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Dystrobrevin beta (DTNB) (NM_183361) Human Tagged ORF Clone
CAT＃: RC203798
DTNB (Myc-DDK-tagged)-Human dystrobrevin, beta (DTNB), transcript variant 5
ORF Plasmid: tGFP
"NM_183361" in other vectors (6)
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CNY 4,168.00
CNY 4,560.00
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CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203798 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGAGGAAAGTGGGAACAAGCGGAAGACCATGGCAGAGAAGAGGCAGCTGTTCATAGAAATGCGTG CTCAGAATTTTGATGTCATACGACTATCAACTTACAGAACAGCCTGCAAATTACGATTTGTACAAAAACG ATGCAACCTTCATCTTGTTGATATCTGGAACATGATTGAAGCCTTCCGAGACAATGGCCTTAATACACTG GACCATACCACCGAGATCAGTGTGTCCCGCCTCGAAACTGTCATCTCCTCCATCTACTATCAGTTGAACA AGCGCCTTCCTTCTACTCACCAAATTAGTGTGGAACAATCTATCAGCCTCCTCCTCAACTTTATGATTGC TGCATATGACAGTGAGGGCCGAGGCAAGTTGACGGTATTTTCAGTTAAAGCTATGTTAGCAACCATGTGT GGTGGAAAAATGCTGGACAAATTGAGATATGTTTTCTCCCAGATGTCAGATTCCAATGGCTTAATGATAT TTAGCAAGTTTGACCAGTTTCTGAAGGAAGTTCTGAAGCTCCCAACAGCTGTCTTTGAAGGGCCATCTTT TGGTTACACAGAGCACTCAGTCCGCACCTGTTTTCCACAGCAGAGAAAGATAATGCTAAATATGTTTTTA GACACAATGATGGCTGACCCTCCTCCCCAGTGCCTTGTCTGGCTACCTCTCATGCACAGGCTTGCCCATG TTGAGAATGTCTTCCATCCCGTGGAGTGCTCCTACTGCCGATGTGAGAGTATGATGGGTTTCCGGTACCG ATGCCAGCAGTGCCACAACTATCAGCTCTGCCAGAATTGCTTTTGGCGTGGCCATGCCGGCGGCCCTCAC AGCAACCAGCACCAGATGAAGGAGCATTCCTCTTGGAAATCTCCTGCAAAGAAGCTGAGCCATGCAATTA GTAAATCTTTGGGGTGTGTACCCACGAGAGAACCCCCGCATCCTGTTTTTCCTGAGCAACCAGAGAAACC ACTTGACCTTGCACATATAGTTCCTCCTCGCCCTCTGACTAATATGAATGACACCATGGTTAGCCACATG TCCTCTGGAGTGCCCACTCCCACCAAGAGTGTTCTGGACAGTCCTAGCCGACTGGATGAGGAACACCGTC TTATAGCTCGCTATGCTGCCCGGCTGGCTGCAGAAGCAGGAAACGTGACTCGTCCTCCCACTGACTTGAG CTTTAACTTTGATGCCAACAAACAACAAAGACAGCTTATTGCAGAACTGGAAAACAAAAACAGAGAGATC CTGCAGGAGATTCAGCGTCTCCGCCTGGAACACGAGCAGGCCTCCCAGCCCACCCCTGAGAAGGCACAGC AGAACCCCACGCTGCTGGCAGAGCTGCGGCTGCTGAGGCAAAGGAAGGATGAACTGGAGCAGAGGATGTC GGCCCTGCAGGAGAGCAGGCGGGAGCTGATGGTCCAGCTGGAAGAGCTGATGAAGTTGCTGAAGGCTCAG GCCACAGGGTCACCACATACATCGCCCACCCATGGAGGCGGCCGGCCAATGCCCATGCCAGTGCGCTCCA CGTCTGCCGGCTCCACCCCCACCCACTGTCCGCAGGACTCGCTGAGCGGAGTCGGGGGAGACGTGCAGGA GGCCTTCGCACAAGCAGAGGAAGGTGCAGAGGAAGAAGAAGAGAAGATGCAGAATGGGAAAGACAGAGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203798 protein sequence
Red=Cloning site Green=Tags(s) MIEESGNKRKTMAEKRQLFIEMRAQNFDVIRLSTYRTACKLRFVQKRCNLHLVDIWNMIEAFRDNGLNTL DHTTEISVSRLETVISSIYYQLNKRLPSTHQISVEQSISLLLNFMIAAYDSEGRGKLTVFSVKAMLATMC GGKMLDKLRYVFSQMSDSNGLMIFSKFDQFLKEVLKLPTAVFEGPSFGYTEHSVRTCFPQQRKIMLNMFL DTMMADPPPQCLVWLPLMHRLAHVENVFHPVECSYCRCESMMGFRYRCQQCHNYQLCQNCFWRGHAGGPH SNQHQMKEHSSWKSPAKKLSHAISKSLGCVPTREPPHPVFPEQPEKPLDLAHIVPPRPLTNMNDTMVSHM SSGVPTPTKSVLDSPSRLDEEHRLIARYAARLAAEAGNVTRPPTDLSFNFDANKQQRQLIAELENKNREI LQEIQRLRLEHEQASQPTPEKAQQNPTLLAELRLLRQRKDELEQRMSALQESRRELMVQLEELMKLLKAQ ATGSPHTSPTHGGGRPMPMPVRSTSAGSTPTHCPQDSLSGVGGDVQEAFAQAEEGAEEEEEKMQNGKDRG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183361 |
| ORF Size | 1680 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_183361.2, NP_899205.1 |
| RefSeq Size | 2328 bp |
| RefSeq ORF | 1683 bp |
| Locus ID | 1838 |
| UniProt ID | O60941 |
| MW | 63.8 kDa |
| Gene Summary | This gene encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203798L1 | Lenti ORF clone of Human dystrobrevin, beta (DTNB), transcript variant 5, Myc-DDK-tagged |
CNY 6,568.00 |
|
| RC203798L2 | Lenti ORF clone of Human dystrobrevin, beta (DTNB), transcript variant 5, mGFP tagged |
CNY 6,460.00 |
|
| RC203798L3 | Lenti ORF clone of Human dystrobrevin, beta (DTNB), transcript variant 5, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203798L4 | Lenti ORF clone of Human dystrobrevin, beta (DTNB), transcript variant 5, mGFP tagged |
CNY 6,460.00 |
|
| RG203798 | DTNB (tGFP-tagged) - Human dystrobrevin, beta (DTNB), transcript variant 5 |
CNY 5,768.00 |
|
| SC310268 | DTNB (untagged)-Human dystrobrevin, beta (DTNB), transcript variant 5 |
CNY 7,220.00 |
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