Liprin alpha 1 (PPFIA1) (NM_003626) Human Tagged ORF Clone
CAT#: RC205488
PPFIA1 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (PPFIA1), transcript variant 2
ORF Plasmid: tGFP
"NM_003626" in other vectors (4)
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CNY 8,368.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | LIP.1; LIP1; LIPRIN |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205488 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGCGAGGTGATGCCGACCATCAGCGAAGCAGAAGGCCCCCCTGGAGGAGGTGGAGGCCATGGTT CCGGCTCCCCTTCACAGCCAGATGCAGATTCACATTTTGAACAGTTGATGGTCTCCATGCTAGAAGAAAG GGACCGCCTTCTTGATACACTGAGAGAGACTCAAGAAACGCTGGCCTTAACCCAGGGGAAGTTACACGAG GTTGGTCATGAAAGAGATTCCTTGCAGAGACAGCTCAACACAGCACTTCCACAGGAGTTCGCAGCACTTA CTAAAGAACTCAATGTATGCAGGGAACAGCTCCTTGAAAGGGAAGAAGAAATTGCTGAACTGAAAGCAGA AAGGAATAACACCAGGCTGCTGTTAGAGCATTTGGAATGCCTTGTCTCCAGGCATGAGCGGTCTCTTAGG ATGACCGTGGTGAAGAGACAAGCGCAGTCTCCAGCAGGCGTGTCCAGCGAAGTGGAAGTGCTGAAAGCAC TGAAGTCCTTATTTGAACACCACAAAGCTCTGGATGAAAAGGTGAGAGAGCGATTACGAGTAGCACTTGA AAGATGTAGTTTGTTAGAAGAGGAATTAGGTGCCACACACAAAGAGCTAATGATTCTTAAAGAACAGAAT AATCAGAAAAAAACTCTAACAGATGGAGTGCTGGACATAAACCATGAACAAGAAAATACACCAAGCACGA GTGGAAAGAGATCTTCTGATGGTTCTTTAAGCCACGAGGAAGACCTTGCTAAAGTAATTGAGCTCCAAGA AATCATAAGTAAGCAGTCAAGGGAACAGAGCCAAATGAAAGAACGCCTGGCTTCCCTTTCCAGTCATGTG ACAGAACTGGAAGAGGATCTGGACACGGCTAGAAAAGATCTCATCAAATCTGAAGAAATGAACACAAAAT TGCAACGAGATGTCCGTGAAGCCATGGCCCAAAAGGAAGATATGGAAGAGAGAATCACTACTCTTGAAAA ACGCTACCTCGCTGCACAGCGTGAAGCCACATCTGTGCATGACCTCAATGATAAACTTGAAAATGAAATT GCAAATAAAGATTCTATGCATCGACAGACTGAAGATAAAAACCGCCAGTTACAGGAGCGCTTGGAATTGG CAGAGCAAAAGCTGCAACAGACACTGAGGAAGGCAGAGACGCTCCCGGAGGTGGAGGCGGAGCTGGCCCA GAGGGTGGCAGCGCTTTCCAAGGCTGAAGAGAGACACGGCAACATTGAAGAAAGGTTACGACAGATGGAA GCACAGTTGGAGGAGAAGAATCAAGAACTGCAGCGGGCAAGGCAAAGAGAAAAAATGAACGAAGAACATA ATAAACGTTTATCAGACACTGTTGACAAGCTGCTTTCAGAATCTAATGAGAGGCTTCAACTTCATCTTAA AGAGAGAATGGCTGCTTTGGAAGATAAGAACTCTCTTTTAAGAGAAGTTGAAAGTGCAAAAAAGCAGTTA GAAGGAACACAACACGATAAGGATCAGCTTGTCCTAAACATTGAAGCACTGAGGGCTGAACTAGACCACA TGAGACTAAGAGGTGCTTCACTTCATCATGGCCGACCCCACTTGGGCAGTGTCCCAGATTTCAGGTTCCC CATGGCAGATGGCCACACAGACTCCTACAGCACCAGTGCAGTGCTGCGGCGCACACAGAAAGGCCGGCTG GCAGCCCTGCGAGATGAGCCTTCCAAGGTACAAACTCTTAATGAGCAGGATTGGGAACGTGCCCAGCAAG CTAGTGTCTTGGCAAATGTAGCACAAGCATTCGAGAGTGATGCTGACGTGTCTGATGGTGAAGATGACAG GGACACTCTCCTCAGCTCAGTTGACCTGCTATCGCCCAGCGGGCAGGCCGACGCGCACACACTAGCCATG ATGCTTCAGGAGCAGCTGGACGCCATCAACAAAGAGATCAGGTTGATTCAGGAAGAAAAAGAAAATACAG AGCAGCGGGCAGAGGAGATTGAAAGTCGAGTTGGCAGTGGAAGTCTAGACAATCTTGGTCGTTTTAGATC AATGAGCTCCATTCCCCCCTACCCTGCTTCCTCGCTTGCTAGCTCCTCCCCTCCGGGCAGTGGGCGCTCC ACCCCACGAAGGATCCCTCACAGCCCAGCTCGGGAAGTGGACAGACTGGGCGTCATGACCCTTTTGCCAC CTTCCAGAGAAGAGGTACGAGATGACAAGACAACCATAAAGTGTGAAACCTCCCCGCCTTCCTCCCCGAG AGCCCTTCGGTTAGACCGGCTGCACAAAGGGGCGCTGCACACCGTTAGCCACGAGGACATCAGGGACATA AGGAACTCCACAGGCTCCCAGGATGGTCCCGTGAGCAACCCCAGCAGTAGCAACAGTAGCCAGGACTCGC TCCACAAAGCCCCAAAGAAGAAAGGCATTAAGTCCTCCATTGGCCGCTTGTTTGGCAAGAAAGAAAAGGG CCGACCTGGACAAACTGGCAAAGAAGCATTAGGACAAGCTGGTGTTTCCGAGACGGATAACTCATCTCAG GATGCCTTGGGACTTAGCAAATTGGGGGGACAGGCTGAAAAAAATCGTAAACTTCAAAAAAAGCATGAAT TGCTGGGGGAAGCCCGGAGACAAGGTTTACCTTTTGCCCAATGGGACGGGCCAACGGTTGTGGTCTGGCT AGAGCTCTGGGTTGGGATGCCAGCCTGGTATGTGGCTGCCTGCCGAGCAAACGTGAAAAGCGGGGCCATC ATGTCGGCCCTGTCCGACACAGAGATCCAGCGTGAGATTGGCATCAGCAACCCCCTGCACAGGCTGAAGC