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NISCH (NM_007184) Human Tagged ORF Clone
CAT＃: RG206026
- TrueORF®
NISCH (tGFP-tagged) - Human nischarin (NISCH)
ORF Plasmid: DDK
"NM_007184" in other vectors (6)
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CNY 11,888.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | hIRAS; I-1; IR1; IRAS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206026 representing NM_007184
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGCGCGCACCTTCGGGCCCGAGCGGGAAGCCGAGCCGGCCAAGGAAGCGCGCGTCGTGGGCT CGGAGCTTGTGGACACTTATACGGTTTACATCATCCAGGTCACTGATGGCAGCCATGAGTGGACAGTAAA GCACCGCTACAGCGACTTCCATGACCTGCATGAAAAGCTCGTTGCAGAGAGAAAGATTGATAAAAATCTG CTTCCGCCCAAAAAGATAATTGGGAAAAACTCAAGAAGCTTGGTGGAGAAGAGGGAGAAGGATCTGGAGG TCTACCTCCAGAAGCTCCTGGCTGCCTTCCCTGGCGTGACCCCCAGAGTACTGGCCCACTTCTTGCATTT TCACTTCTATGAGATAAATGGCATCACCGCGGCACTGGCTGAAGAGCTCTTTGAGAAAGGAGAACAGCTC CTGGGGGCCGGCGAGGTCTTTGCCATTGGACCCCTGCAGCTGTATGCCGTCACGGAGCAGCTGCAGCAGG GAAAGCCCACGTGCGCCAGTGGGGATGCCAAGACCGACCTCGGGCACATCCTGGACTTCACCTGTCGCCT TAAGTACCTTAAGGTTTCTGGCACAGAAGGACCTTTTGGGACCAGCAACATTCAGGAGCAGCTCCTGCCG TTCGACCTATCAATATTCAAGTCCCTGCATCAGGTGGAGATAAGTCACTGTGATGCTAAGCACATCAGAG GGCTGGTCGCATCGAAGCCCACCTTAGCCACGCTGAGTGTCCGCTTCTCAGCAACCTCGATGAAGGAAGT CCTTGTTCCTGAAGCCTCAGAATTTGATGAGTGGGAGCCTGAAGGCACAACCCTAGAAGGCCCTGTGACT GCCGTCATCCCCACTTGGCAGGCATTGACCACGCTTGACCTGAGCCACAACAGCATCTCCGAGATCGACG AGTCTGTGAAACTGATCCCAAAGATTGAGTTCCTGGACCTGAGTCACAATGGATTGCTGGTTGTGGACAA TCTGCAGCACCTGTATAACCTTGTGCATCTGGACCTGTCCTACAACAAGCTCTCCTCCTTGGAAGGGCTT CACACCAAGCTGGGGAACATCAAGACCTTAAACCTGGCAGGCAACCTCCTAGAGAGTCTGAGTGGCCTGC ACAAGCTCTACTCACTGGTCAACCTGGATCTCCGGGACAACAGGATCGAACAGATGGAGGAGGTCCGGAG CATAGGCAGCCTCCCGTGTCTGGAGCACGTGTCTCTGCTGAACAACCCTCTGAGCATCATCCCCGACTAC CGGACCAAGGTGCTGGCTCAGTTCGGAGAGAGGGCCTCAGAGGTCTGTCTGGATGACACAGTGACCACAG AGAAGGAGCTGGACACTGTGGAAGTGCTGAAAGCAATTCAGAAAGCCAAGGAGGTCAAGTCCAAACTGAG CAACCCAGAGAAGAAGGGTGGTGAAGACTCCCGGCTCTCAGCTGCCCCCTGCATCAGACCCAGCAGCTCC CCTCCCACTGTGGCTCCCGCATCTGCCTCCCTGCCCCAGCCCATCCTCTCTAACCAAGGAATCATGTTCG TTCAGGAGGAGGCCCTGGCCAGCAGCCTCTCGTCCACTGACAGTCTGACTCCCGAGCACCAGCCCATTGC CCAGGGATGTTCTGATTCCTTGGAGTCCATCCCTGCGGGACAGGCAGCTTCCGATGATTTAAGGGACGTG CCAGGAGCTGTTGGTGGTGCAAGCCCAGAACATGCCGAGCCGGAGGTCCAGGTGGTGCCGGGGTCTGGCC AGATCATCTTCCTGCCCTTCACCTGCATTGGCTACACGGCCACCAATCAGGACTTCATCCAGCGCCTGAG CACACTGATCCGGCAGGCCATCGAGCGGCAGCTGCCTGCCTGGATCGAGGCTGCCAACCAGCGGGAGGAG