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GLO1 (NM_006708) Human Tagged ORF Clone
CAT＃: RC203826
GLO1 (Myc-DDK-tagged)-Human glyoxalase I (GLO1)
ORF Plasmid: tGFP
"NM_006708" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GLOD1; GLYI; HEL-S-74 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203826 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTCAGTTACTGCTCCGACGCGG ACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTACGAGTGAAGGATCCTAAGAAGTCACTGGA TTTTTATACTAGAGTTCTTGGAATGACGCTAATCCAAAAATGTGATTTTCCCATTATGAAGTTTTCACTC TACTTCTTGGCTTATGAGGATAAAAATGACATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCT CCAGAAAAGCTACACTTGAGCTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAA TGGCAATTCAGACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGTGCTTGTAAA AGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAAATGAAAGGCCTGGCATTTA TTCAAGATCCTGATGGCTACTGGATTGAAATTTTGAATCCTAACAAAATGGCAACCTTAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203826 protein sequence
Red=Cloning site Green=Tags(s) MAEPQPPSGGLTDEAALSYCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSL YFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACK RFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006708 |
| ORF Size | 552 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006708.3 |
| RefSeq Size | 2071 bp |
| RefSeq ORF | 555 bp |
| Locus ID | 2739 |
| UniProt ID | Q04760 |
| Domains | Glyoxalase |
| Protein Pathways | Pyruvate metabolism |
| MW | 20.8 kDa |
| Gene Summary | The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Glyoxalase 1 sustains the metastatic phenotype of prostate cancer cells via EMT control
,Antognelli, C;Cecchetti, R;Riuzzi, F;Peirce, MJ;Talesa, VN;,
J. Cell. Mol. Med.
,PubMed ID 29504694
[GLO1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203826L1 | Lenti ORF clone of Human glyoxalase I (GLO1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203826L2 | Lenti ORF clone of Human glyoxalase I (GLO1), mGFP tagged |
CNY 5,890.00 |
|
| RC203826L3 | Lenti ORF clone of Human glyoxalase I (GLO1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203826L4 | Lenti ORF clone of Human glyoxalase I (GLO1), mGFP tagged |
CNY 4,800.00 |
|
| RG203826 | GLO1 (tGFP-tagged) - Human glyoxalase I (GLO1) |
CNY 4,000.00 |
|
| SC115924 | GLO1 (untagged)-Human glyoxalase I (GLO1) |
CNY 2,400.00 |