TGAGGCTGGCCATCCAGGAGATCATGTCGCTGACCAGCCCGTCTGCCCCGCCCACATCTAGAACGACACT CGCCTATGGGGACATGAACCACGAGTGGATCGGCAACGAGTGGCTCCCCAGCCTGGGCCTCCCCCAGTAC CGCAGCTACTTCATGGAGTGCCTTGTAGACGCCAGGATGCTGGACCACTTGACCAAGAAAGACCTTCGAG GGCAGCTGAAAATGGTCGACAGTTTTCACAGAAACAGTTTCCAGTGTGGAATTATGTGCCTGAGAAGGTT AAATTATGACCGGAAAGAACTGGAAAGAAAAAGAGAAGAAAGTCAGAGTGAAATAAAAGACGTGCTTGTT TGGAGCAATGATCGAGTGATTCGCTGGATCCTGTCAATTGGCCTTAAAGAATATGCAAACAATCTTATAG AGAGTGGTGTTCACGGAGCACTTCTGGCCTTAGATGAAACCTTCGACTTCAGTGCACTGGCACTGCTGTT ACAGATCCCAACGCAGAACACACAGGCTCGTGCTGTCTTGGAAAGAGAATTTAACAACCTTTTGGTCATG GGGACTGATAGAAGGTTTGATGAAGATGATGATAAAAGCTTTAGGAGAGCACCTTCATGGAGAAAAAAGT TTAGACCAAAGGACATTCGTGGCTTAGCTGCTGGGTCAGCAGAGACTCTCCCTGCAAACTTCCGGGTGAC TTCTTCTATGTCTTCCCCCTCTATGCAGCCAAAGAAGATGCAGATGGACGGCAATGTATCAGGAACACAG AGGTTGGATTCTGCTACAGTCAGGACTTACTCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205488 protein sequence
Red=Cloning site Green=Tags(s) MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHE VGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLR MTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQN NQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHV TELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEI ANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQME AQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQL EGTQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRTQKGRL AALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAM MLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRS TPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDI RNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQ DALGLSKLGGQAEKNRKLQKKHELLGEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAI MSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQY RSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLV WSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVM GTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQ RLDSATVRTYSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003626 |
ORF Size | 3606 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003626.5 |
RefSeq Size | 5353 bp |
RefSeq ORF | 3609 bp |
Locus ID | 8500 |
UniProt ID | Q13136 |
Domains | SAM |
Protein Families | Druggable Genome, Phosphatase |
MW | 135.6 kDa |
Gene Summary | The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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PPFIA1 drives active a5ß1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis
,Mana, G;Clapero, F;Panieri, E;Panero, V;Böttcher, RT;Tseng, HY;Saltarin, F;Astanina, E;Wolanska, KI;Morgan, MR;Humphries, MJ;Santoro, MM;Serini, G;Valdembri, D;,
Nat Commun
,PubMed ID 27876801
[Liprin alpha 1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205488L3 | Lenti ORF clone of Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (PPFIA1), transcript variant 2, Myc-DDK-tagged |
CNY 12,160.00 |
|
RC205488L4 | Lenti ORF clone of Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (PPFIA1), transcript variant 2, mGFP tagged |
CNY 12,160.00 |
|
RG205488 | PPFIA1 (tGFP-tagged) - Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (PPFIA1), transcript variant 2 |
CNY 9,968.00 |
|
SC111709 | PPFIA1 (untagged)-Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (PPFIA1), transcript variant 2 |
CNY 8,376.00 |
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