GGCCAGGGTGAACAGGGCGAGGAGGAGGATGAGGAGGAGGAAGAAGAGGAGGACGTGGCTGAGAACCGCT ACTTTGAAATGGGGCCCCCAGACGTGGAGGAGGAGGAGGGAGGAGGCCAGGGGGAGGAAGAGGAGGAGGA AGAGGAGGATGAAGAGGCCGAGGAGGAGCGCCTGGCTCTGGAATGGGCCCTGGGCGCGGACGAGGACTTC CTGCTGGAGCACATCCGCATCCTCAAGGTGCTGTGGTGCTTCCTGATCCATGTGCAGGGCAGTATCCGCC AGTTCGCCGCCTGCCTTGTGCTCACCGACTTCGGCATCGCAGTCTTCGAGATCCCGCACCAGGAGTCTCG GGGCAGCAGCCAGCACATCCTCTCCTCCCTGCGCTTTGTCTTTTGCTTCCCGCATGGCGACCTCACCGAG TTTGGCTTCCTCATGCCGGAGCTGTGTCTGGTGCTCAAGGTACGGCACAGTGAGAACACGCTCTTCATTA TCTCGGACGCCGCCAACCTGCACGAGTTCCACGCGGACCTGCGCTCATGCTTTGCACCCCAGCACATGGC CATGCTGTGTAGCCCCATCCTCTACGGCAGCCACACCAGCCTGCAGGAGTTCCTGCGCCAGCTGCTCACC TTCTACAAGGTGGCTGGCGGCTGCCAGGAGCGCAGCCAGGGCTGCTTCCCCGTCTACCTGGTCTACAGTG ACAAGCGCATGGTGCAGACGGCCGCCGGGGACTACTCAGGCAACATCGAGTGGGCCAGCTGCACACTCTG TTCAGCCGTGCGGCGCTCCTGCTGCGCGCCCTCTGAGGCCGTCAAGTCCGCCGCCATCCCCTACTGGCTG TTGCTCACGCCCCAGCACCTCAACGTCATCAAGGCCGACTTCAACCCCATGCCCAACCGTGGCACCCACA ACTGTCGCAACCGCAACAGCTTCAAGCTCAGCCGTGTGCCGCTCTCCACCGTGCTGCTGGACCCCACACG CAGCTGTACCCAGCCTCGGGGCGCCTTTGCTGATGGCCACGTGCTAGAGCTGCTCGTGGGGTACCGCTTT GTCACTGCCATCTTCGTGCTGCCCCACGAGAAGTTCCACTTCCTGCGCGTCTACAACCAGCTGCGGGCCT CGCTGCAGGACCTGAAGACTGTGGTCATCGCCAAGACCCCCGGGACGGGAGGCAGCCCCCAGGGCTCCTT TGCGGATGGCCAGCCTGCCGAGCGCAGGGCCAGCAATGACCAGCGTCCCCAGGAGGTCCCAGCAGAGGCT CTGGCCCCGGCCCCAGCGGAAGTCCCAGCTCCAGCCCCTGCAGCAGCCTCAGCCTCAGGCCCAGCGAAGA CTCCGGCCCCAGCAGAGGCCTCAACTTCAGCTTTGGTCCCAGAGGAGACGCCAGTGGAAGCTCCAGCCCC ACCCCCAGCCGAGGCCCCTGCCCAGTACCCGAGTGAGCACCTCATCCAGGCCACCTCGGAGGAGAATCAG ATCCCCTCGCACTTGCCTGCCTGCCCGTCGCTCCGGCACGTCGCCAGCCTGCGGGGCAGCGCCATCATCG AGCTCTTCCACAGCAGCATTGCTGAGGTTGAAAACGAGGAGCTGAGGCACCTCATGTGGTCCTCGGTGGT GTTCTACCAGACCCCAGGGCTGGAGGTGACTGCCTGCGTGCTGCTCTCCACCAAGGCTGTGTACTTTGTG CTCCACGACGGCCTCCGCCGCTACTTCTCAGAGCCACTGCAGGATTTCTGGCATCAGAAAAACACCGACT ACAACAACAGCCCTTTCCACATCTCCCAGTGCTTCGTGCTAAAGCTTAGTGACCTGCAGTCAGTCAATGT GGGGCTTTTCGACCAGCATTTCCGGCTGACGGGTTCCACCCCGATGCAGGTGGTCACGTGCTTGACGCGG GACAGCTACCTGACGCACTGCTTCCTCCAGCACCTCATGGTCGTGCTGTCCTCTCTGGAACGCACGCCCT CGCCGGAGCCTGTTGACAAGGACTTCTACTCCGAGTTTGGGAACAAGACCACAGGGAAGATGGAGAACTA CGAGCTGATCCACTCTAGTCGCGTCAAGTTTACCTACCCCAGTGAGGAGGAGATTGGGGACCTGACGTTC ACTGTGGCCCAAAAGATGGCTGAGCCAGAGAAGGCCCCAGCCCTCAGCATCCTGCTGTACGTGCAGGCCT TCCAGGTGGGCATGCCACCCCCTGGGTGCTGCAGGGGCCCCCTGCGCCCCAAGACACTCCTGCTCACCAG CTCCGAGATCTTCCTCCTGGATGAGGACTGTGTCCACTACCCACTGCCCGAGTTTGCCAAAGAGCCGCCG CAGAGAGACAGGTACCGGCTGGACGATGGCCGCCGCGTCCGGGACCTGGACCGAGTGCTCATGGGCTACC AGACCTACCCGCAGGCCCTCACCCTCGTCTTCGATGACGTGCAAGGTCATGACCTCATGGGCAGTGTCAC CCTGGACCACTTTGGGGAGGTGCCAGGTGGCCCGGCTAGAGCCAGCCAGGGCCGTGAAGTCCAGTGGCAG GTGTTTGTCCCCAGTGCTGAGAGCAGAGAGAAGCTCATCTCGCTGTTGGCTCGCCAGTGGGAGGCCCTGT GTGGCCGTGAGCTGCCTGTCGAGCTCACCGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206026 representing NM_007184
