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PCGF6 (NM_001011663) Human Tagged ORF Clone
CAT＃: RC203882
PCGF6 (Myc-DDK-tagged)-Human polycomb group ring finger 6 (PCGF6), transcript variant 1
ORF Plasmid: tGFP
"NM_001011663" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MBLR; RNF134 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203882 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGGTCGCGGTGGTGACGGCGGGCAGCGTAGGCGCTGCCAAAACCGAGGGAGCTGCAGCCTTGC CGCCTCCGCCTCCGCCTCCTGTCTCCCCGCCCGCCCTCACCCCTGCACCCGCAGCGGGTGAGGAGGGACC GGCGCCTCTGTCTGAGACGGGGGCTCCCGGCTGCTCCGGCTCCCGGCCCCCTGAGCTGGAGCCGGAGCGC AGCCTGGGCCGCTTCAGAGGCCGCTTCGAGGACGAGGACGAGGAGTTGGAAGAAGAAGAGGAGCTGGAGG AGGAAGAAGAGGAGGAGGAGGAGGACATGAGTCACTTCTCGTTGAGGCTGGAGGGAGGCCGGCAGGACTC GGAGGACGAGGAGGAGCGCCTGATTAATCTCTCTGAGCTGACCCCATACATCTTGTGTTCCATTTGCAAA GGTTACTTAATAGATGCAACTACCATCACAGAATGTCTTCATACCTTTTGTAAAAGCTGCATCGTAAGAC ATTTTTACTACAGCAACAGATGTCCAAAATGCAATATAGTAGTACATCAGACACAACCTCTTTATAACAT AAGGTTGGACCGACAGTTACAAGACATAGTGTACAAATTAGTGATCAATCTAGAGGAAAGAGAAAAAAAG CAAATGCATGATTTCTATAAAGAAAGAGGTCTAGAAGTACCTAAACCTGCTGTTCCACAGCCAGTCCCTT CAAGCAAAGGAAGATCTAAAAAAGTCCTAGAATCAGTGTTTCGTATTCCACCTGAACTTGATATGTCTTT ATTACTGGAGTTCATTGGTGCTAATGAAGGCACGGGACATTTTAAGCCATTGGAAAAGAAGTTTGTTCGA GTTTCAGGAGAAGCAACTATTGGACATGTAGAAAAATTCCTCAGAAGAAAAATGGGTCTTGATCCAGCTT GTCAGGTAGATATAATCTGTGGTGATCACCTGTTGGAGCAGTATCAAACTCTAAGGGAAATCCGACGTGC AATAGGTGATGCAGCAATGCAGGATGGTCTGCTTGTCCTTCATTATGGTCTTGTGGTTTCTCCTCTGAAG ATAACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203882 protein sequence
Red=Cloning site Green=Tags(s) MEGVAVVTAGSVGAAKTEGAAALPPPPPPPVSPPALTPAPAAGEEGPAPLSETGAPGCSGSRPPELEPER SLGRFRGRFEDEDEELEEEEELEEEEEEEEEDMSHFSLRLEGGRQDSEDEEERLINLSELTPYILCSICK GYLIDATTITECLHTFCKSCIVRHFYYSNRCPKCNIVVHQTQPLYNIRLDRQLQDIVYKLVINLEEREKK QMHDFYKERGLEVPKPAVPQPVPSSKGRSKKVLESVFRIPPELDMSLLLEFIGANEGTGHFKPLEKKFVR VSGEATIGHVEKFLRRKMGLDPACQVDIICGDHLLEQYQTLREIRRAIGDAAMQDGLLVLHYGLVVSPLK IT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001011663 |
| ORF Size | 1056 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001011663.1, NP_001011663.1 |
| RefSeq Size | 2248 bp |
| RefSeq ORF | 1053 bp |
| Locus ID | 84108 |
| UniProt ID | Q9BYE7 |
| Protein Families | Transcription Factors |
| MW | 39.2 kDa |
| Gene Summary | The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203882L1 | Lenti ORF clone of Human polycomb group ring finger 6 (PCGF6), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203882L2 | Lenti ORF clone of Human polycomb group ring finger 6 (PCGF6), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC203882L3 | Lenti ORF clone of Human polycomb group ring finger 6 (PCGF6), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203882L4 | Lenti ORF clone of Human polycomb group ring finger 6 (PCGF6), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG203882 | PCGF6 (tGFP-tagged) - Human polycomb group ring finger 6 (PCGF6), transcript variant 1 |
CNY 7,088.00 |
|
| SC323726 | PCGF6 (untagged)-Human polycomb group ring finger 6 (PCGF6), transcript variant 1 |
CNY 5,488.00 |
