NISCH (NM_007184) Human Tagged Lenti ORF Clone
CAT#: RC206026L3
- LentiORF®
Lenti ORF clone of Human nischarin (NISCH), Myc-DDK-tagged
"NM_007184" in other vectors (6)
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CNY 14,730.00
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CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | hIRAS; I-1; IR1; IRAS |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC206026).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_007184 |
ORF Size | 4512 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007184.2 |
RefSeq Size | 5252 bp |
RefSeq ORF | 4515 bp |
Locus ID | 11188 |
UniProt ID | Q9Y2I1 |
Domains | LRR, PX, LRR_SD22 |
Protein Families | Druggable Genome |
MW | 166.7 kDa |
Gene Summary | This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206026 | NISCH (Myc-DDK-tagged)-Human nischarin (NISCH) |
CNY 10,288.00 |
|
RC206026L1 | Lenti ORF clone of Human nischarin (NISCH), Myc-DDK-tagged |
CNY 12,688.00 |
|
RC206026L2 | Lenti ORF clone of Human nischarin (NISCH), mGFP tagged |
CNY 14,730.00 |
|
RC206026L4 | Lenti ORF clone of Human nischarin (NISCH), mGFP tagged |
CNY 14,730.00 |
|
RG206026 | NISCH (tGFP-tagged) - Human nischarin (NISCH) |
CNY 11,888.00 |
|
SC108496 | NISCH (untagged)-Human nischarin (NISCH) |
CNY 10,296.00 |