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CBF (CEBPZ) (NM_005760) Human Tagged ORF Clone
CAT＃: RC205283
- TrueORF®
CEBPZ (Myc-DDK-tagged)-Human CCAAT/enhancer binding protein (C/EBP), zeta (CEBPZ)
ORF Plasmid: tGFP
"NM_005760" in other vectors (4)
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CNY 7,336.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CBF; CBF2; HSP-CBF; NOC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205283 representing NM_005760
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCAGTCAAGGAGCCTTTGGAGTTCCATGCCAAGCGGCCTTGGCGCCCCGAGGAGGCAGTAGAAG ATCCGGACGAGGAGGATGAGGATAATACTAGTGAAGCCGAGAATGGGTTCTCCCTGGAGGAAGTGTTACG GCTCGGAGGCACCAAGCAAGATTACCTTATGCTGGCTACTTTGGATGAGAATGAGGAAGTGATAGATGGA GGCAAAAAAGGAGCAATCGATGACCTTCAGCAAGGTGAATTGGAAGCATTTATTCAAAATCTTAATTTGG CGAAGTATACAAAAGCTTCCTTAGTTGAAGAAGATGAACCAGCTGAAAAAGAAAATTCCAGCAAAAAAGA AGTAAAAATACCTAAAATAAATAATAAAAATACAGCAGAAAGTCAAAGGACATCAGTTAATAAGGTGAAA AATAAGAATAGGCCAGAACCACATTCTGATGAGAATGGCAGTACCACACCGAAAGTAAAGAAAGATAAAC AGAACATCTTTGAATTTTTTGAGAGACAGACTTTGTTACTTAGGCCTGGAGGCAAATGGTATGATCTGGA GTACAGCAATGAATATTCTTTGAAACCCCAGCCTCAGGATGTTGTATCTAAGTACAAAACCCTTGCTCAG AAGCTGTATCAGCATGAAATCAACTTATTCAAAAGTAAGACGAATAGTCAAAAGGGAGCCTCTTCTACCT GGATGAAGGCAATTGTGTCATCGGGGACACTAGGTGACAGGATGGCAGCCATGATTCTTCTTATTCAGGA TGATGCCGTTCACACACTTCAGTTTGTAGAAACTCTTGTGAACCTTGTTAAAAAGAAGGGCAGCAAACAG CAGTGCCTTATGGCCTTGGATACTTTCAAAGAGTTGCTTATCACAGACCTTTTGCCAGACAATCGGAAGC TGAGGATTTTCAGCCAGCGTCCTTTTGACAAACTGGAACAGTTGTCCAGTGGCAACAAGGACTCAAGAGA TAGAAGACTGATATTATGGTATTTTGAACACCAGCTGAAACACTTAGTGGCTGAATTTGTGCAGGTCTTA GAAACTTTAAGTCATGATACATTAGTAACCACTAAAACTCGAGCCCTTACCGTGGCTCATGAGCTGCTTT GTAACAAGCCTGAGGAAGAAAAGGCTCTTCTTGTGCAAGTGGTAAATAAACTGGGAGATCCTCAGAACAG AATTGCCACAAAAGCATCCCATCTGTTAGAGACATTACTTTGTAAACATCCCAATATGAAAGGAGTTGTG TCTGGTGAAGTAGAAAGGCTACTCTTCCGCTCAAATATCAGCTCCAAAGCTCAATATTATGCAATTTGCT TTTTAAATCAAATGGCTCTGTCCCATGAAGAAAGTGAATTGGCTAACAAATTAATAACTGTTTACTTTTG CTTTTTTCGGACTTGTGTCAAAAAAAAAGATGTTGAATCAAAAATGCTTAGCGCCCTTTTAACAGGTGTG AATAGGGCATACCCTTATTCCCAGACTGGTGATGACAAAGTAAGGGAGCAGATTGACACACTGTTTAAAG TGTTGCATATTGTGAATTTTAATACCAGTGTCCAGGCTTTAATGTTGCTTTTCCAAGTAATGAATTCTCA GCAGACAATATCGGATCGATATTACACAGCATTATACAGGAAGATGTTGGATCCAGGGTTGATGACGTGT TCCAAGCAAGCTATGTTTCTTAACCTTGTCTACAAATCTCTGAAAGCTGACATTGTGTTGCGCCGGGTGA AGGCTTTTGTGAAGAGGTTACTTCAAGTTACTTGTCAACAGATGCCACCATTTATATGTGGAGCTTTATA TCTTGTGTCTGAGATCCTTAAAGCAAAACCAGGTTTAAGAAGCCAACTAGATGATCATCCGGAGTCTGAT GATGAAGAAAATTTTATTGATGCAAATGATGATGAAGACATGGAAAAATTCACTGATGCAGACAAAGAAA CAGAGATAGTGAAAAAACTTGAGACAGAGGAAACAGTTCCTGAAACTGATGTAGAAACCAAAAAACCAGA GGTTGCTTCCTGGGTGCACTTTGATAATTTGAAAGGTGGGAAACAGTTAAATAAATACGATCCATTCAGT AGAAACCCTCTGTTCTGTGGAGCTGAAAATACAAGTCTTTGGGAACTCAAAAAGTTATCTGTGCATTTTC ATCCCTCCGTGGCCCTTTTTGCAAAGACCATCCTTCAGGGAAACTATATTCAGTATTCAGGGGACCCACT GCAGGATTTCACTCTAATGAGATTTTTGGATCGATTTGTATACCGAAATCCAAAGCCCCATAAAGGCAAA GAAAACACAGATAGTGTTGTGATGCAGCCGAAAAGAAAACATTTTATTAAGGATATTCGTCATCTTCCTG TGAACAGTAAGGAGTTCCTTGCAAAAGAAGAAAGCCAAATACCAGTGGATGAAGTGTTTTTCCACAGGTA