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BAAT1 (BRAT1) (NM_152743) Human Tagged ORF Clone
CAT＃: RC204822
BRAT1 (Myc-DDK-tagged)-Human BRCA1-associated ATM activator 1 (BRAT1)
ORF Plasmid: tGFP
"NM_152743" in other vectors (7)
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CNY 6,016.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BAAT1; C7orf27; NEDCAS; RMFSL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204822 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCCAGAATGCGCCCAGCTGCTCCCGGCTCTCTGTGCTGTTCTGGTAGATCCCGGGCAGCCGGTGG CAGATGACACCTGTTTGGAGAAGCTCCTGGACTGGTTTAAAACGGTCACTGAAGGAGAGTCCAGTGTCGT GCTGCTGCAGGAGCACCCCTGCCTGGTGGAGCTGCTGTCCCATGTGCTGAAAGTCCAGGACCTGAGTTCT GGGGTCCTCTCCTTCTCACTGCGCCTGGCAGGAACCTTCGCAGCCCAGGAAAACTGCTTCCAGTATCTTC AGCAGGGGGAGTTACTACCAGGGCTCTTTGGGGAGCCAGGACCCCTCGGCCGAGCAACCTGGGCCGTCCC CACCGTGCGCAGCGGCTGGATCCAGGGCCTGCGCTCCCTGGCACAGCACCCCAGCGCCCTGCGCTTCCTG GCCGACCATGGTGCGGTCGACACCATCTTCTCCCTGCAGGGAGACTCCAGCCTGTTTGTGGCCTCGGCGG CCAGTCAGCTCCTGGTGCACGTCCTGGCTTTGTCCATGCGAGGTGGAGCCGAGGGGCAGCCCTGCCTGCC GGGGGGTGACTGGCCCGCGTGTGCCCAGAAGATCATGGATCACGTTGAAGAGTCCTTGTGCTCCGCGGCC ACCCCCAAGGTCACTCAGGCCCTGAACGTCCTGACCACGACCTTCGGGCGCTGCCAGAGCCCCTGGACGG AAGCCCTGTGGGTGCGGCTGAGTCCCCGCGTGGCCTGTCTGCTGGAGAGAGACCCCATCCCCGCCGCACA CTCGTTCGTGGACCTGCTTCTCTGTGTGGCTCGTTCTCCCGTGTTCAGTTCTTCCGACGGCAGCCTGTGG GAGACAGTGGCGCGGGCTCTGAGCTGCCTGGGTCCCACCCACATGGGACCCCTGGCTTTGGGGATCCTGA AGCTCGAGCACTGTCCACAGGCACTGAGGACCCAGGCCTTCCAGGTCCTTCTCCAGCCCCTGGCCTGTGT CCTGAAGGCCACGGTTCAGGCCCCCGGACCCCCAGGCTTGCTGGACGGGACGGCAGACGATGCCACGACG GTGGACACACTCCTGGCCTCCAAGTCGTCCTGCGCCGGCCTCCTGTGCCGCACCCTGGCTCACCTGGAGG AGCTGCAGCCGCTGCCCCAGCGCCCTTCACCGTGGCCCCAGGCGTCTCTACTGGGGGCTACAGTGACTGT CCTGCGGCTCTGTGACGGCTCGGCTGCCCCTGCCTCCAGTGTGGGGGGCCACCTCTGTGGGACCCTGGCG GGCTGCGTCCGGGTCCAGCGAGCAGCCCTCGACTTCCTGGGGACGCTGTCACAGGGGACAGGCCCCCAGG AGCTGGTGACGCAGGCGCTTGCTGTCCTCCTGGAGTGCCTCGAGAGCCCCGGCTCCAGCCCCACGGTTCT GAAGAAGGCCTTCCAGGCCACGCTCAGGTGGCTCCTGAGCTCACCCAAGACCCCCGGCTGCTCTGATCTC GGCCCCCTCATCCCGCAGTTCCTCAGAGAGCTGTTCCCTGTGCTGCAGAAACGCCTGTGCCACCCCTGCT GGGAGGTGAGGGACTCCGCCCTCGAGTTCCTGACCCAGCTGAGCAGGCACTGGGGAGGACAGGCTGACTT CAGATGCGCACTCTTGGCTTCAGAGGTGCCTCAGCTGGCCCTGCAGCTCCTCCAGGACCCTGAGAGTTAT GTCCGAGCGAGTGCAGTGACCGCCATGGGGCAGCTGTCCAGCCAGGGCCTGCACGCCCCCACCAGCCCTG AGCATGCAGAGGCCCGGCAGAGCCTGTTCCTGGAGCTCCTGCACATCCTCTCCGTAGACTCGGAGGGCTT CCCACGGCGGGCGGTCATGCAAGTCTTCACTGAGTGGCTGCGGGACGGCCACGCCGACGCGGCCCAGGAC ACGGAGCAGTTCGTGGCCACTGTGCTGCAGGCGGCGAGCCGAGACCTGGACTGGGAGGTCCGCGCCCAGG GCCTGGAGCTGGCCCTCGTGTTCCTGGGCCAGACTTTGGGGCCGCCGCGTACCCACTGCCCCTATGCCGT