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SH2D2A (NM_003975) Human Tagged ORF Clone
CAT＃: RC204162
- TrueORF®
SH2D2A (Myc-DDK-tagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 2
ORF Plasmid: tGFP
"NM_003975" in other vectors (5)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | F2771; SCAP; TSAD; VRAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204162 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCCCCTGGCCCAGATATGTCCCCAAGGGAGTCACGAAGCCCCCATCCCAACCTTCAGCACCT TCCAGATCACAGACATGACCCGCAGGAGCTGCCAGAACCTGGGCTACACTGCGGCATCTCCCCAGGCCCC GGAGGCTGCCTCCAGCACAGGGAATGCTGAGAGGGCAGAGGAGGTGCCTGGAGAAGGAAGCCTGTTCCTG CAGGCCGAGACCCGGGCTTGGTTCCAGAAGACCCAGGCCCACTGGCTCCTGCAGCACGGGGCAGCCCCTG CCTGGTTCCATGGCTTCATCACCCGGAGGGAGGCAGAGAGGCTGCTGGAGCCCAAGCCTCAGGGGTGCTA CTTGGTGCGGTTCAGCGAGAGCGCGGTGACCTTCGTGCTGACTTACAGGAGCCGGACTTGCTGCCGCCAC TTCCTGCTGGCCCAGCTCAGGGACGGGCGCCACGTGGTGCTGGGCGAGGACAGCGCCCACGCGCGGCTGC AGGACCTGCTGCTGCACTACACCGCGCACCCGCTCAGCCCCTACGGGGAGACGCTCACCGAGCCCCTCGC CCGACAGACTCCTGAGCCTGCAGGACTTTCCCTGAGGACCGAAGAATCAAACTTTGGAAGCAAAAGCCAG GACCCAAACCCCCAGTACAGCCCAATCATCAAACAGGGGCAAGCCCCAGTCCCGATGCAGAAAGAGGGGG CCGGGGAGAAGGAGCCCTCCCAGCTGCTCAGGCCCAAGCCTCCCATCCCCGCCAAACCTCAGCTGCCCCC AGAAGTCTACACAATCCCTGTTCCACGACACCGCCCGGCCCCACGCCCCAAGCCCTCCAATCCTATCTAC AATGAGCCTGATGAACCCATAGCTTTCTATGCCATGGGCCGGGGCAGCCCTGGGGAAGCCCCCAGCAACA TCTATGTGGAAGTGGAAGATGAGGGCCTACCCGCCACCCTTGGGCACCCTGTCCTACGGAAGAGCTGGTC CAGGCCTGTCCCAGGAGGCCAGAATACAGGTGGCTCCCAGCTGCATTCTGAGAACTCTGTGATTGGGCAA GGCCCTCCCCTGCCCCACCAGCCCCCACCCGCCTGGAGACACACCCTCCCCCACAATCTTTCTAGACAGG TGCTTCAGGACAGAGGACAGGCATGGCTTCCCCTTGGGCCTCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204162 protein sequence
Red=Cloning site Green=Tags(s) MEFPLAQICPQGSHEAPIPTFSTFQITDMTRRSCQNLGYTAASPQAPEAASSTGNAERAEEVPGEGSLFL QAETRAWFQKTQAHWLLQHGAAPAWFHGFITRREAERLLEPKPQGCYLVRFSESAVTFVLTYRSRTCCRH FLLAQLRDGRHVVLGEDSAHARLQDLLLHYTAHPLSPYGETLTEPLARQTPEPAGLSLRTEESNFGSKSQ DPNPQYSPIIKQGQAPVPMQKEGAGEKEPSQLLRPKPPIPAKPQLPPEVYTIPVPRHRPAPRPKPSNPIY NEPDEPIAFYAMGRGSPGEAPSNIYVEVEDEGLPATLGHPVLRKSWSRPVPGGQNTGGSQLHSENSVIGQ GPPLPHQPPPAWRHTLPHNLSRQVLQDRGQAWLPLGPPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003975 |
| ORF Size | 1167 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003975.2, NP_003966.1 |
| RefSeq Size | 1661 bp |
| RefSeq ORF | 1170 bp |
| Locus ID | 9047 |
| UniProt ID | Q9NP31 |
| Domains | SH2 |
| Protein Pathways | VEGF signaling pathway |
| MW | 42.9 kDa |
| Gene Summary | This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204162L3 | Lenti ORF clone of Human SH2 domain containing 2A (SH2D2A), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204162L4 | Lenti ORF clone of Human SH2 domain containing 2A (SH2D2A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG204162 | SH2D2A (tGFP-tagged) - Human SH2 domain containing 2A (SH2D2A), transcript variant 2 |
CNY 5,256.00 |
|
| SC117636 | SH2D2A (untagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 2 |
CNY 3,656.00 |
|
| SC320782 | SH2D2A (untagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 2 |
CNY 3,656.00 |
