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Myosin Light Chain 2 (MYL2) (NM_000432) Human Tagged ORF Clone
CAT＃: RC205932
MYL2 (Myc-DDK-tagged)-Human myosin, light chain 2, regulatory, cardiac, slow (MYL2)
ORF Plasmid: tGFP
"NM_000432" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 6,193.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CMH10; MLC-2s/v; MLC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205932 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCTAAGAAAGCAAAGAAGAGAGCCGGGGGCGCCAACTCCAACGTGTTCTCCATGTTCGAACAGA CCCAAATCCAGGAATTTAAGGAGGCCTTCACTATCATGGACCAGAACAGGGATGGCTTCATTGACAAGAA CGATCTGAGAGACACCTTTGCTGCCCTTGGGCGAGTGAACGTGAAAAATGAAGAAATTGATGAAATGATC AAGGAGGCTCCGGGTCCAATTAACTTTACTGTGTTCCTCACAATGTTTGGGGAGAAACTTAAGGGAGCGG ACCCTGAGGAAACCATTCTCAACGCATTCAAAGTGTTTGACCCTGAAGGCAAAGGGGTGCTGAAGGCTGA TTACGTTCGGGAAATGCTGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGAGGTTGACCAGATGTTCGCC GCCTTCCCCCCTGACGTGACTGGCAACTTGGACTACAAGAACCTGGTGCACATCATCACCCACGGAGAAG AGAAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205932 protein sequence
Red=Cloning site Green=Tags(s) MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMI KEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFA AFPPDVTGNLDYKNLVHIITHGEEKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000432 |
| ORF Size | 498 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000432.4 |
| RefSeq Size | 855 bp |
| RefSeq ORF | 501 bp |
| Locus ID | 4633 |
| UniProt ID | P10916 |
| Domains | EFh |
| Protein Pathways | Cardiac muscle contraction, Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction |
| MW | 18.8 kDa |
| Gene Summary | Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of MYLK3 mutations in familial dilated cardiomyopathy
,Tobita, T;Nomura, S;Morita, H;Ko, T;Fujita, T;Toko, H;Uto, K;Hagiwara, N;Aburatani, H;Komuro, I;,
Sci Rep
,PubMed ID 29235529
[Myosin Light Chain 2]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205932L1 | Lenti ORF clone of Human myosin, light chain 2, regulatory, cardiac, slow (MYL2), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC205932L2 | Lenti ORF clone of Human myosin, light chain 2, regulatory, cardiac, slow (MYL2), mGFP tagged |
CNY 5,890.00 |
|
| RC205932L3 | Lenti ORF clone of Human myosin, light chain 2, regulatory, cardiac, slow (MYL2), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC205932L4 | Lenti ORF clone of Human myosin, light chain 2, regulatory, cardiac, slow (MYL2), mGFP tagged |
CNY 3,600.00 |
|
| RG205932 | MYL2 (tGFP-tagged) - Human myosin, light chain 2, regulatory, cardiac, slow (MYL2) |
CNY 2,800.00 |
|
| SC119884 | MYL2 (untagged)-Human myosin, light chain 2, regulatory, cardiac, slow (MYL2) |
CNY 2,400.00 |
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