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ABHD4 (NM_022060) Human Tagged ORF Clone
CAT＃: RC205745
ABHD4 (Myc-DDK-tagged)-Human abhydrolase domain containing 4 (ABHD4)
ORF Plasmid: tGFP
"NM_022060" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ABH4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205745 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGATGATCTGGAGCAGCAGTCTCAAGGCTGGCTGAGTAGCTGGCTGCCCACGTGGCGCCCCACTT CCATGTCTCAGCTGAAGAATGTGGAAGCCAGGATCCTCCAGTGTCTCCAGAATAAGTTCCTGGCCAGATA TGTATCCCTCCCAAACCAGAATAAGATCTGGACGGTGACTGTGAGCCCCGAGCAAAACGACCGCACCCCC TTGGTGATGGTGCATGGTTTTGGGGGCGGCGTGGGTCTCTGGATCCTCAACATGGACTCACTGAGTGCCC GCCGCACACTGCACACCTTCGATCTGCTTGGCTTCGGGCGAAGCTCAAGGCCAGCATTCCCAAGGGACCC GGAGGGGGCTGAGGATGAGTTTGTGACATCGATAGAGACATGGCGGGAGACCATGGGGATCCCCAGCATG ATCCTCCTGGGGCACAGTTTGGGAGGATTCCTGGCCACTTCTTACTCAATCAAGTACCCTGATAGAGTTA AACACCTCATCCTGGTGGACCCATGGGGCTTTCCCCTCCGACCAACTAACCCCAGTGAGATCCGTGCACC CCCAGCCTGGGTCAAAGCCGTGGCATCTGTCCTAGGACGTTCCAATCCATTGGCTGTTCTTCGAGTAGCT GGGCCCTGGGGGCCTGGTCTGGTGCAGCGATTCCGGCCGGACTTCAAACGCAAGTTTGCAGACTTCTTTG AAGATGATACCATATCAGAGTATATTTACCACTGCAACGCACAGAATCCCAGTGGTGAGACAGCATTCAA AGCCATGATGGAGTCCTTTGGCTGGGCCCGGCGCCCTATGCTGGAGCGAATTCACTTGATTCGAAAAGAT GTGCCTATCACTATGATCTACGGGTCCGACACCTGGATAGATACCAGTACGGGAAAAAAGGTGAAGATGC AGCGGCCGGATTCCTATGTCCGAGACATGGAGATTAAGGGTGCCTCCCACCATGTCTATGCTGACCAGCC ACACATCTTCAATGCTGTGGTGGAGGAGATCTGCGACTCAGTTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205745 protein sequence
Red=Cloning site Green=Tags(s) MADDLEQQSQGWLSSWLPTWRPTSMSQLKNVEARILQCLQNKFLARYVSLPNQNKIWTVTVSPEQNDRTP LVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSM ILLGHSLGGFLATSYSIKYPDRVKHLILVDPWGFPLRPTNPSEIRAPPAWVKAVASVLGRSNPLAVLRVA GPWGPGLVQRFRPDFKRKFADFFEDDTISEYIYHCNAQNPSGETAFKAMMESFGWARRPMLERIHLIRKD VPITMIYGSDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHHVYADQPHIFNAVVEEICDSVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022060 |
| ORF Size | 1026 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022060.2, NP_071343.2 |
| RefSeq Size | 2543 bp |
| RefSeq ORF | 1029 bp |
| Locus ID | 63874 |
| UniProt ID | Q8TB40 |
| Domains | abhydrolase |
| Protein Families | Protease |
| MW | 38.8 kDa |
| Gene Summary | Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205745L1 | Lenti ORF clone of Human abhydrolase domain containing 4 (ABHD4), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205745L2 | Lenti ORF clone of Human abhydrolase domain containing 4 (ABHD4), mGFP tagged |
CNY 5,890.00 |
|
| RC205745L3 | Lenti ORF clone of Human abhydrolase domain containing 4 (ABHD4), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205745L4 | Lenti ORF clone of Human abhydrolase domain containing 4 (ABHD4), mGFP tagged |
CNY 5,890.00 |
|
| RG205745 | ABHD4 (tGFP-tagged) - Human abhydrolase domain containing 4 (ABHD4) |
CNY 5,256.00 |
|
| SC112859 | ABHD4 (untagged)-Human abhydrolase domain containing 4 (ABHD4) |
CNY 3,656.00 |
