PRMT8 (NM_019854) Human Tagged ORF Clone
CAT#: RC205188
PRMT8 (Myc-DDK-tagged)-Human protein arginine methyltransferase 8 (PRMT8)
ORF Plasmid: tGFP
"NM_019854" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HRMT1L3; HRMT1L4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205188 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATGAAACACTCCTCCCGCTGCCTGCTCCTGAGGAGGAAAATGGCGGAGAACGCGGCCGAGAGCA CCGAGGTGAACAGCCCCCCCTCCCAGCCCCCCCAGCCCGTCGTCCCTGCTAAGCCCGTGCAATGCGTCCA TCATGTGTCCACTCAACCCAGCTGCCCAGGACGGGGCAAGATGTCCAAGCTGCTGAACCCAGAGGAGATG ACCTCGAGAGATTATTACTTCGACTCCTATGCCCACTTTGGGATCCACGAGGAAATGCTGAAGGATGAGG TGCGGACTCTCACTTACCGGAACTCCATGTACCACAACAAGCACGTGTTCAAGGACAAAGTGGTACTGGA TGTGGGGAGTGGTACTGGGATCCTTTCCATGTTCGCTGCCAAGGCAGGGGCCAAGAAGGTGTTTGGGATC GAATGCTCCAGTATTTCTGACTACTCACAGAAGATCATTAAGGCCAACCACTTGGACAACATCATCACCA TATTTAAGGGTAAAGTGGAAGAGGTGGAGCTGCCTGTGGAGAAGGTGGACATCATCATCAGCGAGTGGAT GGGCTACTGTCTGTTCTATGAGTCCATGCTCAACACGGTGATCTTTGCCAGGGACAAGTGGCTGAAACCT GGAGGGCTTATGTTTCCAGACCGGGCAGCTTTGTACGTGGTAGCGATTGAAGACAGACAGTACAAGGACT TCAAAATCCACTGGTGGGAGAATGTCTATGGCTTTGACATGACCTGCATCAGGGACGTGGCCATGAAGGA GCCTCTAGTGGACATCGTGGATCCAAAGCAAGTGGTGACCAATGCCTGTTTGATAAAGGAGGTGGACATT TACACAGTGAAGACGGAAGAGCTATCGTTCACATCTGCATTCTGCCTGCAGATACAGCGCAACGACTACG TCCACGCCCTGGTCACCTATTTTAATATTGAATTTACCAAGTGCCACAAGAAAATGGGGTTTTCCACAGC CCCTGATGCTCCCTACACCCACTGGAAGCAGACCGTCTTCTACTTGGAAGATTACCTCACTGTCCGGAGG GGGGAGGAAATCTACGGGACCATATCCATGAAGCCAAATGCCAAAAATGTGCGAGACCTCGATTTCACAG TAGACTTGGATTTTAAGGGACAGCTGTGTGAAACATCTGTATCTAATGACTACAAAATGCGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205188 protein sequence
Red=Cloning site Green=Tags(s) MGMKHSSRCLLLRRKMAENAAESTEVNSPPSQPPQPVVPAKPVQCVHHVSTQPSCPGRGKMSKLLNPEEM TSRDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMYHNKHVFKDKVVLDVGSGTGILSMFAAKAGAKKVFGI ECSSISDYSQKIIKANHLDNIITIFKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVIFARDKWLKP GGLMFPDRAALYVVAIEDRQYKDFKIHWWENVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIKEVDI YTVKTEELSFTSAFCLQIQRNDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTHWKQTVFYLEDYLTVRR GEEIYGTISMKPNAKNVRDLDFTVDLDFKGQLCETSVSNDYKMR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019854 |
ORF Size | 1182 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019854.5 |
RefSeq Size | 2417 bp |
RefSeq ORF | 1185 bp |
Locus ID | 56341 |
UniProt ID | Q9NR22 |
Protein Families | Druggable Genome |
MW | 45.3 kDa |
Gene Summary | Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Protein arginine methyltransferase 8 gene enhances the colon cancer stem cell (CSC) function by upregulating the pluripotency transcription factor
,Lin, H;Wang, B;Yu, J;Wang, J;Li, Q;Cao, B;,
J Cancer
,PubMed ID 29721049
[PRMT8]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205188L1 | Lenti ORF clone of Human protein arginine methyltransferase 8 (PRMT8), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC205188L2 | Lenti ORF clone of Human protein arginine methyltransferase 8 (PRMT8), mGFP tagged |
CNY 5,890.00 |
|
RC205188L3 | Lenti ORF clone of Human protein arginine methyltransferase 8 (PRMT8), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205188L4 | Lenti ORF clone of Human protein arginine methyltransferase 8 (PRMT8), mGFP tagged |
CNY 5,890.00 |
|
RG205188 | PRMT8 (tGFP-tagged) - Human protein arginine methyltransferase 8 (PRMT8) |
CNY 5,256.00 |
|
SC108108 | PRMT8 (untagged)-Human protein arginine methyltransferase 8 (PRMT8) |
CNY 3,656.00 |