Red=Cloning site Green=Tags(s) MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKLVAERKIDKNL LPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQL LGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLP FDLSIFKSLHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVT AVIPTWQALTTLDLSHNSISEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGL HTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDY RTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSS PPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDV PGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREE GQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDF LLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTE FGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLT FYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWL LLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRF VTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEA LAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQ IPSHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFV LHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTR DSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTF TVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPP QRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQ VFVPSAESREKLISLLARQWEALCGRELPVELTG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007184 |
| ORF Size | 4512 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007184.3 |
| RefSeq Size | 5139 bp |
| RefSeq ORF | 4515 bp |
| Locus ID | 11188 |
| UniProt ID | Q9Y2I1 |
| Domains | LRR, PX, LRR_SD22 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206026 | NISCH (Myc-DDK-tagged)-Human nischarin (NISCH) |
CNY 10,288.00 |
|
| RC206026L1 | Lenti ORF clone of Human nischarin (NISCH), Myc-DDK-tagged |
CNY 12,688.00 |
|
| RC206026L2 | Lenti ORF clone of Human nischarin (NISCH), mGFP tagged |
CNY 14,730.00 |
|
| RC206026L3 | Lenti ORF clone of Human nischarin (NISCH), Myc-DDK-tagged |
CNY 14,730.00 |
|
| RC206026L4 | Lenti ORF clone of Human nischarin (NISCH), mGFP tagged |
CNY 14,730.00 |
|
| SC108496 | NISCH (untagged)-Human nischarin (NISCH) |
CNY 10,296.00 |
