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EDG2 (LPAR1) (NM_001401) Human Tagged ORF Clone
CAT＃: RC206065
LPAR1 (Myc-DDK-tagged)-Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1
ORF Plasmid: tGFP
"NM_001401" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | edg-2; EDG2; Gpcr26; LPA1; Mrec1.3; rec.1.3; vzg-1; VZG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206065 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCATCTCTACTTCCATCCCTGTAATTTCACAGCCCCAGTTCACAGCCATGAATGAACCACAGT GCTTCTACAACGAGTCCATTGCCTTCTTTTATAACCGAAGTGGAAAGCATCTTGCCACAGAATGGAACAC AGTCAGCAAGCTGGTGATGGGACTTGGAATCACTGTTTGTATCTTCATCATGTTGGCCAACCTGTTGGTC ATGGTGGCAATCTATGTCAACCGCCGCTTCCATTTTCCTATTTATTACCTAATGGCTAATCTGGCTGCTG CAGACTTCTTTGCTGGGTTGGCCTACTTCTATCTCATGTTCAACACAGGACCCAATACTCGGAGACTGAC TGTTAGCACATGGCTCCTTCGTCAGGGCCTCATTGACACCAGCCTGACGGCATCTGTGGCCAACTTACTG GCTATTGCAATCGAGAGGCACATTACGGTTTTCCGCATGCAGCTCCACACACGGATGAGCAACCGGCGGG TAGTGGTGGTCATTGTGGTCATCTGGACTATGGCCATCGTTATGGGTGCTATACCCAGTGTGGGCTGGAA CTGTATCTGTGATATTGAAAATTGTTCCAACATGGCACCCCTCTACAGTGACTCTTACTTAGTCTTCTGG GCCATTTTCAACTTGGTGACCTTTGTGGTAATGGTGGTTCTCTATGCTCACATCTTTGGCTATGTTCGCC AGAGGACTATGAGAATGTCTCGGCATAGTTCTGGACCCCGGCGGAATCGGGATACCATGATGAGTCTTCT GAAGACTGTGGTCATTGTGCTTGGGGCCTTTATCATCTGCTGGACTCCTGGATTGGTTTTGTTACTTCTA GACGTGTGCTGTCCACAGTGCGACGTGCTGGCCTATGAGAAATTCTTCCTTCTCCTTGCTGAATTCAACT CTGCCATGAACCCCATCATTTACTCCTACCGCGACAAAGAAATGAGCGCCACCTTTAGGCAGATCCTCTG CTGCCAGCGCAGTGAGAACCCCACCGGCCCCACAGAAGGCTCAGACCGCTCGGCTTCCTCCCTCAACCAC ACCATCTTGGCTGGAGTTCACAGCAATGACCACTCTGTGGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206065 protein sequence
Red=Cloning site Green=Tags(s) MAAISTSIPVISQPQFTAMNEPQCFYNESIAFFYNRSGKHLATEWNTVSKLVMGLGITVCIFIMLANLLV MVAIYVNRRFHFPIYYLMANLAAADFFAGLAYFYLMFNTGPNTRRLTVSTWLLRQGLIDTSLTASVANLL AIAIERHITVFRMQLHTRMSNRRVVVVIVVIWTMAIVMGAIPSVGWNCICDIENCSNMAPLYSDSYLVFW AIFNLVTFVVMVVLYAHIFGYVRQRTMRMSRHSSGPRRNRDTMMSLLKTVVIVLGAFIICWTPGLVLLLL DVCCPQCDVLAYEKFFLLLAEFNSAMNPIIYSYRDKEMSATFRQILCCQRSENPTGPTEGSDRSASSLNH TILAGVHSNDHSVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001401 |
| ORF Size | 1092 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001401.5 |
| RefSeq Size | 3104 bp |
| RefSeq ORF | 1095 bp |
| Locus ID | 1902 |
| UniProt ID | Q92633 |
| Domains | 7tm_1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Gap junction, Neuroactive ligand-receptor interaction |
| MW | 41.1 kDa |
| Gene Summary | The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Many transcript variants encoding a few different isoforms have been identified for this gene. [provided by RefSeq, Oct 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206065L1 | Lenti ORF clone of Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC206065L2 | Lenti ORF clone of Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206065L3 | Lenti ORF clone of Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206065L4 | Lenti ORF clone of Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG206065 | LPAR1 (tGFP-tagged) - Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1 |
CNY 7,088.00 |
|
| SC119263 | LPAR1 (untagged)-Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1 |
CNY 5,488.00 |
