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GGCX (NM_000821) Human Tagged ORF Clone
CAT＃: RC204003
GGCX (Myc-DDK-tagged)-Human gamma-glutamyl carboxylase (GGCX), transcript variant 1
ORF Plasmid: tGFP
"NM_000821" in other vectors (5)
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CNY 5,552.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | VKCFD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204003 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGTCTGCCGGGTCCGCGCGGACCTCGCCCAGCTCAGATAAAGTACAGAAAGACAAGGCTGAAC TGATCTCAGGGCCCAGGCAGGACAGCCGAATAGGGAAACTCTTGGGTTTTGAGTGGACAGATTTGTCCAG TTGGCGGAGGCTGGTGACCCTGCTGAATCGACCAACGGACCCTGCAAGCTTAGCTGTCTTTCGTTTTCTT TTTGGGTTCTTGATGGTGCTAGACATTCCCCAGGAGCGGGGGCTCAGCTCTCTGGACCGGAAATACCTTG ATGGGCTGGATGTGTGCCGCTTCCCCTTGCTGGATGCCCTACGCCCACTGCCACTTGACTGGATGTATCT TGTCTACACCATCATGTTTCTGGGGGCACTGGGCATGATGCTGGGCCTGTGCTACCGGATAAGCTGTGTG TTATTCCTGCTGCCATACTGGTATGTGTTTCTCCTGGACAAGACATCATGGAACAACCACTCCTATCTGT ATGGGTTGTTGGCCTTTCAGCTAACATTCATGGATGCAAACCACTACTGGTCTGTGGACGGTCTGCTGAA TGCCCATAGGAGGAATGCCCACGTGCCCCTTTGGAACTATGCAGTGCTCCGTGGCCAGATCTTCATTGTG TACTTCATTGCGGGTGTGAAAAAGCTGGATGCAGACTGGGTTGAAGGCTATTCCATGGAATATTTGTCCC GGCACTGGCTCTTCAGTCCCTTCAAACTGCTGTTGTCTGAGGAGCTGACTAGCCTGCTGGTCGTGCACTG GGGTGGGCTGCTGCTTGACCTCTCAGCTGGTTTCCTGCTCTTTTTTGATGTCTCAAGATCCATTGGCCTG TTCTTTGTGTCCTACTTCCACTGCATGAATTCCCAGCTTTTCAGCATTGGTATGTTCTCCTACGTCATGC TGGCCAGCAGCCCTCTCTTCTGCTCCCCTGAGTGGCCTCGGAAGCTGGTGTCCTACTGCCCCCGAAGGTT GCAACAACTGTTGCCCCTCAAGGCAGCCCCTCAGCCCAGTGTTTCCTGTGTGTATAAGAGGAGCCGGGGC AAAAGTGGCCAGAAGCCAGGGCTGCGCCATCAGCTGGGAGCTGCCTTCACCCTGCTCTACCTCCTGGAGC AGCTATTCCTGCCCTATTCTCATTTTCTCACCCAGGGCTATAACAACTGGACAAATGGGCTGTATGGCTA TTCCTGGGACATGATGGTGCACTCCCGCTCCCACCAGCACGTGAAGATCACCTACCGTGATGGCCGCACT GGCGAACTGGGCTACCTTAACCCTGGGGTATTTACACAGAGTCGGCGATGGAAGGATCATGCAGACATGC TGAAGCAATATGCCACTTGCCTGAGCCGCCTGCTTCCCAAGTATAATGTCACTGAGCCCCAGATCTACTT TGATATTTGGGTCTCCATCAATGACCGCTTCCAGCAGAGGATTTTTGACCCTCGTGTGGACATCGTGCAG GCCGCTTGGTCACCCTTTCAGCGCACATCCTGGGTGCAACCACTCTTGATGGACCTGTCTCCCTGGAGGG CCAAGTTACAGGAAATCAAGAGCAGCCTAGACAACCACACTGAGGTGGTCTTCATTGCAGATTTCCCTGG ACTGCACTTGGAGAATTTTGTGAGTGAAGACCTGGGCAACACTAGCATCCAGCTGCTGCAGGGGGAAGTG ACTGTGGAGCTTGTGGCAGAACAGAAGAACCAGACTCTTCGAGAGGGAGAAAAAATGCAGTTGCCTGCTG GTGAGTACCATAAGGTGTATACGACATCACCTAGCCCTTCTTGCTACATGTACGTCTATGTCAACACTAC AGAGCTTGCACTGGAGCAAGACCTGGCATATCTGCAAGAATTAAAGGAAAAGGTGGAGAATGGAAGTGAA ACAGGGCCTCTACCCCCAGAGCTGCAGCCTCTGTTGGAAGGGGAAGTAAAAGGGGGCCCTGAGCCAACAC CTCTGGTTCAGACCTTTCTTAGACGCCAACAAAGGCTCCAGGAGATTGAACGCCGGCGAAATACTCCTTT CCATGAGCGATTCTTCCGCTTCTTGTTGCGAAAGCTCTATGTCTTTCGCCGCAGCTTCCTGATGACTTGT ATCTCACTTCGAAATCTGATATTAGGCCGTCCTTCCCTGGAGCAGCTGGCCCAGGAGGTGACTTATGCAA ACTTGAGACCCTTTGAGGCAGTTGGAGAACTGAATCCCTCAAACACGGATTCTTCACATTCTAATCCTCC TGAGTCAAATCCTGATCCTGTCCACTCAGAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204003 protein sequence
