTAAAGGACTTGCAACAAAGCATTGCCAGAGAACCTAGTGCTCCTTCAATTCCTA CACCTGCGTATCAGTCCTCACCAGCAGGAGGACATGCACCAACTCCTCCAACTCCAGCGCCAAGAACCAT GCCGCCTACTAAGCCCCAGCCCCCAGCCAGGCCTCCACCACCTGTGCTTCCAGCAAATCGAGCTCCTTCT GCTACTGCTCCATCTCCAGTGGGGGCTGGGACTGCTGCGCCAGCTCCATCACAAACGCCTGGCTCAGCTC CTCCTCCACAGGCGCAGGGACCACCCTATCCCACCTATCCAGGATATCCTGGGTATTGCCAAATGCCCAT GCCCATGGGCTATAATCCTTATGCGTATGGCCAGTATAATATGCCATATCCACCAGTGTATCACCAGAGT CCTGGACAGGCTCCATACCCGGGACCCCAGCAGCCTTCATACCCCTTCCCTCAGCCCCCACAGCAGTCTT ACTATCCACAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203735 protein sequence
Red=Cloning site Green=Tags(s) MATFISVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRAAVGRPLDKHEGALETLLR YYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNL DNDEGLKIAAKHYQFASGAFLHIKETVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKD AIIAKLANQAADYFGDAFKQCQYKDTLPKEVFPVLAAKHCIMQANAEYHQSILAKQQKKFGEEIARLQHA AELIKTVASRYDEYVNVKDFSDKINRALAAAKKDNDFIYHDRVPDLKDLDPIGKATLVKSTPVNVPISQK FTDLFEKMVPVSVQQSLAAYNQRKADLVNRSIAQMREATTLANGVLASLNLPAAIEDVSGDTVPQSILTK SRSVIEQGGIQTVDQLIKELPELLQRNREILDESLRLLDEEEATDNDLRAKFKERWQRTPSNELYKPLRA EGTNFRTVLDKAVQADGQVKECYQSHRDTIVLLCKPEPELNAAIPSANPAKTMQGSEVVNVLKSLLSNLD EVKKEREGLENDLKSVNFDMTSKFLTALAQDGVINEEALSVTELDRVYGGLTTKVQESLKKQEGLLKNIQ VSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKT ERDELLKDLQQSIAREPSAPSIPTPAYQSSPAGGHAPTPPTPAPRTMPPTKPQPPARPPPPVLPANRAPS ATAPSPVGAGTAAPAPSQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQS PGQAPYPGPQQPSYPFPQPPQQSYYPQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013374 |
ORF Size | 2604 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013374.6 |
RefSeq Size | 5972 bp |
RefSeq ORF | 2607 bp |
Locus ID | 10015 |
UniProt ID | Q8WUM4 |
Domains | BRO1 |
Protein Families | Druggable Genome |
Protein Pathways | Endocytosis |
MW | 96 kDa |
Gene Summary | This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Lysosomal N-acetyltransferase interacts with ALIX and is detected in extracellular vesicles
,Fedele, AO;Isenmann, S;Kamei, M;Snel, MF;Trim, PJ;Proud, CG;Hopwood, JJ;,
Biochim. Biophys. Acta
,PubMed ID 29981367
[ALIX]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203735L1 | Lenti ORF clone of Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1, Myc-DDK-tagged |
CNY 8,760.00 |
|
RC203735L2 | Lenti ORF clone of Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC203735L3 | Lenti ORF clone of Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203735L4 | Lenti ORF clone of Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1, mGFP tagged |
CNY 8,760.00 |
|
RG203735 | PDCD6IP (tGFP-tagged) - Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1 |
CNY 7,960.00 |
|
SC115270 | PDCD6IP (untagged)-Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1 |
CNY 6,368.00 |
|
SC323909 | PDCD6IP (untagged)-Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1 |
CNY 6,368.00 |