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ZMPSTE24 (NM_005857) Human Tagged ORF Clone
CAT＃: RC206035
- TrueORF®
ZMPSTE24 (Myc-DDK-tagged)-Human zinc metallopeptidase (STE24 homolog, S. cerevisiae) (ZMPSTE24)
ORF Plasmid: tGFP
"NM_005857" in other vectors (6)
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CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FACE-1; FACE1; HGPS; PRO1; STE24; Ste24p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206035 representing NM_005857
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGATGTGGGCATCGCTGGACGCTTTGTGGGAGATGCCGGCCGAGAAGCGTATCTTCGGGGCCGTGC TGCTCTTTTCCTGGACAGTGTATCTTTGGGAGACCTTCCTAGCACAGCGGCAGAGAAGGATATATAAAAC AACAACTCATGTACCACCGGAGTTAGGACAGATCATGGATTCTGAAACATTTGAGAAATCTCGACTCTAT CAACTGGATAAAAGCACTTTCAGCTTCTGGTCAGGACTCTATTCAGAGACTGAAGGCACTCTTATTCTTC TCTTTGGAGGAATACCTTATCTCTGGAGACTTTCTGGACGGTTCTGTGGTTATGCTGGCTTTGGACCAGA ATATGAGATCACTCAGTCCCTGGTGTTTCTGCTGTTGGCTACACTTTTCAGTGCATTGGCTGGTTTGCCA TGGAGTCTTTATAATACTTTTGTGATAGAAGAAAAACATGGCTTCAATCAACAGACTTTGGGGTTCTTCA TGAAAGATGCAATCAAGAAATTTGTTGTGACTCAGTGCATTTTGTTGCCTGTGTCTTCACTTCTACTTTA CATTATTAAAATTGGGGGTGACTATTTTTTTATTTATGCCTGGCTGTTCACATTAGTTGTGTCTCTGGTT CTTGTCACAATCTATGCTGATTATATTGCCCCTTTATTTGACAAATTCACACCTCTGCCTGAGGGAAAGC TTAAAGAAGAAATTGAAGTAATGGCAAAGAGTATTGACTTTCCTTTGACGAAGGTGTATGTTGTGGAAGG ATCTAAACGCTCTTCCCACAGCAATGCTTATTTTTATGGCTTCTTCAAGAACAAGCGAATAGTTTTGTTT GACACTCTACTAGAAGAGTACTCTGTACTAAACAAAGACATCCAGGAGGATTCTGGCATGGAACCCCGCA ATGAGGAAGAAGGGAACAGTGAAGAAATAAAAGCTAAAGTTAAAAATAAGAAACAAGGATGTAAAAATGA GGAGGTACTCGCTGTACTAGGCCATGAACTGGGGCACTGGAAGTTGGGACATACAGTCAAAAATATCATT ATTAGCCAGATGAATTCTTTCCTGTGTTTTTTTTTATTTGCTGTATTAATTGGTCGAAAGGAGCTTTTTG CTGCATTTGGTTTTTATGATAGCCAACCCACTCTTATTGGACTATTGATCATCTTCCAGTTTATTTTTTC ACCTTACAATGAGGTTCTTTCTTTTTGCCTAACAGTCCTAAGCCGCAGATTTGAGTTTCAAGCTGATGCA TTTGCCAAGAAACTTGGGAAGGCTAAAGACTTATATTCTGCTTTAATCAAACTTAACAAAGATAACTTGG GATTCCCTGTTTCTGACTGGTTGTTCTCAATGTGGCATTATTCTCATCCTCCACTGCTAGAGAGACTTCA AGCTTTGAAAACTATGAAGCAACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206035 representing NM_005857
Red=Cloning site Green=Tags(s) MGMWASLDALWEMPAEKRIFGAVLLFSWTVYLWETFLAQRQRRIYKTTTHVPPELGQIMDSETFEKSRLY QLDKSTFSFWSGLYSETEGTLILLFGGIPYLWRLSGRFCGYAGFGPEYEITQSLVFLLLATLFSALAGLP WSLYNTFVIEEKHGFNQQTLGFFMKDAIKKFVVTQCILLPVSSLLLYIIKIGGDYFFIYAWLFTLVVSLV LVTIYADYIAPLFDKFTPLPEGKLKEEIEVMAKSIDFPLTKVYVVEGSKRSSHSNAYFYGFFKNKRIVLF DTLLEEYSVLNKDIQEDSGMEPRNEEEGNSEEIKAKVKNKKQGCKNEEVLAVLGHELGHWKLGHTVKNII ISQMNSFLCFFLFAVLIGRKELFAAFGFYDSQPTLIGLLIIFQFIFSPYNEVLSFCLTVLSRRFEFQADA FAKKLGKAKDLYSALIKLNKDNLGFPVSDWLFSMWHYSHPPLLERLQALKTMKQH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005857 |
| ORF Size | 1425 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005857.5 |
| RefSeq Size | 3154 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 10269 |
| UniProt ID | O75844 |
| Domains | Peptidase_M48 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 54.6 kDa |
| Gene Summary | This gene encodes a member of the peptidase M48A family. The encoded protein is a zinc metalloproteinase involved in the two step post-translational proteolytic cleavage of carboxy terminal residues of farnesylated prelamin A to form mature lamin A. Mutations in this gene have been associated with mandibuloacral dysplasia and restrictive dermopathy. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pregnancy-associated plasma protein A regulates mitosis and is epigenetically silenced in breast cancer
,Loddo, M;Andryszkiewicz, J;Rodriguez-Acebes, S;Stoeber, K;Jones, A;Dafou, D;Apostolidou, S;Wollenschlaeger, A;Widschwendter, M;Sainsbury, R;Tudzarova, S;Williams, GH;,
J. Pathol.
,PubMed ID 24931331
[ZMPSTE24]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206035L1 | Lenti ORF clone of Human zinc metallopeptidase (STE24 homolog, S. cerevisiae) (ZMPSTE24), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206035L2 | Lenti ORF clone of Human zinc metallopeptidase (STE24 homolog, S. cerevisiae) (ZMPSTE24), mGFP tagged |
CNY 5,890.00 |
|
| RC206035L3 | Lenti ORF clone of Human zinc metallopeptidase (STE24 homolog, S. cerevisiae) (ZMPSTE24), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206035L4 | Lenti ORF clone of Human zinc metallopeptidase (STE24 homolog, S. cerevisiae) (ZMPSTE24), mGFP tagged |
CNY 5,890.00 |
|
| RG206035 | ZMPSTE24 (tGFP-tagged) - Human zinc metallopeptidase (STE24 homolog, S. cerevisiae) (ZMPSTE24) |
CNY 7,088.00 |
|
| SC127925 | ZMPSTE24 (untagged)-Human zinc metallopeptidase (STE24 homolog, S. cerevisiae) (ZMPSTE24) |
CNY 11,016.00 |
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