BCL7C (NM_004765) Human Tagged ORF Clone

CAT#: RC205720

BCL7C (Myc-DDK-tagged)-Human B-cell CLL/lymphoma 7C (BCL7C)

ORF Plasmid: DDK tGFP



  "NM_004765" in other vectors (4)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal BCL7C Antibody
    • 100 ul

CNY 4,323.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205720 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCCGGACTGTACGGGCCGAGACCCGGAGCCGGGCCAAGGATGACATCAAGAAGGTGATGGCGA
CCATCGAGAAGGTCCGGAGATGGGAGAAGCGATGGGTGACTGTGGGCGACACTTCCCTTCGTATCTTCAA
GTGGGTGCCAGTGGTGGATCCCCAGGAGGAGGAGCGAAGGCGGGCAGGTGGCGGGGCAGAGAGATCCCGT
GGCCGGGAACGTCGGGGCAGGGGCGCCAGTCCCCGAGGGGGTGGCCCTCTCATCCTGCTGGATCTTAATG
ATGAGAACAGCAACCAGAGTTTCCATTCGGAAGGTTCCCTGCAAAAGGGCACAGAGCCCAGTCCTGGGGG
CACCCCCCAGCCCAGCCGCCCTGTGTCACCTGCCGGACCCCCAGAAGGGGTCCCTGAGGAGGCTCAGCCC
CCACGGCTGGGCCAAGAGAGAGATCCCGGGGGCATAACTGCTGGCAGCACCGACGAACCCCCAATGCTGA
CCAAGGAGGAGCCTGTTCCAGAACTGCTGGAAGCTGAGGCCCCCGAAGCTTACCCTGTCTTTGAGCCAGT
GCCACCTGTCCCTGAGGCAGCCCAGGGTGACACAGAGGACTCGGAGGGTGCCCCCCCACTCAAGCGCATC
TGCCCAAATGCCCCTGACCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205720 protein sequence
Red=Cloning site Green=Tags(s)

MAGRTVRAETRSRAKDDIKKVMATIEKVRRWEKRWVTVGDTSLRIFKWVPVVDPQEEERRRAGGGAERSR
GRERRGRGASPRGGGPLILLDLNDENSNQSFHSEGSLQKGTEPSPGGTPQPSRPVSPAGPPEGVPEEAQP
PRLGQERDPGGITAGSTDEPPMLTKEEPVPELLEAEAPEAYPVFEPVPPVPEAAQGDTEDSEGAPPLKRI
CPNAPDP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004765
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004765.4
RefSeq Size 1101 bp
RefSeq ORF 654 bp
Locus ID 9274
UniProt ID Q8WUZ0
Domains BCL_N
MW 23.5 kDa
Gene Summary This gene is identified by the similarity of its product to the N-terminal region of BCL7A protein. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. The function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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