GGCCCTACCCGAGGTGGCCCCAGCCCAGCCACTCACCGAGGCACTGAGGGCTCTCTGCCACGTGGGGCTC TTTGACTTCGCCTTTTGTGCCTTGTTTGACTGCGACCGCCCTGTGGCGCAGAAGTCTTGTGACCTCCTTC TCTTCCTGAGGGACAAGATTGCTTCCTACAGCAGCCTGCGGGAGGCCAGGGGCAGCCCCAACACTGCCTC CGCAGAGGCCACCCTGCCGAGGTGGCGGGCGGGTGAGCAGGCCCAGCCCCCAGGGGACCAGGAGCCTGAG GCTGTGCTGGCCATGCTCAGGTCCCTAGACCTGGAGGGCCTGCGGAGCACGCTGGCCGAGAGCAGCGACC ACGTGGAAAAGAGTCCCCAGTCCCTCCTGCAGGACATGCTGGCCACGGGAGGCTTCCTGCAGGGGGACGA GGCCGACTGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204822 protein sequence
Red=Cloning site Green=Tags(s) MDPECAQLLPALCAVLVDPGQPVADDTCLEKLLDWFKTVTEGESSVVLLQEHPCLVELLSHVLKVQDLSS GVLSFSLRLAGTFAAQENCFQYLQQGELLPGLFGEPGPLGRATWAVPTVRSGWIQGLRSLAQHPSALRFL ADHGAVDTIFSLQGDSSLFVASAASQLLVHVLALSMRGGAEGQPCLPGGDWPACAQKIMDHVEESLCSAA TPKVTQALNVLTTTFGRCQSPWTEALWVRLSPRVACLLERDPIPAAHSFVDLLLCVARSPVFSSSDGSLW ETVARALSCLGPTHMGPLALGILKLEHCPQALRTQAFQVLLQPLACVLKATVQAPGPPGLLDGTADDATT VDTLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVGGHLCGTLA GCVRVQRAALDFLGTLSQGTGPQELVTQALAVLLECLESPGSSPTVLKKAFQATLRWLLSSPKTPGCSDL GPLIPQFLRELFPVLQKRLCHPCWEVRDSALEFLTQLSRHWGGQADFRCALLASEVPQLALQLLQDPESY VRASAVTAMGQLSSQGLHAPTSPEHAEARQSLFLELLHILSVDSEGFPRRAVMQVFTEWLRDGHADAAQD TEQFVATVLQAASRDLDWEVRAQGLELALVFLGQTLGPPRTHCPYAVALPEVAPAQPLTEALRALCHVGL FDFAFCALFDCDRPVAQKSCDLLLFLRDKIASYSSLREARGSPNTASAEATLPRWRAGEQAQPPGDQEPE AVLAMLRSLDLEGLRSTLAESSDHVEKSPQSLLQDMLATGGFLQGDEADCY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152743 |
| ORF Size | 2463 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152743.2, NP_689956.1 |
| RefSeq Size | 3013 bp |
| RefSeq ORF | 2466 bp |
| Locus ID | 221927 |
| UniProt ID | Q6PJG6 |
| MW | 88 kDa |
| Gene Summary | The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM. [provided by RefSeq, Mar 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204822L1 | Lenti ORF clone of Human BRCA1-associated ATM activator 1 (BRAT1), Myc-DDK-tagged |
CNY 8,416.00 |
|
| RC204822L2 | Lenti ORF clone of Human BRCA1-associated ATM activator 1 (BRAT1), mGFP tagged |
CNY 5,890.00 |
|
| RC204822L3 | Lenti ORF clone of Human BRCA1-associated ATM activator 1 (BRAT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204822L4 | Lenti ORF clone of Human BRCA1-associated ATM activator 1 (BRAT1), mGFP tagged |
CNY 5,890.00 |
|
| RG204822 | BRAT1 (tGFP-tagged) - Human BRCA1-associated ATM activator 1 (BRAT1) |
CNY 4,370.00 |
|
| SC126644 | BRAT1 (untagged)-Human BRCA1-associated ATM activator 1 (BRAT1) |
CNY 6,024.00 |
|
| SC320446 | BRAT1 (untagged)-Human BRCA1-associated ATM activator 1 (BRAT1) |
CNY 6,024.00 |
