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PC4 (SUB1) (NM_006713) Human Tagged ORF Clone
CAT＃: RC204999
SUB1 (Myc-DDK-tagged)-Human SUB1 homolog (S. cerevisiae) (SUB1)
ORF Plasmid: tGFP
"NM_006713" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
| Cited in 3 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p14; P15; PC4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204999 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTAAATCAAAGGAACTTGTTTCTTCAAGCTCTTCTGGCAGTGATTCTGACAGTGAGGTTGACAAAA AGTTAAAGAGGAAAAAGCAAGTTGCTCCAGAAAAACCTGTAAAGAAACAAAAGACAGGTGAGACTTCGAG AGCCCTGTCATCTTCTAAACAGAGCAGCAGCAGCAGAGATGATAACATGTTTCAGATTGGGAAAATGAGG TACGTTAGTGTTCGCGATTTTAAAGGCAAAGTGCTAATTGATATTAGAGAATATTGGATGGATCCTGAAG GTGAAATGAAACCAGGAAGAAAAGGTATTTCTTTAAATCCAGAACAATGGAGCCAGCTGAAGGAACAGAT TTCTGACATTGATGATGCAGTAAGAAAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204999 protein sequence
Red=Cloning site Green=Tags(s) MPKSKELVSSSSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKTGETSRALSSSKQSSSSRDDNMFQIGKMR YVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQISDIDDAVRKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006713 |
| ORF Size | 381 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006713.4 |
| RefSeq Size | 3522 bp |
| RefSeq ORF | 384 bp |
| Locus ID | 10923 |
| UniProt ID | P53999 |
| Domains | PC4 |
| Protein Families | Transcription Factors |
| MW | 14.4 kDa |
| Gene Summary | General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).[UniProtKB/Swiss-Prot Function] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Interaction of ASOs with PC4 Is Highly Influenced by the Cellular Environment and ASO Chemistry
,Vickers, TA;Migawa, MT;Seth, PP;Crooke, ST;,
J. Am. Chem. Soc.
,PubMed ID 32374993
[PC4]
|
|
Origins of the increased affinity of phosphorothioate-modified therapeutic nucleic acids for proteins
,Hyjek-Składanowska, M;Vickers, T;Napiórkowska, A;Anderson, B;Tanowitz, M;Crooke, ST;Liang, XH;Seth, PP;Nowotny, M;,
J. Am. Chem. Soc.
,PubMed ID 32202774
[PC4]
|
|
MicroRNA-101 regulated transcriptional modulator SUB1 plays a role in prostate cancer
,Chakravarthi, BV;Goswami, MT;Pathi, SS;Robinson, AD;Cieslik, M;Chandrashekar, DS;Agarwal, S;Siddiqui, J;Daignault, S;Carskadon, SL;Jing, X;Chinnaiyan, AM;Kunju, LP;Palanisamy, N;Varambally, S;,
Oncogene
,PubMed ID 27270442
[PC4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204999L1 | Lenti ORF clone of Human SUB1 homolog (S. cerevisiae) (SUB1), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC204999L2 | Lenti ORF clone of Human SUB1 homolog (S. cerevisiae) (SUB1), mGFP tagged |
CNY 5,890.00 |
|
| RC204999L3 | Lenti ORF clone of Human SUB1 homolog (S. cerevisiae) (SUB1), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC204999L4 | Lenti ORF clone of Human SUB1 homolog (S. cerevisiae) (SUB1), mGFP tagged |
CNY 3,600.00 |
|
| RG204999 | SUB1 (tGFP-tagged) - Human SUB1 homolog (S. cerevisiae) (SUB1) |
CNY 2,800.00 |
|
| SC115927 | SUB1 (untagged)-Human SUB1 homolog (S. cerevisiae) (SUB1) |
CNY 1,200.00 |
