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SAMM50 (NM_015380) Human Tagged ORF Clone
CAT＃: RC204029
SAMM50 (Myc-DDK-tagged)-Human sorting and assembly machinery component 50 homolog (S. cerevisiae) (SAMM50)
ORF Plasmid: tGFP
"NM_015380" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-51; OMP85; SAM50; TOB55; TRG-3; YNL026W |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204029 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACTGTGCACGCCCGGAGTTTGGAGCCTCTTCCATCAAGTGGACCTGATTTTGGAGGATTAGGAG AAGAAGCTGAATTTGTTGAAGTTGAGCCTGAAGCTAAACAGGAAATTCTTGAAAACAAAGATGTGGTTGT TCAACATGTTCATTTTGATGGACTTGGAAGGACTAAAGATGATATCATCATTTGTGAAATTGGAGATGTT TTCAAGGCCAAAAACCTAATTGAGGTAATGCGGAAATCTCATGAAGCCCGTGAAAAATTGCTCCGTCTTG GAATTTTTAGACAAGTGGATGTTTTGATTGACACATGTCAAGGTGATGACGCACTTCCAAATGGGTTAGA CGTTACCTTTGAAGTAACTGAATTGAGGAGATTAACGGGCAGTTATAACACCATGGTTGGAAACAATGAA GGCAGTATGGTACTTGGCCTCAAGCTTCCTAATCTTCTTGGTCGTGCAGAAAAGGTGACCTTTCAGTTTT CCTATGGAACAAAAGAAACTTCGTATGGCCTGTCCTTCTTCAAACCACGGCCCGGAAACTTCGAAAGAAA TTTCTCTGTAAACTTATATAAAGTTACTGGACAGTTCCCTTGGAGCTCACTGCGGGAGACGGACAGAGGA ATGTCAGCTGAGTACAGTTTTCCCATATGGAAGACCAGCCACACTGTCAAGTGGGAAGGCGTATGGCGAG AACTGGGCTGCCTCTCAAGGACGGCGTCATTTGCTGTTCGAAAAGAAAGCGGACATTCACTGAAATCATC TCTTTCGCACGCCATGGTCATCGATTCTCGGAATTCTTCCATCTTACCAAGGAGAGGTGCTTTGCTGAAA GTTAACCAGGAACTGGCAGGCTACACTGGCGGGGATGTGAGCTTCATCAAAGAAGATTTTGAACTTCAGT TGAACAAGCAACTCATATTTGATTCAGTTTTTTCAGCGTCTTTCTGGGGCGGAATGTTGGTACCCATTGG TGATAAGCCGTCAAGCATTGCTGATAGGTTTTACCTCGGGGGACCCACAAGCGTCCGCGGATTCAGCATG CACAGCATCGGGCCACAGAGCGAAGGAGACTACCTAGGTGGAGAAGCGTACTGGGCCGGCGGCCTGCACC TCTACACCCCATTACCTTTCCGGCCAGGCCAGGGTGGCTTTGGAGAACTTTTCCGAACACACTTCTTTCT CAACGCAGGAAACCTCTGCAACCTCAACTATGGGGAGGGCCCCAAAGCTCATATTCGTAAGCTGGCTGAG TGCATCCGCTGGTCGTACGGGGCCGGGATTGTCCTCAGGCTTGGCAACATCGCTCGGTTGGAACTTAATT ACTGCGTCCCCATGGGAGTACAGACAGGCGACAGGATATGTGATGGCGTCCAGTTTGGAGCTGGGATAAG GTTCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204029 protein sequence
Red=Cloning site Green=Tags(s) MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDV FKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNE GSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPWSSLRETDRG MSAEYSFPIWKTSHTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRRGALLK VNQELAGYTGGDVSFIKEDFELQLNKQLIFDSVFSASFWGGMLVPIGDKPSSIADRFYLGGPTSVRGFSM HSIGPQSEGDYLGGEAYWAGGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKLAE CIRWSYGAGIVLRLGNIARLELNYCVPMGVQTGDRICDGVQFGAGIRFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015380 |
| ORF Size | 1407 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015380.3, NP_056195.2 |
| RefSeq Size | 1773 bp |
| RefSeq ORF | 1410 bp |
| Locus ID | 25813 |
| UniProt ID | Q9Y512 |
| Domains | Bac_surface_Ag |
| MW | 52 kDa |
| Gene Summary | This gene encodes a component of the Sorting and Assembly Machinery (SAM) of the mitochondrial outer membrane. The Sam complex functions in the assembly of beta-barrel proteins into the outer mitochondrial membrane.[provided by RefSeq, Jun 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204029L3 | Lenti ORF clone of Human sorting and assembly machinery component 50 homolog (S. cerevisiae) (SAMM50), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204029L4 | Lenti ORF clone of Human sorting and assembly machinery component 50 homolog (S. cerevisiae) (SAMM50), mGFP tagged |
CNY 5,890.00 |
|
| RG204029 | SAMM50 (tGFP-tagged) - Human sorting and assembly machinery component 50 homolog (S. cerevisiae) (SAMM50) |
CNY 5,256.00 |
|
| SC108289 | SAMM50 (untagged)-Human sorting and assembly machinery component 50 homolog (S. cerevisiae) (SAMM50) |
CNY 3,656.00 |
