DBF4 (NM_006716) Human Tagged ORF Clone
CAT#: RC206120
DBF4 (Myc-DDK-tagged)-Human DBF4 homolog (S. cerevisiae) (DBF4)
ORF Plasmid: tGFP
"NM_006716" in other vectors (6)
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CNY 4,936.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ASK; CHIF; DBF4A; ZDBF1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206120 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCCGGAGCCATGAGGATCCACAGTAAAGGACATTTCCAGGGTGGAATCCAAGTCAAAAATGAAA AAAACAGACCATCTCTGAAATCTCTGAAAACTGATAACAGGCCAGAAAAATCCAAATGTAAGCCACTTTG GGGAAAAGTATTTTACCTTGACTTACCTTCTGTCACCATATCTGAAAAACTTCAAAAGGACATTAAGGAT CTGGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATATCAGTTATCTTATTTCAAATAAGAAGGAAGCTA AATTTGCACAAACCTTGGGTCGAATTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAACCAC TTCACCTCATCCCAGCCATGATGGAAGTTCATTTAAGTCACCAGACACAGTGTGTTTAAGCAGAGGAAAA TTATTAGTTGAAAAAGCTATCAAGGACCATGATTTTATTCCTTCAAATAGTATATTATCAAATGCCTTGT CATGGGGAGTAAAAATTCTTCATATTGATGACATTAGATACTACATTGAACAAAAGAAAAAAGAGTTGTA TTTACTCAAGAAATCAAGTACTTCAGTAAGAGATGGGGGCAAAAGAGTTGGTAGTGGTGCACAAAAAACA AGAACAGGAAGACTCAAAAAGCCTTTTGTAAAGGTGGAAGATATGAGCCAACTTTATAGGCCATTTTATC TTCAGCTGACCAATATGCCTTTTATAAATTATTCTATTCAGAAGCCCTGCAGTCCATTTGATGTAGACAA GCCATCTAGTATGCAAAAGCAAACTCAGGTTAAACTAAGAATCCAAACAGATGGCGATAAGTATGGTGGA ACCTCAATTCAACTCCAGTTGAAAGAGAAGAAGAAAAAAGGATATTGTGAATGTTGCTTGCAGAAATATG AAGATCTAGAAACTCACCTTCTAAGTGAGCAACACAGAAACTTTGCACAGAGTAACCAGTATCAAGTTGT TGATGATATTGTATCTAAGTTAGTTTTTGACTTTGTGGAATATGAAAAGGACACACCTAAAAAGAAAAGA ATAAAATACAGTGTTGGATCCCTTTCTCCTGTTTCTGCAAGTGTCCTGAAAAAGACTGAACAAAAGGAAA AAGTGGAATTGCAACATATTTCTCAGAAAGATTGCCAGGAAGATGATACAACAGTGAAGGAGCAGAATTT CCTGTATAAAGAGACCCAGGAAACTGAAAAAAAGCTCCTGTTTATTTCAGAGCCCATCCCCCACCCTTCA AATGAATTGAGAGGGCTTAATGAGAAAATGAGTAATAAATGTTCCATGTTAAGTACAGCTGAAGATGACA TAAGACAGAATTTTACACAGCTACCTCTACATAAAAACAAACAGGAATGCATTCTTGACATTTCCGAACA CACATTAAGTGAAAATGACTTAGAAGAACTAAGGGTAGATCACTATAAATGTAACATACAGGCATCTGTA CATGTTTCTGATTTCAGTACAGATAATAGTGGATCTCAACCAAAACAGAAGTCAGATACTGTGCTTTTTC CAGCAAAGGATCTCAAGGAAAAGGACCTTCATTCAATATTTACTCATGATTCTGGTCTGATAACAATAAA CAGTTCACAAGAGCACCTAACTGTTCAGGCAAAGGCTCCATTCCATACTCCTCCTGAGGAACCCAATGAA TGTGACTTCAAGAATATGGATAGTTTACCTTCTGGTAAAATACATCGAAAAGTGAAAATAATATTAGGAC GAAATAGAAAAGAAAATCTGGAACCAAATGCTGAATTTGATAAAAGAACTGAATTTATTACACAAGAAGA AAACAGAATTTGTAGTTCACCGGTACAGTCTTTACTAGACTTGTTTCAGACTAGTGAAGAGAAATCAGAA TTTTTGGGTTTCACAAGCTACACAGAAAAGAGTGGTATATGCAATGTTTTAGATATTTGGGAAGAGGAAA ATTCAGATAATCTGTTAACAGCGTTTTTCTCGTCCCCTTCAACTTCTACATTTACTGGCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206120 protein sequence
Red=Cloning site Green=Tags(s) MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKD LGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGK LLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKT RTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGG TSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKR IKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPS NELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASV HVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNE CDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSE FLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006716 |
ORF Size | 2022 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006716.4 |
RefSeq Size | 3923 bp |
RefSeq ORF | 2025 bp |
Locus ID | 10926 |
UniProt ID | Q9UBU7 |
Domains | ZnF_DBF |
Protein Families | Druggable Genome |
Protein Pathways | Cell cycle |
MW | 76.9 kDa |
Gene Summary | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206120L1 | Lenti ORF clone of Human DBF4 homolog (S. cerevisiae) (DBF4), Myc-DDK-tagged |
CNY 7,336.00 |
|
RC206120L2 | Lenti ORF clone of Human DBF4 homolog (S. cerevisiae) (DBF4), mGFP tagged |
CNY 5,890.00 |
|
RC206120L3 | Lenti ORF clone of Human DBF4 homolog (S. cerevisiae) (DBF4), Myc-DDK-tagged |
CNY 7,336.00 |
|
RC206120L4 | Lenti ORF clone of Human DBF4 homolog (S. cerevisiae) (DBF4), mGFP tagged |
CNY 7,336.00 |
|
RG206120 | DBF4 (tGFP-tagged) - Human DBF4 homolog (S. cerevisiae) (DBF4) |
CNY 6,536.00 |
|
SC108298 | DBF4 (untagged)-Human DBF4 homolog (S. cerevisiae) (DBF4) |
CNY 4,944.00 |