HPS3 (NM_032383) Human Tagged ORF Clone
CAT#: RC204569
HPS3 (Myc-DDK-tagged)-Human Hermansky-Pudlak syndrome 3 (HPS3)
ORF Plasmid: tGFP
"NM_032383" in other vectors (4)
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CNY 6,992.00
CNY 7,600.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BLOC2S1; SUTAL |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204569 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGCTGTACAACCTGCACCCGTTCGGGTCGCAGCAGGTGGTGCCCTGCAAGCTGGAGCCGGACC GGTTCTGTGGCGGGGGGCGTGACGCGCTTTTCGTGGCGGCGGGCTGCAAGGTGGAGGCGTTCGCGGTGGC CGGCCAGGAGCTGTGCCAGCCGCGGTGCGCCTTCTCCACGCTGGGCCGGGTGTTGCGCCTGGCCTACAGC GAGGCTGGAGATTATTTGGTAGCAATTGAAGAGAAAAACAAAGCTACATTTCTACGTGCTTATGTGAACT GGAGAAATAAAAGGACTGAAAACTCTCGTGTGTGTATCCGAATGATTGGGCATAATGTGGAGGGACCATT CAGCAAAGCCTTCAGAGACCAGATGTACATTATTGAAATGCCGCTTTCGGAGGCCCCCTTGTGCATTTCC TGTTGCCCTGTGAAAGGAGACCTTCTCGTTGGCTGCACAAATAAATTAGTCTTATTTAGTTTGAAGTACC AGATCATTAATGAGGAATTCTCACTATTGGACTTTGAACGTTCTTTAATTATACACATAGATAATATCAC TCCTGTTGAGGTTTCTTTTTGTGTTGGATATGTTGCTGTCATGTCAGACTTAGAAGTCTTAATCGTAAAA CTGGAGTCAGGCCCTAAAAATGGAGAGAGAGTTCACCACCATCCACATAAGACCAACAATCGAATAAGAC GGACAGAAGAAGGCATCAGTAATGAAATTTCACAGCTTGAGTCAGATGATTTTGTCATCTGCCAGAAGCC CCTGGAACTTCTTGGTGAAAAAAGTGAACAGTCTGGATTATCTGTTACACTGGAGTCTACGGGATTAGCT GATGAAAAAAGAAAATATTCCCACTTTCAGCACCTGCTCTATAGACGTTTTGCTCCTGATATTTCGTCCT ATGTCTTGTCTGATGACATCAAGCTACATTCCCTCCAGCTGCTACCCATTTACCAGACCGGTTCTCTTAC ATCTGATGGAAAAAATTTGTCTCAGGAAAAAGAATTGCTGAGTCTCTTTTGCTTTTTCTCCTTACCTCAT GTGGGCTATCTCTACATGGTTGTCAAATCTGTTGAATTGATGTCAGTCTACCAGTATCCTGAAAAGTCTC AGCAGGCAGTACTCACGCCACAATTTTTGCACGTCATTACAAGTAACAACCTGCAGTGTTTCACTGTGCG GTGCAGTGCGGCGGCAGCTCGTGAGGAGGACCCGTACATGGACACCACCCTGAAGGCTTGCCCACCTGTC AGTATGGATGTCTGTGCTTTAAGAATACAGCTTTTCATAGGCTTGAAAGCCATCTGTCACTTTAAAAACC ACATCATACTTTTGACTAAAGCAGAACCTGAAGCCATTCCAGAGAGAAGACAGTCACCCAAGAGGCTTCT TTCGAGAAAAGATACCAGTGTTAAAATCAAAATACCTCCTGTAGCTGAGGCTGGGTGGAATTTGTATATT GTGAATACGATCTCACCAGTGCAACTGTACAAAGAGATGGTAGACTATAGCAATACCTATAAGACTGTCA AAACCCAGAGCTGCATTCACCTTCTCAGTGAGGCTCATCTGTTAGTGCGAGCTGCCCTGATGGATGCCAG TCAGCTGGAACCTGGAGAGAAGGCAGAGCTTTTGGAAGCATTTAAGGAAAGCTGTGGGCACCTTGGGGAC TGTTACAGCAGGCTTGACTCCCAGCATTCTCATCTCACCTTGCCATACTATAAGATGTCTGGTTTGTCTA TGGCTGAAGTTCTGGCCCGCACGGACTGGACAGTAGAGGATGGATTACAGAAATACGAGAGAGGATTAAT CTTTTACATTAATCATTCACTTTATGAAAACCTGGATGAAGAATTAAATGAAGAATTAGCAGCAAAAGTG GTTCAGATGTTTTATGTGGCTGAGCCAAAGCAAGTGCCCCATATTCTCTGTAGTCCTTCTATGAAGAATA TTAATCCTTTAACTGCCATGAGCTATCTAAGGAAGCTGGATACTTCTGGGTTTTCATCGATCTTAGTGAC ATTGACCAAGGCAGCAGTGGCTCTGAAAATGGGAGATCTTGACATGCACAGAAATGAAATGAAAAGCCAT TCAGAGATGAAGTTGGTATGTGGCTTCATTCTGGAACCTCGGCTGTTGATTCAACAGAGAAAGGGACAGA TTGTTCCAACCGAGCTTGCACTTCACTTGAAGGAAACTCAGCCTGGATTGCTTGTGGCTTCAGTTCTGGG CTTGCAGAAGAACAACAAAATTGGAATTGAAGAAGCAGATTCCTTTTTTAAGGTGCTTTGTGCTAAGGAT GAAGATACAATTCCTCAGCTCTTGGTAGACTTTTGGGAAGCTCAGCTAGTGGCATGTCTCCCAGATGTGG