Red=Cloning site Green=Tags(s) MAVSAGSARTSPSSDKVQKDKAELISGPRQDSRIGKLLGFEWTDLSSWRRLVTLLNRPTDPASLAVFRFL FGFLMVLDIPQERGLSSLDRKYLDGLDVCRFPLLDALRPLPLDWMYLVYTIMFLGALGMMLGLCYRISCV LFLLPYWYVFLLDKTSWNNHSYLYGLLAFQLTFMDANHYWSVDGLLNAHRRNAHVPLWNYAVLRGQIFIV YFIAGVKKLDADWVEGYSMEYLSRHWLFSPFKLLLSEELTSLLVVHWGGLLLDLSAGFLLFFDVSRSIGL FFVSYFHCMNSQLFSIGMFSYVMLASSPLFCSPEWPRKLVSYCPRRLQQLLPLKAAPQPSVSCVYKRSRG KSGQKPGLRHQLGAAFTLLYLLEQLFLPYSHFLTQGYNNWTNGLYGYSWDMMVHSRSHQHVKITYRDGRT GELGYLNPGVFTQSRRWKDHADMLKQYATCLSRLLPKYNVTEPQIYFDIWVSINDRFQQRIFDPRVDIVQ AAWSPFQRTSWVQPLLMDLSPWRAKLQEIKSSLDNHTEVVFIADFPGLHLENFVSEDLGNTSIQLLQGEV TVELVAEQKNQTLREGEKMQLPAGEYHKVYTTSPSPSCYMYVYVNTTELALEQDLAYLQELKEKVENGSE TGPLPPELQPLLEGEVKGGPEPTPLVQTFLRRQQRLQEIERRRNTPFHERFFRFLLRKLYVFRRSFLMTC ISLRNLILGRPSLEQLAQEVTYANLRPFEAVGELNPSNTDSSHSNPPESNPDPVHSEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000821 |
| ORF Size | 2274 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000821.7 |
| RefSeq Size | 7475 bp |
| RefSeq ORF | 2277 bp |
| Locus ID | 2677 |
| UniProt ID | P38435 |
| Domains | VKG_Carbox |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 87.6 kDa |
| Gene Summary | This gene encodes an integral membrane protein of the rough endoplasmic reticulum that carboxylates glutamate residues of vitamin K-dependent proteins to gamma carboxyl glutamate, a modification that is required for their activity. The vitamin K-dependent protein substrates have a propeptide that binds the enzyme, with carbon dioxide, dioxide, and reduced vitamin K acting as co-substrates. Vitamin K-dependent proteins affect a number of physiologic processes including blood coagulation, prevention of vascular calcification, and inflammation. Allelic variants of this gene have been associated with pseudoxanthoma elasticum-like disorder with associated multiple coagulation factor deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204003L3 | Lenti ORF clone of Human gamma-glutamyl carboxylase (GGCX), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC204003L4 | Lenti ORF clone of Human gamma-glutamyl carboxylase (GGCX), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG204003 | GGCX (tGFP-tagged) - Human gamma-glutamyl carboxylase (GGCX), transcript variant 1 |
CNY 7,152.00 |
|
| SC119634 | GGCX (untagged)-Human gamma-glutamyl carboxylase (GGCX), transcript variant 1 |
CNY 5,560.00 |
|
| SC320393 | GGCX (untagged)-Human gamma-glutamyl carboxylase (GGCX), transcript variant 1 |
CNY 5,560.00 |