TTATAAAAAAGTTGCTGTTAAAGAGAAACAAAAACGGGATGCAGATGAAGAAAGTATAGAAGACGTGGAT GATGAAGAATTTGAAGAGCTGATTGACACATTTGAAGATGATAACTGTTTCAGCTCTGGAAAGGATGATA TGGATTTTGCTGGAAACGTGAAAAAGAGAACAAAAGGAGCTAAGGATAACACATTAGATGAAGATTCAGA AGGTAGTGATGATGAACTTGGTAACCTGGATGACGATGAAGTTTCTTTAGGAAGTATGGATGATGAAGAA TTTGCTGAAGTTGATGAAGATGGAGGAACATTCATGGATGTGTTAGATGATGAAAGTGAGAGCGTTCCAG AACTTGAAGTCCACTCCAAAGTCAGTACTAAGAAAAGCAAGAGAAAAGGTACAGATGATTTTGACTTTGC TGGCTCATTTCAAGGGCCAAGAAAAAAGAAAAGAAACTTAAATGATTCCAGCCTATTTGTATCTGCTGAA GAGTTTGGCCATCTATTGGATGAAAATATGGGATCCAAGTTTGATAACATTGGCATGAATGCCATGGCTA ACAAAGATAATGCAAGTCTCAAACAGCTTAGATGGGAGGCTGAACGTGATGACTGGCTACACAACAGAGA TGCAAAAAGTATCATCAAGAAAAAGAAACATTTTAAAAAGAAGAGGATTAAAACCACTCAAAAAACTAAA AAACAAAGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205283 representing NM_005760
Red=Cloning site Green=Tags(s) MAAVKEPLEFHAKRPWRPEEAVEDPDEEDEDNTSEAENGFSLEEVLRLGGTKQDYLMLATLDENEEVIDG GKKGAIDDLQQGELEAFIQNLNLAKYTKASLVEEDEPAEKENSSKKEVKIPKINNKNTAESQRTSVNKVK NKNRPEPHSDENGSTTPKVKKDKQNIFEFFERQTLLLRPGGKWYDLEYSNEYSLKPQPQDVVSKYKTLAQ KLYQHEINLFKSKTNSQKGASSTWMKAIVSSGTLGDRMAAMILLIQDDAVHTLQFVETLVNLVKKKGSKQ QCLMALDTFKELLITDLLPDNRKLRIFSQRPFDKLEQLSSGNKDSRDRRLILWYFEHQLKHLVAEFVQVL ETLSHDTLVTTKTRALTVAHELLCNKPEEEKALLVQVVNKLGDPQNRIATKASHLLETLLCKHPNMKGVV SGEVERLLFRSNISSKAQYYAICFLNQMALSHEESELANKLITVYFCFFRTCVKKKDVESKMLSALLTGV NRAYPYSQTGDDKVREQIDTLFKVLHIVNFNTSVQALMLLFQVMNSQQTISDRYYTALYRKMLDPGLMTC SKQAMFLNLVYKSLKADIVLRRVKAFVKRLLQVTCQQMPPFICGALYLVSEILKAKPGLRSQLDDHPESD DEENFIDANDDEDMEKFTDADKETEIVKKLETEETVPETDVETKKPEVASWVHFDNLKGGKQLNKYDPFS RNPLFCGAENTSLWELKKLSVHFHPSVALFAKTILQGNYIQYSGDPLQDFTLMRFLDRFVYRNPKPHKGK ENTDSVVMQPKRKHFIKDIRHLPVNSKEFLAKEESQIPVDEVFFHRYYKKVAVKEKQKRDADEESIEDVD DEEFEELIDTFEDDNCFSSGKDDMDFAGNVKKRTKGAKDNTLDEDSEGSDDELGNLDDDEVSLGSMDDEE FAEVDEDGGTFMDVLDDESESVPELEVHSKVSTKKSKRKGTDDFDFAGSFQGPRKKKRNLNDSSLFVSAE EFGHLLDENMGSKFDNIGMNAMANKDNASLKQLRWEAERDDWLHNRDAKSIIKKKKHFKKKRIKTTQKTK KQRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005760 |
| ORF Size | 3162 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005760.3 |
| RefSeq Size | 3341 bp |
| RefSeq ORF | 3165 bp |
| Locus ID | 10153 |
| UniProt ID | Q03701 |
| Domains | CBF |
| Protein Families | Stem cell - Pluripotency, Transcription Factors |
| MW | 121.4 kDa |
| Gene Summary | This gene belongs to the CBF/Mak21 family. The encoded protein plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner. The protein is also involved in cell growth and differentiation, particularly, hematopoietic differentiation. [provided by RefSeq, Nov 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205283L3 | Lenti-ORF clone of CEBPZ (Myc-DDK-tagged)-Human CCAAT/enhancer binding protein (C/EBP), zeta (CEBPZ) |
CNY 12,450.00 |
|
| RC205283L4 | Lenti-ORF clone of CEBPZ (mGFP-tagged)-Human CCAAT/enhancer binding protein (C/EBP), zeta (CEBPZ) |
CNY 12,450.00 |
|
| RG205283 | CEBPZ (tGFP-tagged) - Human CCAAT/enhancer binding protein (C/EBP), zeta (CEBPZ) |
CNY 8,936.00 |
|
| SC127963 | CEBPZ (untagged)-Human CCAAT/enhancer binding protein (C/EBP), zeta (CEBPZ) |
CNY 7,344.00 |