TACTTCAGGAACTCTTTTTCAAACTCACATCACAGTACATCTGGAGATTGTCTAAGAGGCAGCCTCCTGA CACCACACCATTGCGAACATCGGAGGATCTGATAAATGCCTGTAGTCATTATGGCTTAATTTATCCATGG GTTCACGTCGTAATATCATCTGATTCTTTAGCTGATAAAAATTATACAGAAGATCTTTCAAAATTACAGT CTCTTATATGTGGTCCTTCATTTGACATAGCTTCCATTATTCCGTTCTTGGAGCCACTTTCAGAAGACAC TATTGCCGGCCTCAGTGTCCATGTTCTGTGTCGTACACGCTTGAAAGAGTATGAACAGTGCATAGACATA CTGTTAGAGAGATGCCCGGAGGCAGTCATTCCATATGCTAATCATGAACTGAAAGAAGAGAACCGGACTC TGTGGTGGAAAAAACTGTTGCCTGAACTTTGTCAGAGAATAAAATGTGGTGGAGAGAAGTATCAACTCTA CCTGTCATCATTAAAAGAAACATTGTCAATTGTTGCTGTGGAACTAGAACTGAAGGATTTCATGAATGTT CTCCCAGAAGATGGTACTGCAACATTTTTCTTGCCATATCTTCTCTATTGCAGTCGAAAGAAACCATTGA CT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204569 protein sequence
Red=Cloning site Green=Tags(s) MVQLYNLHPFGSQQVVPCKLEPDRFCGGGRDALFVAAGCKVEAFAVAGQELCQPRCAFSTLGRVLRLAYS EAGDYLVAIEEKNKATFLRAYVNWRNKRTENSRVCIRMIGHNVEGPFSKAFRDQMYIIEMPLSEAPLCIS CCPVKGDLLVGCTNKLVLFSLKYQIINEEFSLLDFERSLIIHIDNITPVEVSFCVGYVAVMSDLEVLIVK LESGPKNGERVHHHPHKTNNRIRRTEEGISNEISQLESDDFVICQKPLELLGEKSEQSGLSVTLESTGLA DEKRKYSHFQHLLYRRFAPDISSYVLSDDIKLHSLQLLPIYQTGSLTSDGKNLSQEKELLSLFCFFSLPH VGYLYMVVKSVELMSVYQYPEKSQQAVLTPQFLHVITSNNLQCFTVRCSAAAAREEDPYMDTTLKACPPV SMDVCALRIQLFIGLKAICHFKNHIILLTKAEPEAIPERRQSPKRLLSRKDTSVKIKIPPVAEAGWNLYI VNTISPVQLYKEMVDYSNTYKTVKTQSCIHLLSEAHLLVRAALMDASQLEPGEKAELLEAFKESCGHLGD CYSRLDSQHSHLTLPYYKMSGLSMAEVLARTDWTVEDGLQKYERGLIFYINHSLYENLDEELNEELAAKV VQMFYVAEPKQVPHILCSPSMKNINPLTAMSYLRKLDTSGFSSILVTLTKAAVALKMGDLDMHRNEMKSH SEMKLVCGFILEPRLLIQQRKGQIVPTELALHLKETQPGLLVASVLGLQKNNKIGIEEADSFFKVLCAKD EDTIPQLLVDFWEAQLVACLPDVVLQELFFKLTSQYIWRLSKRQPPDTTPLRTSEDLINACSHYGLIYPW VHVVISSDSLADKNYTEDLSKLQSLICGPSFDIASIIPFLEPLSEDTIAGLSVHVLCRTRLKEYEQCIDI LLERCPEAVIPYANHELKEENRTLWWKKLLPELCQRIKCGGEKYQLYLSSLKETLSIVAVELELKDFMNV LPEDGTATFFLPYLLYCSRKKPLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_032383 |
ORF Size | 3012 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_032383.5 |
RefSeq Size | 4451 bp |
RefSeq ORF | 3015 bp |
Locus ID | 84343 |
UniProt ID | Q969F9 |
MW | 113.7 kDa |
Gene Summary | This gene encodes a protein containing a potential clathrin-binding motif, consensus dileucine signals, and tyrosine-based sorting signals for targeting to vesicles of lysosomal lineage. The encoded protein may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 3. [provided by RefSeq, Apr 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204569L3 | Lenti ORF clone of Human Hermansky-Pudlak syndrome 3 (HPS3), Myc-DDK-tagged |
CNY 10,450.00 |
|
RC204569L4 | Lenti ORF clone of Human Hermansky-Pudlak syndrome 3 (HPS3), mGFP tagged |
CNY 10,450.00 |
|
RG204569 | HPS3 (tGFP-tagged) - Human Hermansky-Pudlak syndrome 3 (HPS3) |
CNY 8,592.00 |
|
SC127283 | HPS3 (untagged)-Human Hermansky-Pudlak syndrome 3 (HPS3) |
CNY 7,000.00 |