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RNF133 (NM_139175) Human Tagged ORF Clone
CAT＃: RC205589
RNF133 (Myc-DDK-tagged)-Human ring finger protein 133 (RNF133)
ORF Plasmid: tGFP
"NM_139175" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205589 representing NM_139175
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCTACTCAAGGTTGGCACTTGGAGAAACAACACTGCCTCTTCCTGGCTTATGAAGTTCAGTGTTC TTTGGCTTGTTAGTCAGAACTGTTGCAGAGCAAGTGTTGTTTGGATGGCTTATATGAACATATCATTTCA TGTTGGGAATCATGTGTTGTCAGAGTTGGGAGAGACTGGAGTCTTTGGAAGAAGCTCCACTTTGAAGAGA GTGGCAGGAGTTATAGTGCCACCAGAGGGAAAAATCCAAAATGCATGTAATCCCAATACCATTTTCAGCC GATCAAAGTACTCAGAGACCTGGCTTGCACTTATTGAACGGGGAGGTTGTACCTTCACACAGAAAATTAA AGTGGCAACTGAGAAGGGAGCCAGTGGAGTGATCATCTATAACGTTCCAGGTACTGGCAACCAGGTGTTC CCCATGTTTCATCAGGCATTTGAAGATGTCGTTGTGGTTATGATTGGTAACTTAAAAGGCACGGAAATTT TCCATTTAATTAAGAAGGGAGTTCTCATTACAGCCGTGGTTGAGGTGGGGAGAAAGCACATCATCTGGAT GAATCACTATTTGGTCTCTTTTGTGATTGTCACAACTGCTACCTTAGCATATTTCATCTTTTATCACATT CATAGACTTTGTTTAGCAAGGATTCAGAACCGGAGATGGCAGCGATTAACAACAGATCTTCAGAACACAT TTGGACAACTCCAACTTCGAGTAGTAAAAGAGGGGGATGAAGAAATAAATCCAAATGGGGATAGCTGCGT AATTTGCTTTGAACGCTATAAGCCTAATGACATAGTTCGTATTCTGACTTGTAAACATTTTTTCCACAAG AATTGCATTGACCCCTGGATTTTACCCCATGGGACATGCCCCATTTGCAAATGTGATATTCTTAAAGTTT TGGGGATTCAAGTGGTTGTTGAAAATGGAACAGAACCTTTGCAAGTTCTAATGTCAAATGAACTGCCTGA AACCTTATCACCTAGTGAAGAGGAGACAAATAATGAAGTTTCTCCTGCAGGAACCTCAGATAAAGTAATC CATGTGGAGGAGAACCCTACTTCTCAGAATAATGACATCCAGCCTCATTCAGTAGTGGAAGATGTTCATC CTTCACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205589 representing NM_139175
Red=Cloning site Green=Tags(s) MHLLKVGTWRNNTASSWLMKFSVLWLVSQNCCRASVVWMAYMNISFHVGNHVLSELGETGVFGRSSTLKR VAGVIVPPEGKIQNACNPNTIFSRSKYSETWLALIERGGCTFTQKIKVATEKGASGVIIYNVPGTGNQVF PMFHQAFEDVVVVMIGNLKGTEIFHLIKKGVLITAVVEVGRKHIIWMNHYLVSFVIVTTATLAYFIFYHI HRLCLARIQNRRWQRLTTDLQNTFGQLQLRVVKEGDEEINPNGDSCVICFERYKPNDIVRILTCKHFFHK NCIDPWILPHGTCPICKCDILKVLGIQVVVENGTEPLQVLMSNELPETLSPSEEETNNEVSPAGTSDKVI HVEENPTSQNNDIQPHSVVEDVHPSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139175 |
| ORF Size | 1128 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139175.2 |
| RefSeq Size | 1446 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 168433 |
| UniProt ID | Q8WVZ7 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 42.1 kDa |
| Gene Summary | The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene has no intron. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205589L3 | Lenti ORF clone of Human ring finger protein 133 (RNF133), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205589L4 | Lenti ORF clone of Human ring finger protein 133 (RNF133), mGFP tagged |
CNY 5,890.00 |
|
| RG205589 | RNF133 (tGFP-tagged) - Human ring finger protein 133 (RNF133) |
CNY 5,256.00 |
|
| SC123164 | RNF133 (untagged)-Human ring finger protein 133 (RNF133) |
CNY 3,656.00 |
